##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088584_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5118352
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71232635035652	31.0	31.0	34.0	30.0	34.0
2	31.890835761198137	31.0	31.0	34.0	30.0	34.0
3	31.977234664595166	31.0	31.0	34.0	30.0	34.0
4	35.66001967039391	37.0	35.0	37.0	33.0	37.0
5	35.56337938461442	37.0	35.0	37.0	33.0	37.0
6	35.58855076790342	37.0	35.0	37.0	33.0	37.0
7	35.45847198473258	37.0	35.0	37.0	33.0	37.0
8	35.47674974288599	37.0	35.0	37.0	33.0	37.0
9	37.087331625491956	39.0	37.0	39.0	33.0	39.0
10	36.95113388059281	39.0	37.0	39.0	33.0	39.0
11	36.99780691128707	39.0	37.0	39.0	33.0	39.0
12	36.90650975157629	39.0	37.0	39.0	33.0	39.0
13	36.94294628427275	39.0	37.0	39.0	33.0	39.0
14	38.002819266826506	40.0	37.0	41.0	33.0	41.0
15	37.95763304282316	40.0	37.0	41.0	33.0	41.0
16	37.863763570774346	40.0	37.0	41.0	32.0	41.0
17	37.88186588183071	40.0	37.0	41.0	33.0	41.0
18	37.85683301969071	40.0	37.0	41.0	32.0	41.0
19	37.874346078581546	40.0	37.0	41.0	32.0	41.0
20	37.75340382998277	40.0	37.0	41.0	32.0	41.0
21	37.653793056827666	40.0	37.0	41.0	32.0	41.0
22	37.5037250271181	39.0	36.0	41.0	32.0	41.0
23	37.37853942050097	39.0	36.0	41.0	31.0	41.0
24	37.24334277908202	39.0	36.0	41.0	31.0	41.0
25	37.052601892171545	39.0	36.0	41.0	31.0	41.0
26	37.2038064205041	39.0	36.0	41.0	31.0	41.0
27	37.13803837641491	39.0	36.0	41.0	31.0	41.0
28	37.01909051976105	39.0	36.0	41.0	31.0	41.0
29	36.886431999987494	39.0	35.0	41.0	30.0	41.0
30	36.776958091198104	39.0	35.0	41.0	30.0	41.0
31	36.608347569686494	39.0	35.0	40.0	30.0	41.0
32	36.49114500135981	39.0	35.0	40.0	30.0	41.0
33	36.295184270249486	38.0	35.0	40.0	30.0	41.0
34	36.160030416040165	38.0	35.0	40.0	30.0	41.0
35	35.98311761285664	38.0	35.0	40.0	29.0	41.0
36	35.849308722807656	38.0	35.0	40.0	28.0	41.0
37	35.69301857316574	38.0	35.0	40.0	27.0	41.0
38	35.614772684645374	38.0	35.0	40.0	27.0	41.0
39	35.49290650584407	38.0	35.0	40.0	26.0	41.0
40	35.32694859595432	38.0	34.0	40.0	25.0	41.0
41	35.21646088428463	38.0	34.0	40.0	24.0	41.0
42	35.01818202421404	38.0	34.0	40.0	24.0	41.0
43	34.6782673407378	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	7.0
14	12.0
15	26.0
16	62.0
17	138.0
18	375.0
19	812.0
20	1665.0
21	3476.0
22	6328.0
23	11031.0
24	18014.0
25	27772.0
26	41125.0
27	57392.0
28	79084.0
29	103838.0
30	131718.0
31	161864.0
32	199501.0
33	245815.0
34	304830.0
35	377992.0
36	491315.0
37	696446.0
38	975087.0
39	1182622.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.1276809410529	20.66497185031432	13.220778875700617	23.986568332932162
2	18.866502342941637	22.5860589502246	35.222528657661684	23.324910049172075
3	19.626922884553466	24.526351450623167	31.91820335920624	23.92852230561712
4	13.849145193609194	16.016522505681515	36.227031669568646	33.90730063114064
5	13.227323951146774	37.854997077184215	35.06659956173394	13.851079409935071
6	32.874604950968596	36.81294291600109	14.013651269002212	16.29880086402811
7	26.762266448263034	32.285255097734584	21.890444424299073	19.062034029703312
8	26.597760372870017	34.676415377449615	19.331065936848425	19.394758312831943
9	24.738685420619763	14.413584685070507	21.655779047630958	39.19195084667877
10	16.47958170911262	26.89609858798301	33.99623550705383	22.62808419585054
11	33.37494177813484	22.06112436190399	23.558051497825865	21.00588236213531
12	21.03589202149442	26.60561446340541	31.029010900383565	21.32948261471661
13	30.86149604403918	20.613119222749823	26.36006667771189	22.165318055499114
14	20.749667080341485	22.328495578264253	27.516278677199224	29.405558664195038
15	23.277570593034632	28.82902543631231	25.28274725927408	22.610656711378976
16	22.365128463224103	27.868794486975496	26.465139560546053	23.300937489254352
17	22.016715536563332	26.48979593431636	27.09145443689688	24.402034092223435
18	22.909815503115066	25.591225456943956	29.15614244584976	22.34281659409122
19	23.08897473249202	24.74823927701729	29.902652259946173	22.260133730544517
20	23.845702679299897	24.592368793705475	29.761669381082033	21.80025914591259
21	22.87441348308987	25.444420391563533	30.006044914456844	21.675121210889756
22	21.822160726733916	25.815672700900606	29.63508566819945	22.727080904166026
23	22.539168857476	25.86881480601569	30.520155706367984	21.07186063014033
24	22.758907554619142	25.93006498966855	28.34353713851646	22.967490317195846
25	22.736244009790653	25.948254438147277	29.173862993401002	22.14163855866107
26	22.385408428337872	26.585100047827893	29.251700547363686	21.777790976470552
27	21.556997252240564	26.741107293910225	28.90782032966861	22.794075124180598
28	21.873388153061768	26.250050797600476	29.31847985445315	22.5580811948846
29	22.237294347868218	26.307608386449388	28.791083536263233	22.664013729419157
30	22.23371897829614	26.299031406984124	30.01641934747747	21.450830267242267
31	22.568182102364197	26.065264757093686	29.88166894344117	21.484884197100943
32	22.133432792430064	25.867701166312905	29.22419169295117	22.77467434830586
33	21.837595382263668	25.96157903950334	29.77507213259268	22.425753445640314
34	21.505769825912715	26.299031406984124	30.11598264441367	22.07921612268949
35	21.25283685061129	27.85154284035174	29.0315124868317	21.864107822205273
36	22.86005339218561	26.044007915047658	29.02332625813934	22.072612434627395
37	21.956872055692926	26.233600190061175	29.15708024770473	22.65244750654117
38	21.83943191089632	25.213076396465112	30.24481317424046	22.70267851839811
39	21.333663647986693	25.234333238511148	30.767735396080614	22.664267717421545
40	20.59770410475872	25.817235703992225	30.50632312900715	23.07873706224191
41	21.56270221352498	24.857825331278505	30.57886600999697	23.000606445199548
42	20.35528232524844	26.53232915594707	30.14243647173934	22.96995204706515
43	20.73261080910418	26.062373201374196	29.52305742160758	23.681958567914048
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	313.0
1	671.0
2	1029.0
3	2802.5
4	4576.0
5	4576.0
6	6845.5
7	9115.0
8	9655.0
9	10195.0
10	15135.0
11	20075.0
12	20075.0
13	35920.5
14	51766.0
15	76752.5
16	101739.0
17	94977.0
18	88215.0
19	88215.0
20	97103.0
21	105991.0
22	87322.0
23	68653.0
24	77007.0
25	85361.0
26	85361.0
27	93258.0
28	101155.0
29	111748.0
30	122341.0
31	136886.0
32	151431.0
33	151431.0
34	170656.5
35	189882.0
36	209012.0
37	228142.0
38	242940.0
39	257738.0
40	257738.0
41	268479.0
42	279220.0
43	298908.0
44	318596.0
45	331366.5
46	344137.0
47	344137.0
48	419861.0
49	495585.0
50	474097.0
51	452609.0
52	420576.5
53	388544.0
54	388544.0
55	352757.5
56	316971.0
57	274225.5
58	231480.0
59	208605.0
60	185730.0
61	185730.0
62	166952.0
63	148174.0
64	130286.0
65	112398.0
66	97011.5
67	81625.0
68	81625.0
69	70769.0
70	59913.0
71	51064.5
72	42216.0
73	34578.5
74	26941.0
75	26941.0
76	21346.5
77	15752.0
78	12499.5
79	9247.0
80	7395.0
81	5543.0
82	5543.0
83	4144.0
84	2745.0
85	2146.5
86	1548.0
87	1174.5
88	801.0
89	801.0
90	663.5
91	526.0
92	360.5
93	195.0
94	150.0
95	105.0
96	105.0
97	63.0
98	21.0
99	17.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5118352.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.46813714424445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.69141149643104	33.3363279271255
2	12.337549673810313	10.725806024902303
3	4.337500393015876	5.656291858404849
4	2.016892568663509	3.5068225111949154
5	1.083130360783574	2.3540829533817687
6	0.66235682977	1.72748505089216
7	0.4566332504294056	1.389429772800127
8	0.3311414286841336	1.1515280828946368
9	0.23795647757718255	0.9309172321517671
>10	1.5628405201297226	13.156600428699589
>50	0.16081128725260152	4.862094093945249
>100	0.10476901380014815	8.867579523199867
>500	0.010646104624370477	3.2096569413681246
>1k	0.0056388253795789	4.465400041990627
>5k	4.0599542732963503E-4	1.1536801469777223
>10k+	3.1577422125638274E-4	3.506297410071003
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	47374	0.9255713557801417	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	44754	0.8743830045295828	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	34976	0.6833449516563144	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19796	0.3867651150213975	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11370	0.22214181439650887	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10475	0.20465571730900883	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10068	0.19670393908039152	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9454	0.1847078903522071	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	8997	0.1757792351913272	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7799	0.15237326389431596	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6116	0.11949158635435782	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5496	0.1073783123943019	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	5416	0.1058153093026818	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5233	0.1022399397306008	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5217	0.10192733911227676	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.9537538645251442E-5	0.0	0.0
2	1.9537538645251442E-5	0.0	1.9537538645251442E-5	1.9537538645251442E-5	0.0
3	1.9537538645251442E-5	0.0	1.9537538645251442E-5	1.9537538645251442E-5	0.0
4	1.9537538645251442E-5	0.0	1.9537538645251442E-5	1.9537538645251442E-5	0.0
5	1.9537538645251442E-5	0.0	1.9537538645251442E-5	1.9537538645251442E-5	0.0
6	1.9537538645251442E-5	0.0	1.9537538645251442E-5	1.9537538645251442E-5	0.0
7	1.9537538645251442E-5	0.0	5.861261593575432E-5	1.9537538645251442E-5	0.0
8	3.9075077290502884E-5	0.0	5.861261593575432E-5	3.9075077290502884E-5	0.0
9	3.9075077290502884E-5	0.0	9.76876932262572E-5	1.7583784780726296E-4	0.0
10	1.3676277051676009E-4	0.0	9.76876932262572E-5	2.3445046374301727E-4	0.0
11	1.953753864525144E-4	0.0	1.1722523187150864E-4	4.298258501955317E-4	0.0
12	1.953753864525144E-4	0.0	1.1722523187150864E-4	8.205766231005605E-4	0.0
13	1.953753864525144E-4	0.0	1.1722523187150864E-4	9.76876932262572E-4	0.0
14	2.3445046374301727E-4	0.0	1.1722523187150864E-4	0.001289477550586595	0.0
15	2.930630796787716E-4	0.0	1.1722523187150864E-4	0.0016606907848463724	0.0
16	3.321381569692745E-4	0.0	1.3676277051676009E-4	0.0024617298693016816	0.0
17	3.321381569692745E-4	0.0	1.3676277051676009E-4	0.003555832033435762	0.0
18	3.321381569692745E-4	0.0	1.3676277051676009E-4	0.004141958192793305	0.0
19	3.321381569692745E-4	0.0	1.3676277051676009E-4	0.0054314357433799	0.0
20	3.321381569692745E-4	0.0	1.3676277051676009E-4	0.008791892390363149	0.0
21	3.516756956145259E-4	0.0	1.3676277051676009E-4	0.014633616445293329	0.0
22	3.516756956145259E-4	0.0	1.3676277051676009E-4	0.024070247610949773	0.0
23	3.516756956145259E-4	0.0	1.3676277051676009E-4	0.030595785518463755	0.0
24	3.516756956145259E-4	0.0	1.3676277051676009E-4	0.04116559392554479	0.0
25	3.516756956145259E-4	0.0	1.3676277051676009E-4	0.04989887369997218	0.0
26	3.712132342597774E-4	0.0	1.3676277051676009E-4	0.060859432879958236	0.0
27	3.712132342597774E-4	0.0	1.3676277051676009E-4	0.11081691919586617	0.0
28	3.712132342597774E-4	0.0	1.3676277051676009E-4	0.21958239683398093	0.0
29	3.712132342597774E-4	0.0	1.3676277051676009E-4	0.3638280446518723	0.0
30	3.712132342597774E-4	0.0	1.3676277051676009E-4	0.5542604338271381	0.0
31	4.298258501955317E-4	0.0	1.3676277051676009E-4	0.8509965707712169	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15210	0.0	21.540762	1
GTATTGG	2510	0.0	17.468126	1
CGCGATA	75	2.0690238E-4	17.266666	14
TTAACGG	915	0.0	16.983606	35
TAGACCG	100	5.885773E-6	16.65	5
GTATTAG	1495	0.0	16.210701	1
TAATACT	1485	0.0	15.946128	4
CTAATAC	1375	0.0	15.607272	3
ATTAGAG	1260	0.0	15.269842	3
TCTAATA	1405	0.0	15.142349	2
TAACGGC	985	0.0	15.02538	36
CTCTAAT	1270	0.0	15.003937	1
ATTGGAC	2925	0.0	14.483761	3
GTATCAA	22760	0.0	14.39521	2
TGCTAGA	1215	0.0	14.312757	16
AACGGCC	1090	0.0	14.256881	37
GGTAAAC	1190	0.0	14.1470585	35
TGGACCC	3305	0.0	14.105901	5
TTGGACC	3595	0.0	14.048679	4
TATACTG	665	0.0	13.909774	5
>>END_MODULE