FastQCFastQC Report
Wed 25 May 2016
SRR2088585_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088585_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372114
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT218231.5904655152560212No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT207061.5090582852445205No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT113040.8238382525067158No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82130.5985654253218027No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG43570.3175392132140624No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA42130.3070444584050596No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG41390.30165132051709986No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA39930.29101080522463874No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA39410.2872210326547211No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23650.17236177168952435No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23400.1705397656462947No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG22820.16631271162600192No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA18170.13242339922193053No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17950.13082003390388844No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC17620.12841498592682532No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC17140.12491673432382441No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA16020.1167541472501556No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA15810.11522366217384271No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG15720.11456773999828003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65600.024.1966481
GTTAGCC450.0038261820.5555553
CTCGACG656.903376E-519.92307718
ATACCGT851.2456694E-619.5882346
TTAGCCG500.0070355618.54
ATACCGC801.6170425E-518.56
GTATCAA90750.017.490912
GCAATAC852.7237395E-517.4117643
GATACTG4450.016.21348236
TACGTTA803.3831625E-416.187519
ATACACT1902.7284841E-1115.5789484
ATCACGT2650.015.35849124
ATGTACG855.365094E-415.2352934
GGCTAGC855.365094E-415.23529325
CTAGGAC1101.4521967E-515.1363643
TACCGTG1101.4521967E-515.1363647
ACGTTCG1353.9734732E-715.07407322
CTAATTC1252.959574E-614.7999993
ATACTGT4800.014.64583437
TCACGTA2800.014.53571525
Produced by FastQC (version 0.11.5)