FastQCFastQC Report
Wed 25 May 2016
SRR2088580_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088580_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4362939
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT544331.247622302305854No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT475381.0895866295632373No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT389620.8930218827262998No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214230.49102222148877167No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG133360.30566551583691637No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA128010.2934031394892296No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA117130.26846582086066295No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG103020.23612523576424055No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA96040.2201268456881932No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG83880.19225572486803047No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA75280.1725442413932443No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG64150.14703391452413156No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC60620.1389430381676205No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA58610.13433605191362977No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT57330.13140224972203368No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56080.12853720851930317No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC53720.12312801072854788No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA50470.11567890360144846No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC49980.11455580744997809No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49850.11425784316489412No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA46450.10646493109346704No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC44860.10282059868359379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA185850.018.9230581
GTATTGG25750.017.099031
ATTAGAG7300.016.219183
TAATACT10000.016.0954
GTATTAG9450.016.052911
TGGACCC34800.015.4698295
CTAATAC9250.015.43
TTAACGG5700.015.25438735
TACGTTA1353.9792576E-715.07407519
ATTGGAC30700.014.8241043
TTGGACC37100.014.8099734
CCGTTTA3650.014.69863127
GGACCCT37300.014.3833786
TCTAACG2450.014.3469382
ATACTGG9050.014.3093926
TAACGGC6150.014.13821136
TATTGGA33000.013.903032
TATTAGA9350.013.8502662
CGTTCGC7000.013.74285823
TCGGGTA3100.013.72580625
Produced by FastQC (version 0.11.5)