##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088578_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	597579
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.716092767650803	31.0	31.0	34.0	30.0	34.0
2	31.896375207294767	31.0	31.0	34.0	30.0	34.0
3	31.98587634438292	31.0	31.0	34.0	30.0	34.0
4	35.65814896440471	37.0	35.0	37.0	33.0	37.0
5	35.56711330217427	37.0	35.0	37.0	33.0	37.0
6	35.60281067440456	37.0	35.0	37.0	33.0	37.0
7	35.46079263160185	37.0	35.0	37.0	33.0	37.0
8	35.48406821524853	37.0	35.0	37.0	33.0	37.0
9	37.09631362547881	39.0	37.0	39.0	33.0	39.0
10	36.949072842251816	39.0	37.0	39.0	33.0	39.0
11	37.00358111647163	39.0	37.0	39.0	33.0	39.0
12	36.902248907675805	39.0	37.0	39.0	33.0	39.0
13	36.94687229638257	39.0	37.0	39.0	33.0	39.0
14	37.97821041234715	40.0	37.0	41.0	33.0	41.0
15	37.935467946497454	40.0	37.0	41.0	33.0	41.0
16	37.85172504388541	40.0	37.0	41.0	32.0	41.0
17	37.86702176615979	40.0	37.0	41.0	32.0	41.0
18	37.84814225399487	40.0	37.0	41.0	32.0	41.0
19	37.865000276114124	40.0	37.0	41.0	32.0	41.0
20	37.76548874709453	40.0	37.0	41.0	32.0	41.0
21	37.659079385319764	40.0	37.0	41.0	32.0	41.0
22	37.504919014891755	39.0	36.0	41.0	32.0	41.0
23	37.37065726874606	39.0	36.0	41.0	31.0	41.0
24	37.23472712394512	39.0	36.0	41.0	31.0	41.0
25	37.04784472011232	39.0	36.0	41.0	31.0	41.0
26	37.1936313023048	39.0	36.0	41.0	31.0	41.0
27	37.125861183207576	39.0	36.0	41.0	31.0	41.0
28	36.99515712566874	39.0	36.0	41.0	31.0	41.0
29	36.86241986415185	39.0	35.0	41.0	30.0	41.0
30	36.72784853550744	39.0	35.0	41.0	30.0	41.0
31	36.556209304543835	39.0	35.0	40.0	30.0	41.0
32	36.44409191086032	39.0	35.0	40.0	30.0	41.0
33	36.246790800881556	38.0	35.0	40.0	30.0	41.0
34	36.0955589135495	38.0	35.0	40.0	29.0	41.0
35	35.91568144128224	38.0	35.0	40.0	29.0	41.0
36	35.76870505824335	38.0	35.0	40.0	27.0	41.0
37	35.607753953870535	38.0	35.0	40.0	27.0	41.0
38	35.521956092834586	38.0	35.0	40.0	26.0	41.0
39	35.37508513518715	38.0	34.0	40.0	25.0	41.0
40	35.204711008920995	38.0	34.0	40.0	25.0	41.0
41	35.07577742859103	38.0	34.0	40.0	24.0	41.0
42	34.85365449589092	38.0	34.0	40.0	23.0	41.0
43	34.49656196084534	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	4.0
14	1.0
15	4.0
16	4.0
17	15.0
18	46.0
19	90.0
20	213.0
21	411.0
22	748.0
23	1311.0
24	2136.0
25	3239.0
26	4883.0
27	6875.0
28	9103.0
29	12205.0
30	15420.0
31	19081.0
32	23648.0
33	28935.0
34	36222.0
35	44532.0
36	57945.0
37	83485.0
38	113093.0
39	133930.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.923159950399864	21.05127522888187	13.568582563978989	23.456982256739277
2	18.76270752486282	22.990935089753822	35.11753257728267	23.12882480810069
3	19.75788975181524	24.399117104182043	32.254814844564486	23.588178299438233
4	13.718855582274477	15.937307033881712	36.53291029303239	33.810927090811425
5	13.040451555359208	38.244315814310745	34.89898406737854	13.816248562951507
6	32.53929605960049	37.20846950779729	13.877328353238653	16.374906079363566
7	26.343964563681123	32.535279854211744	21.844141109376334	19.276614472730802
8	27.008981239300578	34.28383527533598	19.237791153972946	19.469392331390495
9	24.721082902846316	14.342706152659312	21.80883197033363	39.127378974160735
10	16.489869958616353	27.125283853682948	33.62350417267005	22.76134201503065
11	33.481932932716845	22.23672518612602	23.388874107021834	20.892467774135305
12	20.90552044164872	26.811183123904957	31.022676499676194	21.26061993477013
13	31.092792752088013	20.729142088326398	26.340115700183574	21.83794945940202
14	20.62488809011026	22.379467819317615	27.570413284268692	29.425230806303432
15	22.974368242525255	29.351265690394072	25.216582242682556	22.457783824398113
16	22.19271426874104	28.02792601480306	26.336936204250822	23.442423512205078
17	21.854683648521785	26.550297115527822	27.165947933243974	24.429071302706422
18	22.858902337598877	25.426261632353214	29.51107719648783	22.20375883356008
19	22.93905910348255	24.828851080777603	30.191321984206272	22.04076783153357
20	23.82262428900614	24.589719518256164	29.809782472275632	21.777873720462065
21	22.768203032569755	25.34493347323116	30.353476276776792	21.533387217422298
22	21.7111043058742	25.841102180632188	29.944827378472134	22.50296613502148
23	22.337967030300597	25.892308799338664	30.82002546943584	20.9496987009249
24	22.47016712434674	26.01011749074181	28.635711763632926	22.884003621278527
25	22.508488417431	25.999742293487554	29.481959707419435	22.009809581662008
26	22.209281115969603	26.63329869356186	29.67323148905835	21.48418870141019
27	21.381273438323635	26.80950970499298	29.164010114143906	22.645206742539482
28	21.697382270795995	26.43299044979827	29.443638414335176	22.425988865070558
29	22.027715164020155	26.417427653916885	28.98796644460398	22.56689073745898
30	21.841630981008368	26.440353493010964	30.410037836001603	21.307977689979065
31	22.359888818047487	26.14148087533196	30.280180528432226	21.218449778188322
32	21.966970057515407	26.01647648260732	29.22458787875745	22.79196558111982
33	21.71562253693654	26.130603652404115	29.893955443548048	22.259818367111293
34	21.338266572285843	26.54895838039824	30.202031865242922	21.910743182073
35	20.90769588623429	28.23308717341138	29.295038814951663	21.564178125402666
36	22.700764250417098	26.292423261192244	29.16718961007666	21.839622878313996
37	21.795611960929016	26.64066173677455	29.2356324435765	22.328093858719935
38	21.668934149292397	25.488680157769934	30.443338872349933	22.39904682058774
39	21.064662580177686	25.51394878334078	31.004770917318048	22.416617719163494
40	20.3941236221487	26.13830137939921	30.642977748548727	22.824597249903363
41	21.445030698869942	24.97460586801076	30.696527153731974	22.883836279387328
42	20.29388582932131	26.764662078152007	30.251230381255027	22.690221711271647
43	20.66555216967129	26.503776069774876	29.471584510165183	23.35908725038865
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	44.0
1	80.5
2	117.0
3	333.0
4	549.0
5	549.0
6	814.0
7	1079.0
8	1178.5
9	1278.0
10	1888.0
11	2498.0
12	2498.0
13	4428.5
14	6359.0
15	9945.0
16	13531.0
17	12462.5
18	11394.0
19	11394.0
20	11969.0
21	12544.0
22	10099.0
23	7654.0
24	8718.0
25	9782.0
26	9782.0
27	10800.5
28	11819.0
29	12961.0
30	14103.0
31	15700.5
32	17298.0
33	17298.0
34	19494.0
35	21690.0
36	23982.0
37	26274.0
38	28186.5
39	30099.0
40	30099.0
41	31176.5
42	32254.0
43	34610.5
44	36967.0
45	38640.5
46	40314.0
47	40314.0
48	49999.0
49	59684.0
50	56621.5
51	53559.0
52	50022.5
53	46486.0
54	46486.0
55	41682.0
56	36878.0
57	32122.5
58	27367.0
59	24443.0
60	21519.0
61	21519.0
62	19056.0
63	16593.0
64	14382.0
65	12171.0
66	10416.0
67	8661.0
68	8661.0
69	7375.0
70	6089.0
71	5225.5
72	4362.0
73	3578.0
74	2794.0
75	2794.0
76	2216.5
77	1639.0
78	1300.5
79	962.0
80	746.0
81	530.0
82	530.0
83	409.5
84	289.0
85	223.5
86	158.0
87	130.5
88	103.0
89	103.0
90	77.5
91	52.0
92	37.5
93	23.0
94	14.5
95	6.0
96	6.0
97	5.5
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	597579.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.3882941028295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.83248101577553	51.83277107156182
2	7.85670959327886	9.489065791988915
3	2.3326177206521193	4.22588414832636
4	1.069017278072656	2.5822451915703124
5	0.6524601623014946	1.9700478085721265
6	0.4271845890926485	1.5478169161393935
7	0.3038220468036584	1.2843106582112065
8	0.21965971121224662	1.0611900194582193
9	0.16612493957073649	0.9028801537751171
>10	1.0103773528368878	11.371337468554826
>50	0.08034051240548401	3.2962519632917986
>100	0.04336718738779353	4.7544250306751294
>500	0.0033359374913684427	1.2914724678147635
>1k	0.0019459635366315914	2.30242361836468
>5k	5.559895818947404E-4	2.087877691695335
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6869	1.1494714506366523	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5568	0.9317596501885106	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4788	0.8012329750543442	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2444	0.4089835820870546	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1476	0.2469966314077302	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1363	0.22808699770239582	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1345	0.22507484366083813	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1212	0.2028183721315508	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1087	0.18190063573184465	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	943	0.15780340339938317	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	723	0.12098818733590035	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	699	0.11697198194715677	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	642	0.10743349414889078	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	633	0.10592741712811193	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	628	0.10509070767212368	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	607	0.10157652795697306	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.6734189119764918E-4	0.0
4	0.0	0.0	0.0	1.6734189119764918E-4	0.0
5	0.0	0.0	0.0	1.6734189119764918E-4	0.0
6	0.0	0.0	0.0	1.6734189119764918E-4	0.0
7	0.0	0.0	0.0	1.6734189119764918E-4	0.0
8	0.0	0.0	0.0	1.6734189119764918E-4	0.0
9	0.0	0.0	0.0	1.6734189119764918E-4	0.0
10	1.6734189119764918E-4	0.0	0.0	1.6734189119764918E-4	0.0
11	1.6734189119764918E-4	0.0	0.0	3.3468378239529835E-4	0.0
12	1.6734189119764918E-4	0.0	0.0	3.3468378239529835E-4	1.6734189119764918E-4
13	1.6734189119764918E-4	0.0	0.0	3.3468378239529835E-4	1.6734189119764918E-4
14	3.3468378239529835E-4	0.0	0.0	5.020256735929475E-4	3.3468378239529835E-4
15	3.3468378239529835E-4	0.0	0.0	8.367094559882459E-4	3.3468378239529835E-4
16	3.3468378239529835E-4	0.0	0.0	0.0013387351295811934	3.3468378239529835E-4
17	3.3468378239529835E-4	0.0	0.0	0.0016734189119764918	3.3468378239529835E-4
18	3.3468378239529835E-4	0.0	0.0	0.002175444585569439	3.3468378239529835E-4
19	3.3468378239529835E-4	0.0	0.0	0.003514179715150633	3.3468378239529835E-4
20	3.3468378239529835E-4	0.0	0.0	0.006526333756708318	3.3468378239529835E-4
21	3.3468378239529835E-4	0.0	0.0	0.01255064183982369	3.3468378239529835E-4
22	3.3468378239529835E-4	0.0	0.0	0.02208912963808969	3.3468378239529835E-4
23	3.3468378239529835E-4	0.0	0.0	0.02710938637401917	3.3468378239529835E-4
24	3.3468378239529835E-4	0.0	0.0	0.03514179715150633	3.3468378239529835E-4
25	3.3468378239529835E-4	0.0	0.0	0.041500789017016995	3.3468378239529835E-4
26	3.3468378239529835E-4	0.0	0.0	0.05154130248887595	3.3468378239529835E-4
27	3.3468378239529835E-4	0.0	0.0	0.09638892932984593	3.3468378239529835E-4
28	3.3468378239529835E-4	0.0	0.0	0.19779811539562134	3.3468378239529835E-4
29	3.3468378239529835E-4	0.0	0.0	0.3388673296752396	3.3468378239529835E-4
30	3.3468378239529835E-4	0.0	0.0	0.5219393586454678	3.3468378239529835E-4
31	3.3468378239529835E-4	0.0	0.0	0.7742909305715228	3.3468378239529835E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTTA	60	4.3155524E-8	27.750002	8
TACTACG	35	8.864392E-4	26.428572	2
AGGGCGT	55	1.9006777E-5	23.545454	6
GGTATCA	1915	0.0	21.639688	1
CTATATC	60	3.722433E-5	21.583334	4
TGTTACG	45	0.0038236908	20.555555	16
GTCTTAT	45	0.0038236908	20.555555	1
ACAGTCC	50	0.0070310254	18.5	8
GTATTGG	300	0.0	18.5	1
CGTTATT	50	0.0070310254	18.5	10
GTGTAGT	60	9.2297414E-4	18.5	1
GACATTC	50	0.0070310254	18.5	7
GTATAGC	70	1.2179545E-4	18.5	9
CGTTTAC	100	2.869183E-7	18.5	28
CCGTTTA	90	2.1486994E-6	18.5	27
CCGTTAT	95	3.6005276E-6	17.526316	9
ATTGGAC	370	0.0	17.5	3
GTATTAA	75	2.0651746E-4	17.266666	1
TACCCTA	140	1.8626451E-9	17.178572	5
TTGGACC	410	0.0	17.146341	4
>>END_MODULE