##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088586_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1145559
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.741046947385513	31.0	31.0	34.0	30.0	34.0
2	31.92790070175347	31.0	31.0	34.0	30.0	34.0
3	32.01336465428668	33.0	31.0	34.0	30.0	34.0
4	35.694094324255666	37.0	35.0	37.0	35.0	37.0
5	35.59464069506677	37.0	35.0	37.0	33.0	37.0
6	35.61873111729732	37.0	35.0	37.0	33.0	37.0
7	35.48973994355594	37.0	35.0	37.0	33.0	37.0
8	35.512578575175965	37.0	35.0	37.0	33.0	37.0
9	37.12845082619053	39.0	37.0	39.0	33.0	39.0
10	36.99853521293971	39.0	37.0	39.0	33.0	39.0
11	37.04552362645661	39.0	37.0	39.0	33.0	39.0
12	36.95166289994666	39.0	37.0	39.0	33.0	39.0
13	36.981418678566534	39.0	37.0	39.0	33.0	39.0
14	38.044935267410935	40.0	37.0	41.0	33.0	41.0
15	38.010602683929854	40.0	37.0	41.0	33.0	41.0
16	37.91550675259851	40.0	37.0	41.0	33.0	41.0
17	37.935806012610435	40.0	37.0	41.0	33.0	41.0
18	37.90538505655318	40.0	37.0	41.0	33.0	41.0
19	37.92974085140966	40.0	37.0	41.0	32.0	41.0
20	37.81739046177456	40.0	37.0	41.0	32.0	41.0
21	37.71992276259887	40.0	37.0	41.0	32.0	41.0
22	37.56489801049095	39.0	36.0	41.0	32.0	41.0
23	37.427322381474895	39.0	36.0	41.0	32.0	41.0
24	37.28878477668981	39.0	36.0	41.0	31.0	41.0
25	37.10327621711322	39.0	36.0	41.0	31.0	41.0
26	37.25832977611803	39.0	36.0	41.0	31.0	41.0
27	37.18465482790498	39.0	36.0	41.0	31.0	41.0
28	37.05886820320909	39.0	36.0	41.0	31.0	41.0
29	36.93358264393191	39.0	36.0	41.0	30.0	41.0
30	36.82029297487078	39.0	35.0	41.0	30.0	41.0
31	36.64240165718221	39.0	35.0	40.0	30.0	41.0
32	36.52418164407071	39.0	35.0	40.0	30.0	41.0
33	36.32660735937652	39.0	35.0	40.0	30.0	41.0
34	36.18431089101478	38.0	35.0	40.0	30.0	41.0
35	36.01720906561775	38.0	35.0	40.0	29.0	41.0
36	35.870025027082846	38.0	35.0	40.0	28.0	41.0
37	35.71996728234862	38.0	35.0	40.0	27.0	41.0
38	35.633239318097104	38.0	35.0	40.0	27.0	41.0
39	35.50914357095532	38.0	35.0	40.0	26.0	41.0
40	35.3376962688085	38.0	34.0	40.0	25.0	41.0
41	35.21958013511308	38.0	34.0	40.0	24.0	41.0
42	35.0184713314635	38.0	34.0	40.0	24.0	41.0
43	34.66811224912903	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	2.0
15	7.0
16	14.0
17	30.0
18	62.0
19	153.0
20	358.0
21	712.0
22	1360.0
23	2442.0
24	3797.0
25	5991.0
26	8762.0
27	12471.0
28	17189.0
29	22623.0
30	28644.0
31	35735.0
32	43834.0
33	54893.0
34	68144.0
35	84876.0
36	111040.0
37	157732.0
38	218867.0
39	265819.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.67345374616235	20.976571263461768	13.65612770708449	23.69384728329139
2	18.861184801481198	22.583559642061214	35.43073730816134	23.124518248296248
3	19.86811678839763	24.423098242866583	31.82228065075653	23.886504317979256
4	14.135718893570736	16.194626378911956	35.930056854339234	33.73959787317807
5	13.374780347411178	37.80337808877587	34.85311537860555	13.968726185207398
6	32.92113282685571	36.61653393670689	14.08124767035133	16.381085566086075
7	26.620191539676263	32.26145488796299	21.91916784731297	19.19918572504777
8	26.36939694943691	34.934822213434664	19.275742235886582	19.420038601241842
9	24.660624201808897	14.656338084725448	21.76928468983265	38.913753023633
10	16.73287888271141	27.060500594033133	33.74771618048481	22.458904342770648
11	33.20588463798024	22.549427833922127	23.265933923962013	20.978753604135623
12	20.915291137339935	26.846543914368443	30.989848624121496	21.248316324170123
13	30.872525989495088	21.00668756476096	26.1570115550574	21.963774890686555
14	20.754583570117298	22.83889350090218	27.443719616361967	28.962803312618558
15	23.251355888260665	29.13206565528271	25.12441524181644	22.49216321464019
16	22.200515206986285	28.085327774475168	26.463412185666563	23.250744832871987
17	21.8674900201561	26.88713545090214	27.040772234341485	24.204602294600278
18	22.824839226962556	25.770911843039073	29.059000889522057	22.34524804047631
19	22.98790372211296	25.26452151307789	29.868125517760326	21.87944924704882
20	23.68302287354907	25.039653130043938	29.712568274527985	21.56475572187901
21	22.731958807883313	25.691387348883822	29.96851318875763	21.60814065447524
22	21.76570565112753	26.05086250468112	29.65635117876949	22.52708066542186
23	22.452968376137765	26.153956278114006	30.357580884092393	21.035494461655837
24	22.502376568993828	26.25093949766009	28.496568051056297	22.75011588228978
25	22.564005869623475	26.298776405230985	29.206003357312895	21.931214367832645
26	22.16245518563426	26.89394435380456	29.25462590752637	21.688974553034807
27	21.50827674523966	26.939860801582462	29.034733261228794	22.517129191949085
28	21.75846028009033	26.61905672252586	29.28203610638998	22.340446890993828
29	22.052552509298952	26.579425415888664	28.88729432530319	22.480727749509192
30	21.912097063529686	26.729395866995937	29.99889137093768	21.359615698536697
31	22.342891112548546	26.474498476289742	29.866903406982964	21.315707004178748
32	21.867577313783052	26.371230115602952	29.219883043998607	22.54130952661539
33	21.66811137619276	26.361889697518855	29.68690394820345	22.283094978084936
34	21.222651997845592	26.515788361839064	30.074225770999135	22.187333869316202
35	21.08944192311352	27.933262276320992	29.21010615777974	21.767189642785752
36	22.458817049143693	26.280095569062787	29.17937880109187	22.081708580701648
37	21.786132359834806	26.59295592806656	29.236206952239037	22.3847047598596
38	21.594348261416478	25.635781308514012	30.336281239115575	22.43358919095394
39	21.01087765885476	25.621814328201342	30.936075749917723	22.431232263026175
40	20.405059887792774	26.041958554731792	30.635523792314494	22.91745776516094
41	21.217676261109204	25.34710128417655	30.621731399255737	22.813491055458517
42	20.202800554139944	26.793120214672488	30.190413588475145	22.813665642712422
43	20.49619443433293	26.468737096910765	29.69641895354146	23.338649515214843
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	82.0
1	159.0
2	236.0
3	623.0
4	1010.0
5	1010.0
6	1508.5
7	2007.0
8	2200.5
9	2394.0
10	3515.0
11	4636.0
12	4636.0
13	8573.5
14	12511.0
15	18293.0
16	24075.0
17	22181.0
18	20287.0
19	20287.0
20	22151.5
21	24016.0
22	19790.5
23	15565.0
24	17590.0
25	19615.0
26	19615.0
27	21635.0
28	23655.0
29	26332.5
30	29010.0
31	32434.0
32	35858.0
33	35858.0
34	39829.5
35	43801.0
36	47448.0
37	51095.0
38	54469.5
39	57844.0
40	57844.0
41	60092.0
42	62340.0
43	66127.5
44	69915.0
45	73263.5
46	76612.0
47	76612.0
48	93113.0
49	109614.0
50	104263.0
51	98912.0
52	91969.0
53	85026.0
54	85026.0
55	77782.0
56	70538.0
57	61877.5
58	53217.0
59	47906.0
60	42595.0
61	42595.0
62	38020.5
63	33446.0
64	28948.5
65	24451.0
66	20973.0
67	17495.0
68	17495.0
69	14982.0
70	12469.0
71	10400.5
72	8332.0
73	6894.0
74	5456.0
75	5456.0
76	4399.5
77	3343.0
78	2616.0
79	1889.0
80	1457.0
81	1025.0
82	1025.0
83	792.5
84	560.0
85	442.0
86	324.0
87	231.0
88	138.0
89	138.0
90	125.5
91	113.0
92	71.5
93	30.0
94	21.5
95	13.0
96	13.0
97	10.5
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1145559.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.84307047894904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.01800640617154	53.19617632635556
2	8.278920339853517	10.239877081343316
3	2.189951093418107	4.062998994471224
4	0.981522575743096	2.428014793134395
5	0.5403593942470095	1.6708742051190009
6	0.3767750258528361	1.3980554687114872
7	0.2580864103009569	1.1172599243330723
8	0.20728413379973656	1.0255269836596006
9	0.14706568631884565	0.8185494243646243
>10	0.8826553246110141	10.173567858084098
>50	0.07186881929241516	3.098852671204671
>100	0.04269776135231023	5.056934254942243
>500	0.0024035145193331713	1.051676796408494
>1k	0.0019793648982743763	2.1520552868309166
>5k	1.4138320701959832E-4	0.6624129871625167
>10k+	2.8276641403919664E-4	1.847166943874779
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10857	0.9477469078414992	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10269	0.8964182551924431	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7576	0.6613365178048446	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4537	0.3960511854911008	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2182	0.19047469401401412	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2112	0.18436414012722174	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2038	0.17790441173261262	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1998	0.17441266665444558	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1976	0.17249220686145367	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1680	0.1466532932830173	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1328	0.11592593659514701	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1249	0.10902974006576703	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1150	0.10038767099730349	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7458725390835392E-4
2	0.0	0.0	0.0	0.0	1.7458725390835392E-4
3	0.0	0.0	0.0	0.0	1.7458725390835392E-4
4	0.0	0.0	0.0	0.0	1.7458725390835392E-4
5	0.0	0.0	0.0	0.0	1.7458725390835392E-4
6	0.0	0.0	0.0	0.0	1.7458725390835392E-4
7	0.0	0.0	0.0	0.0	1.7458725390835392E-4
8	0.0	0.0	0.0	8.729362695417696E-5	1.7458725390835392E-4
9	0.0	0.0	0.0	2.6188088086253085E-4	1.7458725390835392E-4
10	0.0	0.0	0.0	4.364681347708848E-4	1.7458725390835392E-4
11	0.0	0.0	0.0	9.602298964959465E-4	1.7458725390835392E-4
12	0.0	0.0	0.0	0.0016585789121293622	1.7458725390835392E-4
13	0.0	0.0	0.0	0.0017458725390835392	1.7458725390835392E-4
14	0.0	0.0	0.0	0.001920459792991893	1.7458725390835392E-4
15	0.0	0.0	0.0	0.0034917450781670784	1.7458725390835392E-4
16	0.0	0.0	0.0	0.005063030363342264	1.7458725390835392E-4
17	0.0	0.0	0.0	0.006634315648517449	1.7458725390835392E-4
18	0.0	0.0	0.0	0.007856426425875926	1.7458725390835392E-4
19	0.0	0.0	0.0	0.00942771171105111	1.7458725390835392E-4
20	0.0	0.0	0.0	0.013006750416172367	1.7458725390835392E-4
21	0.0	0.0	0.0	0.0189427170490564	2.6188088086253085E-4
22	0.0	0.0	0.0	0.02985442041832852	2.6188088086253085E-4
23	0.0	0.0	0.0	0.03753625959029609	2.6188088086253085E-4
24	0.0	0.0	0.0	0.04967007373692669	2.6188088086253085E-4
25	0.0	0.0	0.0	0.057439206535848435	2.6188088086253085E-4
26	8.729362695417696E-5	0.0	0.0	0.06808902902425802	2.6188088086253085E-4
27	8.729362695417696E-5	0.0	0.0	0.12186190322803103	2.6188088086253085E-4
28	8.729362695417696E-5	0.0	0.0	0.24110499764743676	2.6188088086253085E-4
29	8.729362695417696E-5	0.0	0.0	0.39142462326252947	2.6188088086253085E-4
30	8.729362695417696E-5	0.0	0.0	0.6155946572808559	2.6188088086253085E-4
31	8.729362695417696E-5	0.0	0.0	0.9475723205875909	2.6188088086253085E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTTA	50	2.7207716E-7	29.599998	26
GTCGATT	45	1.3232081E-4	24.666668	18
CGAGTAT	40	0.0019311828	23.125002	26
GGTATCA	3645	0.0	21.51989	1
CTATCGG	65	6.902103E-5	19.923077	12
CGTTTAT	85	1.2453002E-6	19.588236	27
ATCGGTC	70	1.2191924E-4	18.5	14
TAAATCG	50	0.00703487	18.499998	5
ACGAGTC	50	0.00703487	18.499998	26
TAATACT	255	0.0	17.411764	4
GTCTAGG	75	2.0672582E-4	17.266666	2
GCTCTAT	75	2.0672582E-4	17.266666	1
ATGCTAG	180	1.0913936E-11	16.444445	15
TAAGCCG	70	0.0025924859	15.857143	36
AGTACCG	95	7.059645E-5	15.578948	5
AATACTG	275	0.0	15.472728	5
ACCGTTA	120	1.9353884E-6	15.416666	8
CTAATAC	245	0.0	15.10204	3
ACTGTAC	135	3.9717997E-7	15.074075	3
CTAGGCT	150	8.099596E-8	14.8	4
>>END_MODULE