FastQCFastQC Report
Wed 25 May 2016
SRR2088576_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088576_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2749988
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT255570.9293495098887704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT212370.7722579153072667No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT161470.5871661985434118No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90170.3278923398938468No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG49380.17956441991746872No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA46830.17029165218175496No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA46600.16945528489578865No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG42010.15276430297150387No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA38440.13978242814150463No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG30750.1118186697541953No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG400.001931867423.12527
GGTATCA66900.022.371451
CGTAGAC1151.2436576E-616.0869563
GTATCAA100150.014.9625562
AGTGCGC2850.013.6315788
ACAGTCC3200.013.2968758
GTATTGG9800.013.2142871
TAATACT6750.013.1555554
TAGTACT4950.013.0808094
TAGACTG2703.6379788E-1213.0185185
CGAACTA3300.012.89393924
ATAGTAC4200.012.7738093
TTAACGG2204.090907E-912.61363635
ACGTTTA1359.9698074E-512.33333426
TAAACGT1501.9457493E-512.3333334
GTTATCG1653.8083908E-612.33333311
AACGCAG154350.012.1655335
CTAATAC6700.012.1492543
GTACTAC2901.2732926E-1112.1206891
CTTATAG2603.092282E-1012.0961543
Produced by FastQC (version 0.11.5)