FastQCFastQC Report
Wed 25 May 2016
SRR2088584_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088584_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5118352
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT473740.9255713557801417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT447540.8743830045295828No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT349760.6833449516563144No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197960.3867651150213975No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG113700.22214181439650887No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA104750.20465571730900883No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA100680.19670393908039152No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG94540.1847078903522071No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA89970.1757792351913272No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG77990.15237326389431596No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA61160.11949158635435782No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA54960.1073783123943019No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG54160.1058153093026818No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52330.1022399397306008No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT52170.10192733911227676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152100.021.5407621
GTATTGG25100.017.4681261
CGCGATA752.0690238E-417.26666614
TTAACGG9150.016.98360635
TAGACCG1005.885773E-616.655
GTATTAG14950.016.2107011
TAATACT14850.015.9461284
CTAATAC13750.015.6072723
ATTAGAG12600.015.2698423
TCTAATA14050.015.1423492
TAACGGC9850.015.0253836
CTCTAAT12700.015.0039371
ATTGGAC29250.014.4837613
GTATCAA227600.014.395212
TGCTAGA12150.014.31275716
AACGGCC10900.014.25688137
GGTAAAC11900.014.147058535
TGGACCC33050.014.1059015
TTGGACC35950.014.0486794
TATACTG6650.013.9097745
Produced by FastQC (version 0.11.5)