##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088579_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8894656
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.595393346296923	31.0	31.0	34.0	30.0	34.0
2	31.779433516034796	31.0	31.0	34.0	30.0	34.0
3	31.852581595061125	31.0	31.0	34.0	30.0	34.0
4	35.559097282683	37.0	35.0	37.0	33.0	37.0
5	35.46308154019672	37.0	35.0	37.0	33.0	37.0
6	35.495145961799984	37.0	35.0	37.0	33.0	37.0
7	35.359952875074654	37.0	35.0	37.0	33.0	37.0
8	35.38385138222321	37.0	35.0	37.0	33.0	37.0
9	36.970283954770146	39.0	37.0	39.0	33.0	39.0
10	36.82429371074047	39.0	37.0	39.0	32.0	39.0
11	36.870799612711274	39.0	37.0	39.0	32.0	39.0
12	36.773072055850164	39.0	37.0	39.0	32.0	39.0
13	36.818531374344325	39.0	37.0	39.0	32.0	39.0
14	37.83093241604847	40.0	37.0	41.0	32.0	41.0
15	37.7875902114708	40.0	37.0	41.0	32.0	41.0
16	37.6801252347477	39.0	37.0	41.0	32.0	41.0
17	37.719917442563265	39.0	37.0	41.0	32.0	41.0
18	37.694340961584125	39.0	37.0	41.0	32.0	41.0
19	37.72318906993143	40.0	37.0	41.0	32.0	41.0
20	37.600860224386416	39.0	37.0	41.0	32.0	41.0
21	37.50167044121774	39.0	36.0	41.0	32.0	41.0
22	37.35222553857057	39.0	36.0	41.0	31.0	41.0
23	37.219102346397655	39.0	36.0	41.0	31.0	41.0
24	37.07465291518862	39.0	36.0	41.0	31.0	41.0
25	36.88145893444333	39.0	36.0	40.0	30.0	41.0
26	37.0202746458098	39.0	36.0	41.0	31.0	41.0
27	36.94648562012966	39.0	36.0	41.0	31.0	41.0
28	36.82340261388411	39.0	35.0	41.0	30.0	41.0
29	36.67224297375863	39.0	35.0	40.0	30.0	41.0
30	36.55482303081761	39.0	35.0	40.0	30.0	41.0
31	36.36530822552328	38.0	35.0	40.0	30.0	41.0
32	36.24566447538837	38.0	35.0	40.0	30.0	41.0
33	36.04047632645939	38.0	35.0	40.0	29.0	41.0
34	35.89807857661949	38.0	35.0	40.0	29.0	41.0
35	35.706765275689136	38.0	35.0	40.0	27.0	41.0
36	35.568782311536275	38.0	35.0	40.0	27.0	41.0
37	35.402075246080344	38.0	34.0	40.0	26.0	41.0
38	35.31275217388958	38.0	34.0	40.0	25.0	41.0
39	35.16855356744544	38.0	34.0	40.0	25.0	41.0
40	34.97955053011606	38.0	34.0	40.0	24.0	41.0
41	34.84768629613107	38.0	34.0	40.0	24.0	41.0
42	34.62594247602156	38.0	33.0	40.0	23.0	41.0
43	34.269098209081946	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	5.0
12	6.0
13	6.0
14	38.0
15	56.0
16	134.0
17	302.0
18	737.0
19	1647.0
20	3569.0
21	6972.0
22	12996.0
23	22187.0
24	35556.0
25	53978.0
26	79183.0
27	110284.0
28	149108.0
29	195089.0
30	245595.0
31	300719.0
32	366604.0
33	450609.0
34	554834.0
35	682206.0
36	885518.0
37	1242303.0
38	1693783.0
39	1800632.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.67427048331043	21.185417401190108	13.810314867713828	23.329997247785634
2	19.226342199181172	22.99525692730556	34.789001395894346	22.98939947761892
3	20.151695579907756	24.493190068283695	32.211746019182755	23.14336833262579
4	14.105593291072752	16.169158200159735	35.85714838212967	33.86810012663784
5	13.119765396210939	38.159530846386865	34.71965638693615	14.001047370466042
6	32.833737471128735	36.712234852028004	13.567641064477368	16.886386612365897
7	26.403584354470823	32.53065660999144	21.511152314378432	19.554606721159313
8	26.978525082926197	34.47703879722836	18.902372390792856	19.64206372905259
9	24.545187582296606	14.34267946956015	21.690956907158636	39.421176040984605
10	16.798018945308286	26.978839878686706	33.394332507069414	22.828808668935594
11	33.41630075407076	22.09810025255614	23.37921781348261	21.106381179890487
12	21.09292366112866	26.76462136365926	30.85855147180509	21.28390350340699
13	31.574981651904245	20.522187704617245	26.31457585318645	21.58825479029206
14	20.671603263802446	22.497755955935787	27.527371491376396	29.30326928888537
15	23.15545424128825	28.95108028910843	25.350581292857193	22.542884176746128
16	22.18755846206981	27.802851509940353	26.56009406097324	23.449495967016603
17	22.041380802135574	26.30342308909979	26.97217295418732	24.68302315457731
18	23.12064682434037	25.16073696385785	29.3236410716727	22.394975140129084
19	23.102175058821835	24.407453194367495	30.32913245886069	22.16123928794998
20	23.958981662697244	24.108273552119385	30.179379618503514	21.75336516667986
21	22.938031555127033	24.94332552040236	30.57005240000288	21.548590524467727
22	21.722627609207144	25.320664452902957	30.14457220155563	22.812135736334266
23	22.531562772073478	25.556738787874426	31.11842661481231	20.793271825239785
24	22.638525874412682	25.5436972492247	28.752174339288672	23.065602537073946
25	22.604910184272445	25.646691676440327	29.68184491901654	22.066553220270688
26	22.340796541204067	26.329123914404335	29.735427654537737	21.594651889853864
27	21.357531983249267	26.431140226221228	29.372366958317443	22.83896083221206
28	21.751566333762653	26.070923934551267	29.603247163240486	22.574262568445594
29	22.091152260413445	25.97711479791911	29.06302391008714	22.868709031580313
30	22.010969283129107	26.060310820339765	30.579541243641124	21.349178652890004
31	22.57330693845833	25.690482015268497	30.485923233006424	21.250287813266752
32	22.17810334654876	25.572962012246453	29.477396315270653	22.771538325934134
33	21.821237381187085	25.713068611085127	30.02170067060491	22.443993337122876
34	21.327896210938345	26.193075932335102	30.427899628720883	22.05112822800567
35	20.92593575288353	27.990559724850517	29.396561261053826	21.686943261212125
36	22.82745954424769	25.71786924643291	29.45401148734701	22.000659721972387
37	21.835200821706877	26.30180413947431	29.41748393642205	22.445511102396765
38	21.747406532641623	25.040698594751724	30.652067938321615	22.559826934285034
39	21.228049741327826	24.921312302578087	31.330655170925105	22.51998278516898
40	20.29609689233625	25.668671166147405	30.904264313313522	23.13096762820282
41	21.487924884335044	24.567515595881392	31.005212568085827	22.93934695169774
42	20.23675789148001	26.49042301354881	30.417455155095375	22.855363939875808
43	20.54897907237784	26.33061919426676	29.648296685110697	23.4721050482447
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	628.0
1	1144.5
2	1661.0
3	4375.5
4	7090.0
5	7090.0
6	11389.0
7	15688.0
8	16906.5
9	18125.0
10	27379.0
11	36633.0
12	36633.0
13	68524.5
14	100416.0
15	154161.5
16	207907.0
17	189819.5
18	171732.0
19	171732.0
20	185687.5
21	199643.0
22	157513.0
23	115383.0
24	128578.5
25	141774.0
26	141774.0
27	154967.5
28	168161.0
29	185260.5
30	202360.0
31	223045.0
32	243730.0
33	243730.0
34	273061.0
35	302392.0
36	334709.5
37	367027.0
38	394215.0
39	421403.0
40	421403.0
41	439434.5
42	457466.0
43	492728.0
44	527990.0
45	561006.5
46	594023.0
47	594023.0
48	757406.5
49	920790.0
50	874750.5
51	828711.0
52	768548.5
53	708386.0
54	708386.0
55	632934.5
56	557483.0
57	481816.5
58	406150.0
59	365372.5
60	324595.0
61	324595.0
62	289840.0
63	255085.0
64	222481.5
65	189878.0
66	162593.5
67	135309.0
68	135309.0
69	116895.0
70	98481.0
71	83381.0
72	68281.0
73	55984.5
74	43688.0
75	43688.0
76	34712.5
77	25737.0
78	20111.0
79	14485.0
80	11257.0
81	8029.0
82	8029.0
83	6046.0
84	4063.0
85	3083.0
86	2103.0
87	1583.5
88	1064.0
89	1064.0
90	860.0
91	656.0
92	462.5
93	269.0
94	196.5
95	124.0
96	124.0
97	81.0
98	38.0
99	28.5
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8894656.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.326769276166836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.10558183088158	26.19362834694202
2	13.34889960994933	9.698447924426857
3	5.413629807470396	5.899790428876697
4	2.7095500330586786	3.937167955726114
5	1.589048801502203	2.8862504590369977
6	0.9942075910679394	2.16698098640032
7	0.6861759195504146	1.7448588018658668
8	0.4809761613855411	1.397784803359115
9	0.3472460753705769	1.1352895255837248
>10	1.9616691979628855	13.365801594856059
>50	0.194937493838206	4.947597574292
>100	0.1437179108191228	10.206720397673408
>500	0.015383837288071106	3.877856565655641
>1k	0.00795435371225861	5.6202835663036375
>5k	5.88065051956093E-4	1.5896625865516225
>10k+	4.3331109091501583E-4	5.331878482450056
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	102000	1.1467559847171156	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	93266	1.0485621928492794	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	71304	0.8016498895516588	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38766	0.435834730426899	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	23463	0.26378760460213413	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	22555	0.2535792277970053	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	21727	0.2442702674504781	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	20724	0.23299383360075981	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	19401	0.2181197339166349	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	16310	0.18336853049741328	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	12824	0.14417645831384598	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	10851	0.12199459990358255	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10796	0.12137625108829392	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10235	0.11506909317234978	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	9952	0.11188740745004641	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	9930	0.11164006792393096	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	9785	0.11000987559271545	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9773	0.10987496312392521	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	9463	0.10638972434684378	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.248541146504148E-5	0.0	0.0	2.248541146504148E-5	0.0
6	6.745623439512444E-5	0.0	0.0	2.248541146504148E-5	0.0
7	6.745623439512444E-5	0.0	1.124270573252074E-5	4.497082293008296E-5	0.0
8	1.349124687902489E-4	0.0	1.124270573252074E-5	5.62135286626037E-5	0.0
9	1.4615517452276963E-4	0.0	1.124270573252074E-5	2.810676433130185E-4	0.0
10	2.0236870318537332E-4	0.0	1.124270573252074E-5	4.0473740637074663E-4	0.0
11	2.1361140891789406E-4	0.0	1.124270573252074E-5	7.082904611488066E-4	0.0
12	2.1361140891789406E-4	0.0	1.124270573252074E-5	0.0011804841019146777	0.0
13	2.1361140891789406E-4	0.0	1.124270573252074E-5	0.0012479403363098022	0.0
14	2.698249375804978E-4	0.0	2.248541146504148E-5	0.0014952798624252586	0.0
15	2.810676433130185E-4	0.0	2.248541146504148E-5	0.0018550464458659223	0.0
16	3.0355305477806E-4	0.0	2.248541146504148E-5	0.002608307729944812	0.0
17	3.147957605105807E-4	0.0	2.248541146504148E-5	0.003721335597464365	0.0
18	3.260384662431015E-4	0.0	2.248541146504148E-5	0.004463354175810734	0.0
19	3.260384662431015E-4	0.0	2.248541146504148E-5	0.005452712280272559	0.0
20	3.372811719756222E-4	0.0	2.248541146504148E-5	0.008600669885378366	0.0
21	3.372811719756222E-4	0.0	2.248541146504148E-5	0.015278837090495686	0.0
22	3.372811719756222E-4	0.0	2.248541146504148E-5	0.025161175429381417	0.0
23	3.48523877708143E-4	0.0	3.372811719756222E-5	0.03216538110074184	0.0
24	3.934947006382259E-4	0.0	3.372811719756222E-5	0.04303707754408939	0.0
25	3.934947006382259E-4	0.0	3.372811719756222E-5	0.05022116650717015	0.0
26	3.934947006382259E-4	0.0	3.372811719756222E-5	0.06063191201548435	0.0
27	4.0473740637074663E-4	0.0	3.372811719756222E-5	0.11232587297361472	0.0
28	4.0473740637074663E-4	0.0	3.372811719756222E-5	0.2290701293001101	0.0
29	4.0473740637074663E-4	0.0	3.372811719756222E-5	0.3805768317515596	0.0
30	4.2722281783578813E-4	0.0	3.372811719756222E-5	0.5893201490872721	0.0
31	4.497082293008296E-4	0.0	3.372811719756222E-5	0.888724645450032	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	30885	0.0	22.39647	1
GTATTGG	4445	0.0	17.979752	1
TATACCG	535	0.0	16.252337	5
GTATTAG	2035	0.0	15.545455	1
ATTGGAC	5250	0.0	15.434285	3
ACCGTTA	1385	0.0	15.361011	8
GTATCAA	46290	0.0	14.923094	2
TTAACGG	1280	0.0	14.886719	35
TTGGACC	6410	0.0	14.4883	4
TATTGGA	5740	0.0	14.342335	2
TGGACCC	6375	0.0	14.16157	5
TAATACT	2280	0.0	13.956141	4
TGTTACG	855	0.0	13.631579	16
GACCGTT	1590	0.0	13.613207	7
GGACCCT	6755	0.0	13.447077	6
ATTAGAG	1775	0.0	13.445071	3
AGTTCCG	895	0.0	13.435755	32
TATACTG	935	0.0	13.256684	5
CTAATAC	2210	0.0	13.226244	3
TAGCGAA	1180	0.0	13.012712	10
>>END_MODULE