##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088583_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4027921
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.356209319894806	31.0	31.0	34.0	30.0	34.0
2	31.550250116623438	31.0	31.0	34.0	30.0	34.0
3	31.613129949668824	31.0	31.0	34.0	30.0	34.0
4	35.35575896349506	37.0	35.0	37.0	33.0	37.0
5	35.23766255594387	37.0	35.0	37.0	33.0	37.0
6	35.29675954419166	37.0	35.0	37.0	32.0	37.0
7	35.1646422558933	37.0	35.0	37.0	32.0	37.0
8	35.18941930589006	37.0	35.0	37.0	32.0	37.0
9	36.741899853547274	39.0	37.0	39.0	32.0	39.0
10	36.54824511205657	38.0	35.0	39.0	32.0	39.0
11	36.6216167099603	38.0	35.0	39.0	32.0	39.0
12	36.49783250465935	38.0	35.0	39.0	32.0	39.0
13	36.56683708543439	38.0	35.0	39.0	32.0	39.0
14	37.515870594284245	39.0	36.0	41.0	32.0	41.0
15	37.47028280842648	39.0	36.0	41.0	32.0	41.0
16	37.3470768170478	39.0	36.0	41.0	32.0	41.0
17	37.378115409909974	39.0	36.0	40.0	32.0	41.0
18	37.34850757996495	39.0	36.0	40.0	32.0	41.0
19	37.377490273518276	39.0	36.0	40.0	32.0	41.0
20	37.2515277732607	39.0	36.0	40.0	31.0	41.0
21	37.14254723466523	39.0	36.0	40.0	31.0	41.0
22	36.97970590783682	39.0	36.0	40.0	31.0	41.0
23	36.83906213652155	39.0	36.0	40.0	30.0	41.0
24	36.69617303815045	39.0	35.0	40.0	30.0	41.0
25	36.48515176936191	38.0	35.0	40.0	30.0	41.0
26	36.62802199943842	39.0	35.0	40.0	30.0	41.0
27	36.541007383213326	39.0	35.0	40.0	30.0	41.0
28	36.41523331763458	39.0	35.0	40.0	30.0	41.0
29	36.25920418995308	38.0	35.0	40.0	30.0	41.0
30	36.153349829850185	38.0	35.0	40.0	30.0	41.0
31	35.967191014918114	38.0	35.0	40.0	29.0	41.0
32	35.84459948444868	38.0	35.0	40.0	29.0	41.0
33	35.63141705112886	38.0	34.0	40.0	27.0	41.0
34	35.48909002932282	38.0	34.0	40.0	27.0	41.0
35	35.29848996541889	38.0	34.0	40.0	26.0	41.0
36	35.162631541184645	38.0	34.0	40.0	25.0	41.0
37	35.00269320078522	38.0	34.0	40.0	25.0	41.0
38	34.917701216086414	38.0	33.0	40.0	25.0	41.0
39	34.76967745891739	38.0	33.0	40.0	24.0	41.0
40	34.58872976902973	38.0	33.0	40.0	23.0	41.0
41	34.46589170939549	38.0	33.0	40.0	23.0	41.0
42	34.239471181286824	38.0	33.0	40.0	22.0	41.0
43	33.87708919812479	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	5.0
14	14.0
15	34.0
16	96.0
17	206.0
18	498.0
19	1028.0
20	2135.0
21	4090.0
22	7412.0
23	12272.0
24	19512.0
25	29617.0
26	42012.0
27	57971.0
28	76904.0
29	98577.0
30	123195.0
31	150474.0
32	181784.0
33	220603.0
34	269372.0
35	329126.0
36	425065.0
37	586578.0
38	790997.0
39	598341.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.569142244845416	22.29331210815704	14.262221130950682	22.875324516046863
2	19.8404834652914	23.930583544215487	33.78708271587253	22.441850274620577
3	20.842762308396814	25.1082133934603	31.960035958997207	22.08898833914568
4	14.872411847203557	17.14884676238685	35.690719852747854	32.288021537661734
5	13.0485925617707	38.799345866018726	34.57788769938636	13.574173872824218
6	32.51694856974603	37.43045109375283	13.72263755917755	16.329962777323587
7	26.251855485745622	32.68207097408315	21.759786252014376	19.30628728815684
8	27.09405174530484	34.77386969605411	18.99654933649394	19.135529222147106
9	24.7971347998136	14.278929502341281	21.526862120682107	39.397073577163006
10	16.63098655609184	27.484352349512314	33.504207257292286	22.38045383710356
11	33.717021758867666	22.326530237311008	23.206115512195993	20.750332491625333
12	20.736429537719335	27.05226840347663	30.985140969745935	21.226161089058103
13	31.410000345091177	20.751251079651265	26.266950121414993	21.571798453842568
14	20.60139709790733	22.45925379370648	27.723756250432917	29.215592857953272
15	23.43534046472113	29.23014130614776	25.032119547528364	22.302398681602742
16	22.165826986179717	27.914574292792732	26.563405786756988	23.35619293427056
17	22.024016856338545	26.627185587800756	27.217316327703543	24.131481228157156
18	22.84642623328511	25.447867522724504	29.341240803878726	22.364465440111662
19	23.05750286562224	24.876381637077788	30.17588974560325	21.89022575169672
20	23.76791898351532	24.526151332163664	30.053047217162405	21.652882467158616
21	22.89081637897069	25.34458843656566	30.321473534361772	21.443121650101876
22	21.74367372150546	25.74427353465969	29.960046386212642	22.552006357622208
23	22.441254433738894	25.88444510207623	30.747921818724844	20.926378645460026
24	22.525143864539547	25.88481750262729	28.710493577207696	22.87954505562547
25	22.605234809719455	25.835834416812048	29.607854771729635	21.951076001738862
26	22.42961070984262	26.441854246893126	29.61361456691926	21.514920476344994
27	21.656283725524904	26.496274380753743	29.287639951230425	22.559801942490928
28	21.84218608061082	26.30999962511678	29.554750453149403	22.293063841123
29	22.084122305278576	26.266106013499275	29.116360524449213	22.533411156772935
30	21.990972514108396	26.359528898406897	30.28261974353519	21.36687884394952
31	22.430181724020905	26.031046785674295	30.17268710086419	21.366084389440605
32	22.099912088643247	25.832954519217232	29.359240163846312	22.707893228293205
33	21.92168118490904	26.036856234270733	29.871092307917657	22.170370272902574
34	21.40903458632878	26.409579532468484	30.249674708118658	21.93171117308408
35	21.134029192727464	27.9352301100245	29.36619164079931	21.564549056448723
36	22.767874543716225	26.067790306711576	29.31065430528553	21.853680844286668
37	21.87163055084745	26.527978081000096	29.265916585752304	22.33447478240015
38	21.799856551307734	25.31879349172936	30.46301057046551	22.4183393864974
39	21.26978160693817	25.31874383832255	30.948074701564405	22.463399853174877
40	20.56145093213099	25.977917640390665	30.58155807921754	22.879073348260803
41	21.544091852844186	24.897956042335487	30.70757842569405	22.850373679126278
42	20.52684250758642	26.53932388445553	30.211242971249934	22.72259063670812
43	20.810909647930036	26.218711836701864	29.642686636604836	23.32769187876326
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	248.0
1	472.0
2	696.0
3	1549.0
4	2402.0
5	2402.0
6	4253.5
7	6105.0
8	6977.0
9	7849.0
10	11584.5
11	15320.0
12	15320.0
13	28910.0
14	42500.0
15	66843.5
16	91187.0
17	82617.0
18	74047.0
19	74047.0
20	81159.5
21	88272.0
22	71142.0
23	54012.0
24	60188.5
25	66365.0
26	66365.0
27	72955.0
28	79545.0
29	88669.5
30	97794.0
31	108415.5
32	119037.0
33	119037.0
34	133097.0
35	147157.0
36	163444.5
37	179732.0
38	190822.0
39	201912.0
40	201912.0
41	210967.5
42	220023.0
43	234042.5
44	248062.0
45	260082.0
46	272102.0
47	272102.0
48	333630.0
49	395158.0
50	377620.0
51	360082.0
52	336677.0
53	313272.0
54	313272.0
55	280837.0
56	248402.0
57	216321.0
58	184240.0
59	164068.0
60	143896.0
61	143896.0
62	128281.0
63	112666.0
64	97717.0
65	82768.0
66	70555.0
67	58342.0
68	58342.0
69	50217.0
70	42092.0
71	35493.0
72	28894.0
73	23889.5
74	18885.0
75	18885.0
76	14993.5
77	11102.0
78	8703.5
79	6305.0
80	4905.0
81	3505.0
82	3505.0
83	2659.5
84	1814.0
85	1405.5
86	997.0
87	760.0
88	523.0
89	523.0
90	477.0
91	431.0
92	269.5
93	108.0
94	79.5
95	51.0
96	51.0
97	32.5
98	14.0
99	10.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4027921.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.58947975752643
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.99023471312161	36.335244614517144
2	11.875687937593622	11.065642455504378
3	4.148125927439759	5.797770867843756
4	1.8266787447817745	3.404160496140571
5	1.0253871706283824	2.388612741480916
6	0.6139068057850109	1.716095922067709
7	0.4097448106607302	1.3362858293420714
8	0.2929084521207336	1.0917161920709855
9	0.22032930425230332	0.9238524894407312
>10	1.3475904050971033	11.984464259264955
>50	0.14123257896359234	4.555379859965467
>100	0.09573270237944685	8.746220376756844
>500	0.007635124243035453	2.508099588986402
>1k	0.00427139816256403	3.714575405501945
>5k	3.203548621923022E-4	1.1744862167227468
>10k+	2.135699081282015E-4	3.2573926843932433
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	45121	1.1202056842723578	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	39748	0.986811806884991	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	29199	0.7249149126807601	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16881	0.4190995801556187	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9012	0.22373825107294804	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8603	0.21358412938088903	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8597	0.21343516916046765	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7997	0.1985391471183273	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7226	0.17939775879417694	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5780	0.14349834567261868	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4619	0.11467454302107713	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4546	0.11286219367261671	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4204	0.10437146110859673	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	4.96534068071345E-5	0.0	0.0	0.0	0.0
7	7.448011021070175E-5	0.0	0.0	2.482670340356725E-5	0.0
8	7.448011021070175E-5	0.0	0.0	2.482670340356725E-5	0.0
9	7.448011021070175E-5	0.0	0.0	9.9306813614269E-5	0.0
10	1.2413351701783624E-4	0.0	0.0	1.7378692382497075E-4	0.0
11	1.2413351701783624E-4	0.0	0.0	3.2274714424637425E-4	0.0
12	1.2413351701783624E-4	0.0	0.0	5.710141782820468E-4	0.0
13	1.2413351701783624E-4	0.0	0.0	6.95147695299883E-4	0.0
14	1.7378692382497075E-4	0.0	0.0	7.94454508914152E-4	0.0
15	1.7378692382497075E-4	0.0	0.0	0.0010675482463533917	0.0
16	1.7378692382497075E-4	0.0	0.0	0.0014399487974069004	0.0
17	1.7378692382497075E-4	0.0	0.0	0.0022592300097246196	0.0
18	1.7378692382497075E-4	0.0	0.0	0.002681283967585263	0.0
19	1.7378692382497075E-4	0.0	0.0	0.0035253918833065495	0.0
20	1.98613627228538E-4	0.0	0.0	0.00595840881685614	0.0
21	1.98613627228538E-4	0.0	0.0	0.010377562022691111	0.0
22	1.98613627228538E-4	0.0	0.0	0.01698146512804	0.0
23	1.98613627228538E-4	0.0	0.0	0.021251658113453566	0.0
24	2.2344033063210526E-4	0.0	0.0	0.028550708914102337	0.0
25	2.2344033063210526E-4	0.0	0.0	0.03356570300162292	0.0
26	2.2344033063210526E-4	0.0	0.0	0.04190747534522152	0.0
27	2.2344033063210526E-4	0.0	0.0	0.08624796762399263	0.0
28	2.2344033063210526E-4	0.0	0.0	0.1828486705672728	0.0
29	2.2344033063210526E-4	0.0	0.0	0.3044250371345416	0.0
30	2.2344033063210526E-4	0.0	0.0	0.4668413308006786	0.0
31	2.2344033063210526E-4	0.0	0.0	0.7125263876823801	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12905	0.0	23.151878	1
TTAACGG	530	0.0	15.707547	35
GTATTGG	1745	0.0	15.690545	1
GTATCAA	19240	0.0	15.509615	2
GTATTAG	1010	0.0	15.20297	1
TTGGACC	2535	0.0	14.668639	4
CTAATAC	1040	0.0	14.408654	3
TAATACT	1070	0.0	14.177569	4
TGGACCC	2405	0.0	13.923077	5
ATACTGG	1020	0.0	13.6029415	6
GGACCGT	750	0.0	13.566666	6
ATTGGAC	2060	0.0	13.56068	3
TAACGGC	605	0.0	13.454545	36
ATTAGAG	870	0.0	13.396551	3
TCTATAC	360	0.0	13.361111	3
ACCGTTA	555	0.0	13.333333	8
GACCGTT	570	0.0	13.307017	7
CTCTAAT	865	0.0	13.260115	1
TAAACGT	210	2.046363E-9	13.214285	4
GGACCCT	2645	0.0	13.149339	6
>>END_MODULE