##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088577_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1596937
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32855522791444	31.0	31.0	33.0	30.0	34.0
2	31.523463355160537	31.0	31.0	34.0	30.0	34.0
3	31.584716867352938	31.0	31.0	34.0	30.0	34.0
4	35.32414741470703	37.0	35.0	37.0	33.0	37.0
5	35.20790613530778	37.0	35.0	37.0	33.0	37.0
6	35.26518829484194	37.0	35.0	37.0	32.0	37.0
7	35.12395730075764	37.0	35.0	37.0	32.0	37.0
8	35.14668268065678	37.0	35.0	37.0	32.0	37.0
9	36.69278437408614	39.0	35.0	39.0	32.0	39.0
10	36.496373369769756	38.0	35.0	39.0	32.0	39.0
11	36.576750366482834	38.0	35.0	39.0	32.0	39.0
12	36.443917950426346	38.0	35.0	39.0	32.0	39.0
13	36.51206090158848	38.0	35.0	39.0	32.0	39.0
14	37.41731201669195	39.0	36.0	41.0	32.0	41.0
15	37.39097847942655	39.0	36.0	41.0	32.0	41.0
16	37.27715808450803	39.0	36.0	40.0	32.0	41.0
17	37.31121828851107	39.0	36.0	40.0	32.0	41.0
18	37.287420856301786	39.0	36.0	40.0	32.0	41.0
19	37.31718283188379	39.0	36.0	40.0	31.0	41.0
20	37.18970692018533	39.0	36.0	40.0	31.0	41.0
21	37.08332889775865	39.0	36.0	40.0	31.0	41.0
22	36.922200437462465	39.0	36.0	40.0	31.0	41.0
23	36.77529983963049	39.0	35.0	40.0	30.0	41.0
24	36.62975934554713	39.0	35.0	40.0	30.0	41.0
25	36.41809852235874	38.0	35.0	40.0	30.0	41.0
26	36.55883857660008	39.0	35.0	40.0	30.0	41.0
27	36.46428757051781	38.0	35.0	40.0	30.0	41.0
28	36.341285222898584	38.0	35.0	40.0	30.0	41.0
29	36.17906028853987	38.0	35.0	40.0	29.0	41.0
30	36.069769815590725	38.0	35.0	40.0	29.0	41.0
31	35.869036787299684	38.0	35.0	40.0	29.0	41.0
32	35.75268717551162	38.0	34.0	40.0	28.0	41.0
33	35.54441471391796	38.0	34.0	40.0	27.0	41.0
34	35.37984403893203	38.0	34.0	40.0	27.0	41.0
35	35.185958494292514	38.0	34.0	40.0	26.0	41.0
36	35.052509898637204	38.0	34.0	40.0	25.0	41.0
37	34.880118000897966	38.0	33.0	40.0	25.0	41.0
38	34.782670825461494	38.0	33.0	40.0	24.0	41.0
39	34.63734198656553	38.0	33.0	40.0	24.0	41.0
40	34.45070782379017	38.0	33.0	40.0	23.0	41.0
41	34.32015978088052	38.0	33.0	40.0	23.0	41.0
42	34.08490441388734	38.0	33.0	40.0	21.0	41.0
43	33.71961636557986	37.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	1.0
13	5.0
14	9.0
15	20.0
16	28.0
17	110.0
18	186.0
19	443.0
20	900.0
21	1707.0
22	3053.0
23	5244.0
24	8301.0
25	12128.0
26	17526.0
27	23501.0
28	31404.0
29	40582.0
30	49901.0
31	60781.0
32	73896.0
33	89337.0
34	108326.0
35	131837.0
36	170607.0
37	232204.0
38	307024.0
39	227873.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.43891524837861	22.437578940183613	14.343333519105638	22.780172292332132
2	19.744548469977214	24.117357165623943	33.867460018773436	22.270634345625407
3	20.66975716637538	24.999608625762946	32.2417853678636	22.08884883999807
4	14.630132560019588	17.002298775718767	35.98551476983751	32.38205389442414
5	12.913283366845404	39.00836413709495	34.539872267972996	13.538480228086645
6	32.471099360838906	37.83849957762892	13.610430467826845	16.079970593705326
7	26.08117915734935	32.87161610007158	21.657836220214072	19.389368522365004
8	27.201073054228186	34.73192743358066	18.970504158899193	19.09649535329196
9	24.728026215185697	14.203315472056818	21.4927076021158	39.575950710641685
10	16.319429006905096	27.699464662663587	33.62956710252189	22.351539227909427
11	33.706777412008115	22.360180771063604	23.339931381137767	20.593110435790514
12	20.792116407848273	27.095182840650573	31.245002150992807	20.867698600508348
13	31.502557708914004	20.80877329537734	26.368353917530875	21.32031507817779
14	20.532619633711285	22.504331730055725	27.788948468223857	29.174100168009133
15	23.012679899081807	29.616071266430673	24.99998434503052	22.371264489457005
16	22.02754397950577	28.116199950279817	26.463097792837164	23.393158277377253
17	21.946451237587958	26.670682688171166	27.28091339858742	24.101952675653454
18	22.929645940948205	25.445462156616074	29.459145852341074	22.16574605009465
19	22.896958364669366	24.900105639734065	30.37352130985756	21.829414685739014
20	23.729176542343247	24.629149427936106	30.07770500652186	21.563969023198787
21	22.85155895317098	25.324605792213468	30.392182033480342	21.43165322113521
22	21.67850077993058	25.72111485925869	30.136943411042516	22.463440949768213
23	22.44578214419229	25.924128503503894	30.798960760505896	20.831128591797924
24	22.48786270216045	25.918367474734445	28.838207142798993	22.75556268030611
25	22.561127959337156	25.934961742385582	29.605238027548992	21.898672270728277
26	22.358552654237457	26.578130508592384	29.62145657593255	21.441860261237608
27	21.436161852346086	26.675379179015827	29.465470460011883	22.4229885086262
28	21.72014299875324	26.40003957576285	29.55376448789151	22.326052937592404
29	21.97049727071262	26.293711023039734	29.15938449669586	22.576407209551785
30	21.920777087637145	26.504051193002603	30.27771289662648	21.29745882273377
31	22.392868347342443	26.193206118963992	30.30714423925302	21.106781294440545
32	22.04144559240596	26.06114079641213	29.358202609119832	22.539211002062075
33	21.814573774669885	26.123698054463013	29.827789073707976	22.233939097159126
34	21.254501586474607	26.62440659838178	30.203383101524985	21.917708713618634
35	20.90439384897463	28.282643585814593	29.429338790446963	21.383623774763812
36	22.692128743964226	26.293084824260443	29.333843476605526	21.6809429551698
37	21.828663247203867	26.82629308482426	29.319065185414328	22.025978482557544
38	21.759029942947027	25.57239264917777	30.438395503391806	22.230181904483395
39	21.219872793979974	25.535572160955628	31.034536741274078	22.210018303790317
40	20.477827240523578	26.23653907449073	30.626505616689954	22.659128068295743
41	21.518319132188683	25.15979027350484	30.71918303602459	22.602707558281885
42	20.500433016455876	26.808008080469047	30.121037962048597	22.57052094102648
43	20.691611503772535	26.63323600116974	29.550946593384708	23.124205901673015
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	131.0
1	210.0
2	289.0
3	559.5
4	830.0
5	830.0
6	1623.5
7	2417.0
8	2779.0
9	3141.0
10	4582.5
11	6024.0
12	6024.0
13	11366.0
14	16708.0
15	27338.0
16	37968.0
17	34272.0
18	30576.0
19	30576.0
20	33225.5
21	35875.0
22	29205.0
23	22535.0
24	25251.0
25	27967.0
26	27967.0
27	30980.5
28	33994.0
29	37770.5
30	41547.0
31	44974.5
32	48402.0
33	48402.0
34	53646.0
35	58890.0
36	64599.0
37	70308.0
38	74841.5
39	79375.0
40	79375.0
41	82574.0
42	85773.0
43	90971.5
44	96170.0
45	101162.5
46	106155.0
47	106155.0
48	131290.5
49	156426.0
50	148583.5
51	140741.0
52	131443.5
53	122146.0
54	122146.0
55	109440.0
56	96734.0
57	84579.5
58	72425.0
59	65099.5
60	57774.0
61	57774.0
62	51141.0
63	44508.0
64	38561.5
65	32615.0
66	27786.0
67	22957.0
68	22957.0
69	19834.0
70	16711.0
71	14013.0
72	11315.0
73	9411.5
74	7508.0
75	7508.0
76	5947.5
77	4387.0
78	3501.5
79	2616.0
80	2020.0
81	1424.0
82	1424.0
83	1067.0
84	710.0
85	567.0
86	424.0
87	310.5
88	197.0
89	197.0
90	173.5
91	150.0
92	98.5
93	47.0
94	41.5
95	36.0
96	36.0
97	22.5
98	9.0
99	5.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1596937.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.17171007948788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.45306299744118	45.49076486860575
2	10.135334217932595	11.183674420609805
3	2.9719844653481235	4.919083958487853
4	1.2843082812816233	2.8342993659022038
5	0.7116369471554375	1.9631113665155815
6	0.4513389976483609	1.4940686595493216
7	0.3176329058858121	1.22670454166661
8	0.2319281127872025	1.0236696478382643
9	0.16927010933162062	0.8405029257450649
>10	1.1070638130450274	11.643032106937977
>50	0.10177447285017992	3.924737090691324
>100	0.05761875559198352	5.798822845751879
>500	0.003977537573054152	1.5000807512067753
>1k	0.0026138104051498714	2.776011505106791
>5k	1.1364393065869006E-4	0.4227186802355728
>10k+	3.4093179197607017E-4	2.9587172651492795
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19530	1.2229662159496586	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15509	0.9711716867979137	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12150	0.7608315168350411	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6742	0.4221832169960368	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3862	0.24183796856106407	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3753	0.2350124018668238	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3586	0.22455488225271253	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3586	0.22455488225271253	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3008	0.1883605928098604	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2516	0.15755161286888586	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2017	0.1263042937824097	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1925	0.12054326501295919	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	6.261987792880996E-5	0.0	0.0	0.0	0.0
4	6.261987792880996E-5	0.0	0.0	0.0	0.0
5	6.261987792880996E-5	0.0	0.0	0.0	0.0
6	6.261987792880996E-5	0.0	0.0	0.0	0.0
7	6.261987792880996E-5	0.0	0.0	0.0	0.0
8	6.261987792880996E-5	0.0	0.0	0.0	0.0
9	1.2523975585761993E-4	0.0	0.0	1.878596337864299E-4	0.0
10	1.2523975585761993E-4	0.0	0.0	2.5047951171523985E-4	0.0
11	1.2523975585761993E-4	0.0	0.0	4.3833914550166973E-4	0.0
12	1.2523975585761993E-4	0.0	0.0	6.261987792880997E-4	0.0
13	1.2523975585761993E-4	0.0	0.0	6.888186572169096E-4	0.0
14	1.2523975585761993E-4	0.0	0.0	7.514385351457196E-4	0.0
15	1.2523975585761993E-4	0.0	0.0	0.0010019180468609594	0.0
16	1.2523975585761993E-4	0.0	0.0	0.0013776373144338192	0.0
17	1.2523975585761993E-4	0.0	0.0	0.002003836093721919	0.0
18	1.2523975585761993E-4	0.0	0.0	0.0023795553612947786	0.0
19	1.2523975585761993E-4	0.0	0.0	0.003256233652298118	0.0
20	1.2523975585761993E-4	0.0	6.261987792880996E-5	0.0058862685253081365	0.0
21	1.2523975585761993E-4	0.0	6.261987792880996E-5	0.010457519614111264	0.0
22	1.878596337864299E-4	0.0	6.261987792880996E-5	0.0182223844772837	0.0
23	1.878596337864299E-4	0.0	6.261987792880996E-5	0.02216743678679873	0.0
24	2.5047951171523985E-4	0.0	6.261987792880996E-5	0.030746360063045694	0.0
25	2.5047951171523985E-4	0.0	6.261987792880996E-5	0.0364447689545674	0.0
26	2.5047951171523985E-4	0.0	6.261987792880996E-5	0.044084394061882214	0.0
27	2.5047951171523985E-4	0.0	6.261987792880996E-5	0.08297133825567321	0.0
28	2.5047951171523985E-4	0.0	6.261987792880996E-5	0.174646839543451	0.0
29	2.5047951171523985E-4	0.0	6.261987792880996E-5	0.29725656052806093	0.0
30	2.5047951171523985E-4	0.0	6.261987792880996E-5	0.46157112021325825	0.0
31	2.5047951171523985E-4	0.0	6.261987792880996E-5	0.6916365517237061	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACCGG	40	0.0019315147	23.125	31
GGTATCA	4910	0.0	21.514257	1
TTTACCG	50	0.007036052	18.5	30
ACCGTTA	230	0.0	18.499998	8
TATACCG	115	6.409573E-8	17.69565	5
ATACGTT	75	2.067902E-4	17.266666	6
GTATTGG	705	0.0	16.794327	1
GACCGTT	250	0.0	16.279999	7
TTAACGG	115	1.2428609E-6	16.086956	35
TATCGTT	105	9.347574E-6	15.857144	13
CCGTTAT	285	0.0	15.578948	9
TTAGGGT	155	7.214112E-9	15.516129	4
TTATGCG	155	7.214112E-9	15.516129	4
AAGACCG	120	1.9365507E-6	15.416666	5
CCGTTTA	265	0.0	15.358491	27
TCCTACA	230	0.0	15.282607	2
CGTATAC	85	5.365774E-4	15.235294	3
GTTATCG	110	1.4524927E-5	15.136364	11
GGCTAGC	100	1.09378714E-4	14.8	1
CGCCGTT	305	0.0	14.557377	25
>>END_MODULE