FastQCFastQC Report
Wed 25 May 2016
SRR2088578_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088578_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597579
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT68691.1494714506366523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT55680.9317596501885106No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47880.8012329750543442No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24440.4089835820870546No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG14760.2469966314077302No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA13630.22808699770239582No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA13450.22507484366083813No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG12120.2028183721315508No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA10870.18190063573184465No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG9430.15780340339938317No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA7230.12098818733590035No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA6990.11697198194715677No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC6420.10743349414889078No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6330.10592741712811193No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6280.10509070767212368No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA6070.10157652795697306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTA604.3155524E-827.7500028
TACTACG358.864392E-426.4285722
AGGGCGT551.9006777E-523.5454546
GGTATCA19150.021.6396881
CTATATC603.722433E-521.5833344
TGTTACG450.003823690820.55555516
GTCTTAT450.003823690820.5555551
ACAGTCC500.007031025418.58
GTATTGG3000.018.51
CGTTATT500.007031025418.510
GTGTAGT609.2297414E-418.51
GACATTC500.007031025418.57
GTATAGC701.2179545E-418.59
CGTTTAC1002.869183E-718.528
CCGTTTA902.1486994E-618.527
CCGTTAT953.6005276E-617.5263169
ATTGGAC3700.017.53
GTATTAA752.0651746E-417.2666661
TACCCTA1401.8626451E-917.1785725
TTGGACC4100.017.1463414
Produced by FastQC (version 0.11.5)