##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088582_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3766574
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.730737800452083	31.0	31.0	34.0	30.0	34.0
2	31.910975331959495	31.0	31.0	34.0	30.0	34.0
3	31.99201953817979	33.0	31.0	34.0	30.0	34.0
4	35.667962450757635	37.0	35.0	37.0	33.0	37.0
5	35.57928318944484	37.0	35.0	37.0	33.0	37.0
6	35.61425157185283	37.0	35.0	37.0	33.0	37.0
7	35.484998568991344	37.0	35.0	37.0	33.0	37.0
8	35.50750682184925	37.0	35.0	37.0	33.0	37.0
9	37.11719721954221	39.0	37.0	39.0	33.0	39.0
10	36.98346959332274	39.0	37.0	39.0	33.0	39.0
11	37.030826953087875	39.0	37.0	39.0	33.0	39.0
12	36.93255860630907	39.0	37.0	39.0	33.0	39.0
13	36.96982483285872	39.0	37.0	39.0	33.0	39.0
14	38.02050855764416	40.0	37.0	41.0	33.0	41.0
15	37.98360818080303	40.0	37.0	41.0	33.0	41.0
16	37.87920508132855	40.0	37.0	41.0	32.0	41.0
17	37.90229662287267	40.0	37.0	41.0	33.0	41.0
18	37.879357474458224	40.0	37.0	41.0	33.0	41.0
19	37.90174067999195	40.0	37.0	41.0	32.0	41.0
20	37.78284085219088	40.0	37.0	41.0	32.0	41.0
21	37.683893108166735	40.0	37.0	41.0	32.0	41.0
22	37.53044145687832	39.0	36.0	41.0	32.0	41.0
23	37.39688268437047	39.0	36.0	41.0	32.0	41.0
24	37.263364532330975	39.0	36.0	41.0	31.0	41.0
25	37.06786060754415	39.0	36.0	41.0	31.0	41.0
26	37.226562122501775	39.0	36.0	41.0	31.0	41.0
27	37.163392249827034	39.0	36.0	41.0	31.0	41.0
28	37.050145304459704	39.0	36.0	41.0	31.0	41.0
29	36.91165472920484	39.0	36.0	41.0	30.0	41.0
30	36.79346217544113	39.0	35.0	41.0	30.0	41.0
31	36.613415002599176	39.0	35.0	40.0	30.0	41.0
32	36.49822862898751	39.0	35.0	40.0	30.0	41.0
33	36.29576878086027	38.0	35.0	40.0	30.0	41.0
34	36.15464796390566	38.0	35.0	40.0	30.0	41.0
35	35.96572402400696	38.0	35.0	40.0	29.0	41.0
36	35.831877722301485	38.0	35.0	40.0	28.0	41.0
37	35.6743613692443	38.0	35.0	40.0	27.0	41.0
38	35.59454719328493	38.0	35.0	40.0	26.0	41.0
39	35.460290173510465	38.0	34.0	40.0	26.0	41.0
40	35.293701915852445	38.0	34.0	40.0	25.0	41.0
41	35.1731703664922	38.0	34.0	40.0	24.0	41.0
42	34.95795091242068	38.0	34.0	40.0	24.0	41.0
43	34.610366343525975	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	7.0
14	10.0
15	15.0
16	36.0
17	107.0
18	264.0
19	597.0
20	1199.0
21	2435.0
22	4536.0
23	8092.0
24	13056.0
25	20313.0
26	29695.0
27	42278.0
28	57193.0
29	75045.0
30	95588.0
31	117985.0
32	145958.0
33	180938.0
34	224407.0
35	278755.0
36	363615.0
37	520466.0
38	725947.0
39	858031.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.068654432383376	21.02303047809495	13.45541067293514	23.45290441658653
2	18.9457050359292	22.634786944315973	35.33009042169356	23.089417598061264
3	19.879975808254397	24.755547083370725	31.98346826585645	23.381008842518426
4	13.81616291091055	16.064227066825186	36.271370216010624	33.84823980625364
5	13.147916382367637	37.964633112212844	35.04882686494411	13.838623640475403
6	32.773390354205176	36.77333831752675	13.844968929324104	16.608302398943977
7	26.432296298970897	32.339999161041305	21.880573699069764	19.347130840918034
8	26.656027466870423	34.68382673485241	19.183056007926567	19.477089790350593
9	24.464168233519374	14.505569252057708	21.87486559403851	39.15539692038441
10	16.651445053249983	26.807040031604316	33.8251949915228	22.716319923622898
11	33.249154271228974	22.245175589275558	23.51481744418137	20.990852695314096
12	20.94983398706623	26.661018740107057	31.144137882330202	21.24500939049651
13	31.24343236054834	20.709376743958835	26.355064310431707	21.692126585061118
14	20.64889737995324	22.66858423596616	27.48179114495029	29.20072723913031
15	23.21775703862449	28.973252616303302	25.337056964764265	22.47193338030794
16	22.25075094767818	27.899305841329546	26.591406408051455	23.258536802940817
17	21.909326618831862	26.399242388441063	27.05501073389239	24.636420258834686
18	23.090293725810245	25.33915436149668	29.188169408061544	22.38238250463153
19	23.06719581242795	24.698466033058157	30.06565117265717	22.168686981856723
20	23.981474942480887	24.343236054833916	29.887345901076152	21.78794310160905
21	22.894970336438366	25.165627968546485	30.244726374684262	21.694675320330887
22	21.800235439420547	25.54684973665724	29.897169151595055	22.75574567232716
23	22.495190589644594	25.818582085470776	30.717994655089743	20.968232669794883
24	22.68634573487737	25.851954587909333	28.528206269145386	22.933493408067914
25	22.657991055001176	25.75796997483655	29.42326368737213	22.160775282790144
26	22.396400548615265	26.341577252962505	29.562727295414877	21.69929490300735
27	21.587017804508818	26.450987024282547	29.19464744353888	22.76734772766976
28	21.88957392049114	26.17649354559342	29.37332440568007	22.56060812823537
29	22.239201990987034	26.15387352007421	28.86365699970318	22.743267489235574
30	22.01658589476803	26.316196097567712	30.248788421520455	21.4184295861438
31	22.583148505777398	25.8747339093829	30.165317341435475	21.37680024340422
32	22.217564290519714	25.73033212675498	29.193373075903988	22.858730506821317
33	21.918990573396407	25.816033350201007	29.818317654186536	22.44665842221605
34	21.451430398022183	26.205140268052613	30.196459700512985	22.14696963341222
35	21.078969907401262	27.995122357877477	29.25945434763793	21.66645338708333
36	22.77140977450596	26.034587399583813	29.198364349140626	21.995638476769606
37	21.91445063869713	26.496731512509776	29.167248539388847	22.421569309404248
38	21.798430085271125	25.18649573856773	30.40245591882703	22.61261825733412
39	21.288364439408333	25.096068735142335	31.031170501362777	22.584396324086555
40	20.48238000899491	25.845131411197546	30.676896298864698	22.99559228094284
41	21.71036597183541	24.73393593222913	30.653559441550865	22.902138654384594
42	20.340686257591116	26.553361224284988	30.272178377485748	22.83377414063815
43	20.798927619635244	26.24597313102039	29.530098174096675	23.425001075247692
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	188.0
1	430.5
2	673.0
3	1894.5
4	3116.0
5	3116.0
6	4809.0
7	6502.0
8	7101.5
9	7701.0
10	11271.5
11	14842.0
12	14842.0
13	26792.5
14	38743.0
15	58646.5
16	78550.0
17	73289.5
18	68029.0
19	68029.0
20	73452.0
21	78875.0
22	64359.0
23	49843.0
24	56391.5
25	62940.0
26	62940.0
27	69333.0
28	75726.0
29	84015.5
30	92305.0
31	101243.0
32	110181.0
33	110181.0
34	121850.5
35	133520.0
36	147637.0
37	161754.0
38	172645.0
39	183536.0
40	183536.0
41	190277.0
42	197018.0
43	210393.0
44	223768.0
45	236931.5
46	250095.0
47	250095.0
48	313768.5
49	377442.0
50	361105.0
51	344768.0
52	323686.5
53	302605.0
54	302605.0
55	273489.0
56	244373.0
57	210357.0
58	176341.0
59	157181.0
60	138021.0
61	138021.0
62	121936.0
63	105851.0
64	92309.0
65	78767.0
66	67088.0
67	55409.0
68	55409.0
69	47274.5
70	39140.0
71	32809.5
72	26479.0
73	21612.0
74	16745.0
75	16745.0
76	13478.0
77	10211.0
78	7970.0
79	5729.0
80	4451.0
81	3173.0
82	3173.0
83	2430.5
84	1688.0
85	1307.5
86	927.0
87	722.5
88	518.0
89	518.0
90	396.5
91	275.0
92	197.5
93	120.0
94	88.5
95	57.0
96	57.0
97	37.5
98	18.0
99	15.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3766574.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.03669101990231
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.84242366368034	36.61450030108864
2	12.350724212459436	11.618743973069616
3	4.107208609867473	5.795685070098563
4	1.819858206862346	3.424004327048704
5	0.9552205108341032	2.246520601198848
6	0.5933967436841658	1.6746851570932964
7	0.3805392545041462	1.2529515134538844
8	0.27423343959748425	1.0319226852537526
9	0.20168783434040058	0.8538055511708397
>10	1.2287925109220874	11.166275823250192
>50	0.13836187927600843	4.500792115154768
>100	0.09477421939529392	8.79333960937426
>500	0.0074072469483342914	2.4560413570693025
>1k	0.004749685060888259	4.0812122576752925
>5k	3.9580708840735487E-4	1.4270279810072843
>10k+	2.2617547908991705E-4	3.062491676992746
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	37361	0.9919093584780228	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	35444	0.9410143010597959	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	27573	0.7320445582643538	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14769	0.39210699165873286	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9139	0.2426342878169923	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8881	0.23578456177948448	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8869	0.23546596987076318	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7790	0.20681924741157348	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7388	0.1961464184694101	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6391	0.16967674071981595	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5197	0.13797684580204717	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4335	0.11509132702556754	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4265	0.11323287422469332	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4150	0.11017970176611423	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4001	0.10622385223282485	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3814	0.10125912832191801	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.3098651453549036E-5
2	0.0	0.0	0.0	2.6549325726774518E-5	5.3098651453549036E-5
3	0.0	0.0	0.0	2.6549325726774518E-5	5.3098651453549036E-5
4	0.0	0.0	0.0	5.3098651453549036E-5	5.3098651453549036E-5
5	0.0	0.0	0.0	1.0619730290709807E-4	7.964797718032355E-5
6	0.0	0.0	0.0	1.327466286338726E-4	7.964797718032355E-5
7	2.6549325726774518E-5	0.0	0.0	1.327466286338726E-4	7.964797718032355E-5
8	1.327466286338726E-4	0.0	0.0	1.592959543606471E-4	7.964797718032355E-5
9	1.327466286338726E-4	0.0	0.0	4.247892116283923E-4	7.964797718032355E-5
10	1.592959543606471E-4	0.0	0.0	5.840851659890394E-4	7.964797718032355E-5
11	1.8584528008742162E-4	0.0	0.0	9.026770747103336E-4	7.964797718032355E-5
12	1.8584528008742162E-4	0.0	0.0	0.0013009169606119514	7.964797718032355E-5
13	1.8584528008742162E-4	0.0	0.0	0.0014071142635190494	7.964797718032355E-5
14	1.8584528008742162E-4	0.0	0.0	0.0015664102178796966	7.964797718032355E-5
15	1.8584528008742162E-4	0.0	0.0	0.002044298080961638	7.964797718032355E-5
16	2.3894393154097064E-4	0.0	0.0	0.002628383246950677	7.964797718032355E-5
17	2.3894393154097064E-4	0.0	0.0	0.003796553578928756	7.964797718032355E-5
18	2.3894393154097064E-4	0.0	0.0	0.004300990767737472	7.964797718032355E-5
19	2.920425829945197E-4	0.0	0.0	0.005283315819628129	7.964797718032355E-5
20	2.920425829945197E-4	0.0	0.0	0.008655080186928493	7.964797718032355E-5
21	2.920425829945197E-4	0.0	0.0	0.014655227801179534	7.964797718032355E-5
22	2.920425829945197E-4	0.0	0.0	0.02492981685744127	7.964797718032355E-5
23	2.920425829945197E-4	0.0	0.0	0.03260257199247911	1.0619730290709807E-4
24	2.920425829945197E-4	0.0	0.0	0.04447012059234732	1.0619730290709807E-4
25	2.920425829945197E-4	0.0	0.0	0.05291280617346161	1.0619730290709807E-4
26	2.920425829945197E-4	0.0	0.0	0.06355908578989819	1.0619730290709807E-4
27	2.920425829945197E-4	0.0	0.0	0.11201160524126169	1.0619730290709807E-4
28	2.920425829945197E-4	0.0	0.0	0.22115588330403171	1.0619730290709807E-4
29	2.920425829945197E-4	0.0	0.0	0.3562123032761337	1.0619730290709807E-4
30	2.920425829945197E-4	0.0	0.0	0.5498099864757735	1.0619730290709807E-4
31	2.920425829945197E-4	0.0	0.0	0.8381887625199983	1.0619730290709807E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12070	0.0	21.626762	1
GTATTGG	2200	0.0	17.911364	1
TTAACGG	400	0.0	16.650002	35
ATTGGAC	2485	0.0	16.154928	3
TTGGACC	2980	0.0	15.209731	4
GTCTAGA	260	0.0	14.942307	1
TGGACCC	3030	0.0	14.653466	5
TAATACT	1000	0.0	14.429999	4
TATTGGA	2790	0.0	14.256273	2
GTACTAT	390	0.0	14.23077	1
GTATCAA	18335	0.0	14.1663475	2
TCGGGTA	355	0.0	14.070422	25
GGACCCT	3170	0.0	13.889589	6
TAACGGC	495	0.0	13.828283	36
CTAATAC	900	0.0	13.7722225	3
GTATTAG	820	0.0	13.762195	1
GGACCGT	835	0.0	13.7365265	6
CGAACTA	630	0.0	13.507937	24
ACCGTTA	605	0.0	13.454545	8
AACGGCC	545	0.0	13.238532	37
>>END_MODULE