##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088581_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4371436
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.662482076827843	31.0	31.0	34.0	30.0	34.0
2	31.837508315345346	31.0	31.0	34.0	30.0	34.0
3	31.923496306476864	31.0	31.0	34.0	30.0	34.0
4	35.61299673608398	37.0	35.0	37.0	33.0	37.0
5	35.519637254211204	37.0	35.0	37.0	33.0	37.0
6	35.54735972344099	37.0	35.0	37.0	33.0	37.0
7	35.42035980853889	37.0	35.0	37.0	33.0	37.0
8	35.43964111564255	37.0	35.0	37.0	33.0	37.0
9	37.04669929972668	39.0	37.0	39.0	33.0	39.0
10	36.90724077854508	39.0	37.0	39.0	33.0	39.0
11	36.94636956826086	39.0	37.0	39.0	33.0	39.0
12	36.853429856916584	39.0	37.0	39.0	32.0	39.0
13	36.89738657960451	39.0	37.0	39.0	33.0	39.0
14	37.932115899672326	40.0	37.0	41.0	33.0	41.0
15	37.889704207038605	40.0	37.0	41.0	33.0	41.0
16	37.79410404269901	40.0	37.0	41.0	32.0	41.0
17	37.82111599026041	40.0	37.0	41.0	32.0	41.0
18	37.795143289298984	40.0	37.0	41.0	32.0	41.0
19	37.81593073763404	40.0	37.0	41.0	32.0	41.0
20	37.69619891495609	40.0	37.0	41.0	32.0	41.0
21	37.600555515395854	39.0	37.0	41.0	32.0	41.0
22	37.44981740553905	39.0	36.0	41.0	32.0	41.0
23	37.32893378743278	39.0	36.0	41.0	31.0	41.0
24	37.19089791089244	39.0	36.0	41.0	31.0	41.0
25	37.001877643868056	39.0	36.0	41.0	31.0	41.0
26	37.15662564887145	39.0	36.0	41.0	31.0	41.0
27	37.088373019758265	39.0	36.0	41.0	31.0	41.0
28	36.972578576010264	39.0	36.0	41.0	31.0	41.0
29	36.82880362425528	39.0	35.0	41.0	30.0	41.0
30	36.714829634930034	39.0	35.0	40.0	30.0	41.0
31	36.54559371337016	39.0	35.0	40.0	30.0	41.0
32	36.43151335167666	39.0	35.0	40.0	30.0	41.0
33	36.22870882703075	38.0	35.0	40.0	30.0	41.0
34	36.09321765204844	38.0	35.0	40.0	29.0	41.0
35	35.89566197469207	38.0	35.0	40.0	28.0	41.0
36	35.750905194540195	38.0	35.0	40.0	27.0	41.0
37	35.587506256525316	38.0	35.0	40.0	27.0	41.0
38	35.50415835894658	38.0	35.0	40.0	26.0	41.0
39	35.36944747675592	38.0	34.0	40.0	25.0	41.0
40	35.19567780473053	38.0	34.0	40.0	25.0	41.0
41	35.076362778729916	38.0	34.0	40.0	24.0	41.0
42	34.86295578844114	38.0	34.0	40.0	23.0	41.0
43	34.51044142016491	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	2.0
12	2.0
13	8.0
14	15.0
15	31.0
16	66.0
17	177.0
18	332.0
19	761.0
20	1606.0
21	3038.0
22	5685.0
23	9852.0
24	16086.0
25	24760.0
26	36265.0
27	50999.0
28	69511.0
29	91304.0
30	115152.0
31	141812.0
32	173750.0
33	214247.0
34	264685.0
35	327558.0
36	424237.0
37	601218.0
38	830503.0
39	967773.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.058948135120815	20.97086632401801	13.477150300267462	23.49303524059371
2	18.71023617868362	22.978261605568516	35.29435178737605	23.01715042837182
3	19.80651209350886	24.874434853901555	32.18027211195589	23.138780940633698
4	13.579290649571446	16.091256969105807	36.55977120561756	33.76968117570519
5	13.069412431063842	38.25731407253818	34.93350926331759	13.739764233080386
6	32.56675838328641	37.17943028332108	13.93123449594138	16.322576837451123
7	26.438955071056743	32.49332713552251	21.92307058824606	19.144647205174685
8	26.94409800349359	34.56148048375866	19.14887922412681	19.345542288620948
9	24.645311060255715	14.351302409551462	21.765433601223947	39.23795292896888
10	16.358583312211366	27.109444127742005	33.90924629801283	22.622726262033805
11	33.31646625959982	22.206592982260293	23.66528984983424	20.811650908305644
12	21.050565534986674	26.703033053669323	31.257577601502117	20.988823809841893
13	31.292394535800135	20.644772106923217	26.41829824341475	21.6445351138619
14	20.637978000821697	22.419086085213188	27.852998419741247	29.089937494223868
15	23.056519642515642	29.08524795970935	25.377884978757553	22.48034741901746
16	22.14089374750082	27.905910094531865	26.562552900236902	23.39064325773041
17	21.914332041004375	26.351249337746225	27.310545093191347	24.423873528058056
18	23.05208174156044	25.32083278812729	29.384371634401145	22.24271383591113
19	22.979382518696372	24.65910515446183	30.312533455825502	22.0489788710163
20	23.802155630323764	24.34058281992462	30.07654692874378	21.78071462100783
21	22.79532400794613	25.23815972600308	30.42114765033733	21.545368615713464
22	21.662698481688857	25.6526688255301	30.095556700361165	22.589075992419883
23	22.39330508327241	25.712214475975397	30.936081415809358	20.958399024942835
24	22.518183956027265	25.776426785157096	28.77912429691296	22.926264961902678
25	22.522621856982468	25.862782847558556	29.545211230360003	22.069384065098973
26	22.239488351196265	26.465307967450514	29.716253423360193	21.578950257993025
27	21.470679200152993	26.557520229050592	29.386636336434986	22.585164234361432
28	21.793616559867285	26.21342277457568	29.54294652832616	22.450014137230877
29	22.048704361678862	26.235749534020396	29.12111717980087	22.59442892449987
30	21.886057579248558	26.415759032043475	30.37718040479147	21.321002983916497
31	22.524520546566393	26.005916591252852	30.219474790434997	21.25008807174576
32	22.004325352126852	25.885109607003283	29.3462605880539	22.76430445281596
33	21.794028323873437	25.938341542687578	29.989664723445568	22.27796540999342
34	21.367326434608675	26.389703520765256	30.27366750880031	21.969302535825754
35	21.030251844016473	28.14930837372433	29.284404484018523	21.53603529824067
36	22.670994153866143	26.184828051926186	29.403244151349806	21.74093364285786
37	21.82429297832566	26.582660709204024	29.29012342854842	22.302922883921898
38	21.734459797650015	25.351943846369934	30.480075654773398	22.433520701206653
39	21.18715680613876	25.301479879838112	31.05121520708527	22.460148106937858
40	20.38780849130583	26.113295493746218	30.65180412111718	22.84709189383077
41	21.584120183848054	24.965366071926937	30.72534974777167	22.72516399645334
42	20.244651871833423	26.784630954221907	30.237661034040077	22.733056139904598
43	20.64646491450407	26.461602091395136	29.61676666431809	23.275166329782706
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	216.0
1	481.0
2	746.0
3	2406.5
4	4067.0
5	4067.0
6	6136.5
7	8206.0
8	8945.0
9	9684.0
10	13908.0
11	18132.0
12	18132.0
13	32381.0
14	46630.0
15	72648.5
16	98667.0
17	90677.5
18	82688.0
19	82688.0
20	88824.5
21	94961.0
22	76313.5
23	57666.0
24	65865.5
25	74065.0
26	74065.0
27	81762.0
28	89459.0
29	98595.5
30	107732.0
31	118674.0
32	129616.0
33	129616.0
34	143974.0
35	158332.0
36	174481.5
37	190631.0
38	203468.0
39	216305.0
40	216305.0
41	222900.0
42	229495.0
43	245503.0
44	261511.0
45	275773.5
46	290036.0
47	290036.0
48	361780.5
49	433525.0
50	414165.0
51	394805.0
52	369081.5
53	343358.0
54	343358.0
55	308806.5
56	274255.0
57	236218.0
58	198181.0
59	176383.0
60	154585.0
61	154585.0
62	136611.0
63	118637.0
64	103199.5
65	87762.0
66	75592.0
67	63422.0
68	63422.0
69	55418.5
70	47415.0
71	40485.0
72	33555.0
73	28057.0
74	22559.0
75	22559.0
76	17852.5
77	13146.0
78	10403.0
79	7660.0
80	6088.0
81	4516.0
82	4516.0
83	3402.0
84	2288.0
85	1794.0
86	1300.0
87	1028.0
88	756.0
89	756.0
90	653.5
91	551.0
92	359.5
93	168.0
94	134.0
95	100.0
96	100.0
97	68.0
98	36.0
99	23.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4371436.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.70053369202719
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.074547207412	33.24498313343221
2	12.739879026854508	11.134790252908122
3	4.483617205747358	5.878093942857458
4	2.0559124799554596	3.5937789039261108
5	1.1471310354818982	2.5065119232623365
6	0.6920497845159208	1.81457669548789
7	0.45005922559997813	1.376747984621766
8	0.33500139871492435	1.171179192913423
9	0.2391700824585197	0.9406674221943115
>10	1.5061715759022873	12.536064461562951
>50	0.153718260711986	4.687294299108123
>100	0.10818685208993667	9.430561300821044
>500	0.0086916314020219	2.660907583346509
>1k	0.005235922458234269	4.261909358057122
>5k	3.141553474940558E-4	1.0337067091996572
>10k+	3.141553474940558E-4	3.7282268363010767
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	48519	1.10990987858452	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	41418	0.947468978157292	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	34076	0.7795150152032421	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17579	0.40213330356432075	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10762	0.24618912412305705	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10584	0.24211723561776954	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9611	0.21985910350740578	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9196	0.2103656555877748	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	8143	0.18627746122784367	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7207	0.16486573290790488	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6017	0.1376435569455895	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	5003	0.11444751793232247	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4801	0.10982661075216472	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4713	0.10781354227764058	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4561	0.10433642400346248	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4553	0.10415341777850573	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4373	0.10003577771697904	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.287577811959274E-5	0.0
3	2.287577811959274E-5	0.0	0.0	2.287577811959274E-5	0.0
4	4.575155623918548E-5	0.0	0.0	2.287577811959274E-5	0.0
5	6.862733435877822E-5	0.0	0.0	2.287577811959274E-5	0.0
6	9.150311247837096E-5	0.0	0.0	2.287577811959274E-5	0.0
7	9.150311247837096E-5	0.0	0.0	2.287577811959274E-5	0.0
8	1.1437889059796368E-4	0.0	0.0	2.287577811959274E-5	0.0
9	2.5163355931552014E-4	0.0	0.0	1.1437889059796368E-4	0.0
10	2.973851155547056E-4	0.0	0.0	1.1437889059796368E-4	0.0
11	3.6601244991348383E-4	2.287577811959274E-5	0.0	2.973851155547056E-4	0.0
12	3.6601244991348383E-4	2.287577811959274E-5	0.0	5.718944529898185E-4	0.0
13	3.6601244991348383E-4	2.287577811959274E-5	0.0	6.405217873485967E-4	0.0
14	4.803913405114475E-4	2.287577811959274E-5	0.0	8.464037904249313E-4	0.0
15	4.803913405114475E-4	2.287577811959274E-5	0.0	0.0010294100153816733	0.0
16	5.032671186310403E-4	2.287577811959274E-5	0.0	0.0015784286902518988	2.287577811959274E-5
17	5.032671186310403E-4	2.287577811959274E-5	0.0	0.0024019567025572374	2.287577811959274E-5
18	5.26142896750633E-4	2.287577811959274E-5	0.0	0.002950975377427463	2.287577811959274E-5
19	5.718944529898185E-4	2.287577811959274E-5	0.0	0.003957509614689544	2.287577811959274E-5
20	5.947702311094112E-4	2.287577811959274E-5	0.0	0.006450969429725152	2.287577811959274E-5
21	5.947702311094112E-4	2.287577811959274E-5	0.0	0.011872528844068631	2.287577811959274E-5
22	5.947702311094112E-4	2.287577811959274E-5	0.0	0.020245063635839573	2.287577811959274E-5
23	6.405217873485967E-4	2.287577811959274E-5	0.0	0.02674178462180391	2.287577811959274E-5
24	7.091491217073749E-4	2.287577811959274E-5	0.0	0.03632673565391327	2.287577811959274E-5
25	7.091491217073749E-4	2.287577811959274E-5	0.0	0.044150251770813984	2.287577811959274E-5
26	7.091491217073749E-4	2.287577811959274E-5	0.0	0.05307180523745515	2.287577811959274E-5
27	7.091491217073749E-4	2.287577811959274E-5	0.0	0.09653578366468135	2.287577811959274E-5
28	7.091491217073749E-4	2.287577811959274E-5	0.0	0.19899639386233722	2.287577811959274E-5
29	7.320248998269677E-4	2.287577811959274E-5	0.0	0.3336203480961405	2.287577811959274E-5
30	8.235280123053385E-4	2.287577811959274E-5	0.0	0.5146821319127171	6.862733435877822E-5
31	8.69279568544524E-4	2.287577811959274E-5	0.0	0.7914332956035499	6.862733435877822E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15020	0.0	20.581556	1
GTATTGG	2220	0.0	18.083334	1
TTGGACC	2990	0.0	16.705685	4
ATTGGAC	2645	0.0	16.296787	3
TGGACCC	3085	0.0	15.531604	5
GGACCCT	3245	0.0	14.765794	6
ATTAGAC	435	0.0	14.034483	3
TATTGGA	3040	0.0	13.935856	2
GTATCAA	22165	0.0	13.921949	2
TAGCGAA	510	0.0	13.784313	10
TTAACGG	570	0.0	13.631578	35
GATCGGT	150	1.3077824E-6	13.566667	11
CGAACTA	710	0.0	13.549295	24
GTATTAG	1095	0.0	13.347033	1
TTAGACT	555	0.0	13.0	4
TAGATGT	900	0.0	12.95	4
CGGGTAA	415	0.0	12.927711	26
ACCGTTA	635	0.0	12.818897	8
GACCGTT	655	0.0	12.709923	7
ATTAGAG	895	0.0	12.608939	3
>>END_MODULE