FastQCFastQC Report
Wed 25 May 2016
SRR2088582_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088582_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3766574
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT373610.9919093584780228No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT354440.9410143010597959No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT275730.7320445582643538No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147690.39210699165873286No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG91390.2426342878169923No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA88810.23578456177948448No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA88690.23546596987076318No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG77900.20681924741157348No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA73880.1961464184694101No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG63910.16967674071981595No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA51970.13797684580204717No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA43350.11509132702556754No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG42650.11323287422469332No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC41500.11017970176611423No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40010.10622385223282485No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38140.10125912832191801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120700.021.6267621
GTATTGG22000.017.9113641
TTAACGG4000.016.65000235
ATTGGAC24850.016.1549283
TTGGACC29800.015.2097314
GTCTAGA2600.014.9423071
TGGACCC30300.014.6534665
TAATACT10000.014.4299994
TATTGGA27900.014.2562732
GTACTAT3900.014.230771
GTATCAA183350.014.16634752
TCGGGTA3550.014.07042225
GGACCCT31700.013.8895896
TAACGGC4950.013.82828336
CTAATAC9000.013.77222253
GTATTAG8200.013.7621951
GGACCGT8350.013.73652656
CGAACTA6300.013.50793724
ACCGTTA6050.013.4545458
AACGGCC5450.013.23853237
Produced by FastQC (version 0.11.5)