FastQCFastQC Report
Wed 25 May 2016
SRR2088579_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088579_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8894656
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1020001.1467559847171156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT932661.0485621928492794No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT713040.8016498895516588No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT387660.435834730426899No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG234630.26378760460213413No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA225550.2535792277970053No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA217270.2442702674504781No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG207240.23299383360075981No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA194010.2181197339166349No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG163100.18336853049741328No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA128240.14417645831384598No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA108510.12199459990358255No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT107960.12137625108829392No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102350.11506909317234978No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG99520.11188740745004641No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA99300.11164006792393096No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC97850.11000987559271545No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97730.10987496312392521No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC94630.10638972434684378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA308850.022.396471
GTATTGG44450.017.9797521
TATACCG5350.016.2523375
GTATTAG20350.015.5454551
ATTGGAC52500.015.4342853
ACCGTTA13850.015.3610118
GTATCAA462900.014.9230942
TTAACGG12800.014.88671935
TTGGACC64100.014.48834
TATTGGA57400.014.3423352
TGGACCC63750.014.161575
TAATACT22800.013.9561414
TGTTACG8550.013.63157916
GACCGTT15900.013.6132077
GGACCCT67550.013.4470776
ATTAGAG17750.013.4450713
AGTTCCG8950.013.43575532
TATACTG9350.013.2566845
CTAATAC22100.013.2262443
TAGCGAA11800.013.01271210
Produced by FastQC (version 0.11.5)