FastQCFastQC Report
Wed 25 May 2016
SRR2088577_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088577_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1596937
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT195301.2229662159496586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT155090.9711716867979137No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT121500.7608315168350411No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67420.4221832169960368No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG38620.24183796856106407No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA37530.2350124018668238No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA35860.22455488225271253No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG35860.22455488225271253No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA30080.1883605928098604No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG25160.15755161286888586No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20170.1263042937824097No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA19250.12054326501295919No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCGG400.001931514723.12531
GGTATCA49100.021.5142571
TTTACCG500.00703605218.530
ACCGTTA2300.018.4999988
TATACCG1156.409573E-817.695655
ATACGTT752.067902E-417.2666666
GTATTGG7050.016.7943271
GACCGTT2500.016.2799997
TTAACGG1151.2428609E-616.08695635
TATCGTT1059.347574E-615.85714413
CCGTTAT2850.015.5789489
TTAGGGT1557.214112E-915.5161294
TTATGCG1557.214112E-915.5161294
AAGACCG1201.9365507E-615.4166665
CCGTTTA2650.015.35849127
TCCTACA2300.015.2826072
CGTATAC855.365774E-415.2352943
GTTATCG1101.4524927E-515.13636411
GGCTAGC1001.09378714E-414.81
CGCCGTT3050.014.55737725
Produced by FastQC (version 0.11.5)