FastQCFastQC Report
Wed 25 May 2016
SRR2088586_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088586_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145559
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT108570.9477469078414992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT102690.8964182551924431No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT75760.6613365178048446No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45370.3960511854911008No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG21820.19047469401401412No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA21120.18436414012722174No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG20380.17790441173261262No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA19980.17441266665444558No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA19760.17249220686145367No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG16800.1466532932830173No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13280.11592593659514701No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12490.10902974006576703No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA11500.10038767099730349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTTA502.7207716E-729.59999826
GTCGATT451.3232081E-424.66666818
CGAGTAT400.001931182823.12500226
GGTATCA36450.021.519891
CTATCGG656.902103E-519.92307712
CGTTTAT851.2453002E-619.58823627
ATCGGTC701.2191924E-418.514
TAAATCG500.0070348718.4999985
ACGAGTC500.0070348718.49999826
TAATACT2550.017.4117644
GTCTAGG752.0672582E-417.2666662
GCTCTAT752.0672582E-417.2666661
ATGCTAG1801.0913936E-1116.44444515
TAAGCCG700.002592485915.85714336
AGTACCG957.059645E-515.5789485
AATACTG2750.015.4727285
ACCGTTA1201.9353884E-615.4166668
CTAATAC2450.015.102043
ACTGTAC1353.9717997E-715.0740753
CTAGGCT1508.099596E-814.84
Produced by FastQC (version 0.11.5)