FastQCFastQC Report
Wed 25 May 2016
SRR2088581_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088581_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4371436
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT485191.10990987858452No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT414180.947468978157292No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT340760.7795150152032421No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175790.40213330356432075No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG107620.24618912412305705No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA105840.24211723561776954No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA96110.21985910350740578No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG91960.2103656555877748No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA81430.18627746122784367No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG72070.16486573290790488No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA60170.1376435569455895No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG50030.11444751793232247No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT48010.10982661075216472No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA47130.10781354227764058No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC45610.10433642400346248No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45530.10415341777850573No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC43730.10003577771697904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150200.020.5815561
GTATTGG22200.018.0833341
TTGGACC29900.016.7056854
ATTGGAC26450.016.2967873
TGGACCC30850.015.5316045
GGACCCT32450.014.7657946
ATTAGAC4350.014.0344833
TATTGGA30400.013.9358562
GTATCAA221650.013.9219492
TAGCGAA5100.013.78431310
TTAACGG5700.013.63157835
GATCGGT1501.3077824E-613.56666711
CGAACTA7100.013.54929524
GTATTAG10950.013.3470331
TTAGACT5550.013.04
TAGATGT9000.012.954
CGGGTAA4150.012.92771126
ACCGTTA6350.012.8188978
GACCGTT6550.012.7099237
ATTAGAG8950.012.6089393
Produced by FastQC (version 0.11.5)