FastQCFastQC Report
Wed 25 May 2016
SRR2088583_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088583_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4027921
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT451211.1202056842723578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT397480.986811806884991No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT291990.7249149126807601No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168810.4190995801556187No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG90120.22373825107294804No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA86030.21358412938088903No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA85970.21343516916046765No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG79970.1985391471183273No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA72260.17939775879417694No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG57800.14349834567261868No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46190.11467454302107713No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA45460.11286219367261671No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42040.10437146110859673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129050.023.1518781
TTAACGG5300.015.70754735
GTATTGG17450.015.6905451
GTATCAA192400.015.5096152
GTATTAG10100.015.202971
TTGGACC25350.014.6686394
CTAATAC10400.014.4086543
TAATACT10700.014.1775694
TGGACCC24050.013.9230775
ATACTGG10200.013.60294156
GGACCGT7500.013.5666666
ATTGGAC20600.013.560683
TAACGGC6050.013.45454536
ATTAGAG8700.013.3965513
TCTATAC3600.013.3611113
ACCGTTA5550.013.3333338
GACCGTT5700.013.3070177
CTCTAAT8650.013.2601151
TAAACGT2102.046363E-913.2142854
GGACCCT26450.013.1493396
Produced by FastQC (version 0.11.5)