##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088576_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2749988
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32739415590177	31.0	31.0	33.0	30.0	34.0
2	31.52853466996947	31.0	31.0	34.0	30.0	34.0
3	31.596557148613012	31.0	31.0	34.0	30.0	34.0
4	35.34710078734889	37.0	35.0	37.0	33.0	37.0
5	35.206861266303704	37.0	35.0	37.0	33.0	37.0
6	35.25661166521454	37.0	35.0	37.0	32.0	37.0
7	35.131108208472185	37.0	35.0	37.0	32.0	37.0
8	35.14008352036445	37.0	35.0	37.0	32.0	37.0
9	36.70120342343312	39.0	35.0	39.0	32.0	39.0
10	36.489555227150085	38.0	35.0	39.0	32.0	39.0
11	36.572973409338516	38.0	35.0	39.0	32.0	39.0
12	36.44362339035661	38.0	35.0	39.0	32.0	39.0
13	36.51050913676714	38.0	35.0	39.0	32.0	39.0
14	37.45930382241668	39.0	36.0	41.0	32.0	41.0
15	37.4185985538846	39.0	36.0	41.0	32.0	41.0
16	37.306035517245896	39.0	36.0	40.0	32.0	41.0
17	37.31191263380058	39.0	36.0	40.0	32.0	41.0
18	37.2719350048073	39.0	36.0	40.0	32.0	41.0
19	37.293121279074676	39.0	36.0	40.0	31.0	41.0
20	37.152531211045286	39.0	36.0	40.0	31.0	41.0
21	37.04283255054204	39.0	36.0	40.0	31.0	41.0
22	36.8826762153144	39.0	36.0	40.0	30.0	41.0
23	36.74668580371987	39.0	35.0	40.0	30.0	41.0
24	36.597756062935545	38.0	35.0	40.0	30.0	41.0
25	36.398574102868814	38.0	35.0	40.0	30.0	41.0
26	36.55168931646247	39.0	35.0	40.0	30.0	41.0
27	36.4575463602023	39.0	35.0	40.0	30.0	41.0
28	36.34029748493448	38.0	35.0	40.0	30.0	41.0
29	36.17990842141857	38.0	35.0	40.0	29.0	41.0
30	36.087974929345144	38.0	35.0	40.0	29.0	41.0
31	35.9222312970093	38.0	35.0	40.0	29.0	41.0
32	35.80637151871208	38.0	34.0	40.0	28.0	41.0
33	35.617364875774	38.0	34.0	40.0	27.0	41.0
34	35.48159301058768	38.0	34.0	40.0	27.0	41.0
35	35.302255864389224	38.0	34.0	40.0	26.0	41.0
36	35.1772560462082	38.0	34.0	40.0	26.0	41.0
37	35.02027754302928	38.0	33.0	40.0	25.0	41.0
38	34.95290088538568	38.0	33.0	40.0	25.0	41.0
39	34.823363592859316	38.0	33.0	40.0	24.0	41.0
40	34.663930169877105	38.0	33.0	40.0	24.0	41.0
41	34.553874416906545	38.0	33.0	40.0	23.0	41.0
42	34.35425063673005	38.0	33.0	40.0	23.0	41.0
43	33.9920796018019	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	4.0
13	10.0
14	13.0
15	27.0
16	75.0
17	162.0
18	349.0
19	780.0
20	1545.0
21	2991.0
22	5466.0
23	8652.0
24	13979.0
25	20406.0
26	29041.0
27	39513.0
28	52814.0
29	67679.0
30	84940.0
31	103641.0
32	126025.0
33	151706.0
34	184969.0
35	227243.0
36	291533.0
37	396260.0
38	542393.0
39	397768.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.393630808570805	22.159369422702934	13.928751689098279	23.518248079627984
2	20.024160105425914	23.671557839525118	33.26447242678877	23.039809628260198
3	20.76587243289789	24.83210835829102	31.590392394439537	22.811626814371554
4	15.135956956903085	17.026983390472978	35.512118598335704	32.32494105428824
5	13.403585761101503	38.37864019770268	34.53582342904769	13.681950612148125
6	33.10818083569819	37.45034523787013	13.839987665400722	15.601486261030958
7	26.8456807811525	32.28283177962958	21.63223984977389	19.239247589444027
8	27.28630088567659	34.611714669300376	18.960191826291606	19.141792618731426
9	25.213637295871838	14.094243320334488	20.807763524786292	39.884355859007385
10	16.3704350709894	27.461828924344395	33.695056123881265	22.472679880784934
11	34.61236921761113	22.11704196527403	22.80017221893332	20.470416598181522
12	20.86430922607662	26.532770324815964	30.880462023834287	21.72245842527313
13	31.358245926891314	20.5606715374758	25.782367050328947	22.298715485303937
14	20.966746036709978	21.985914120352525	27.07989998501812	29.96743985791938
15	23.83959493641427	29.16878182741161	24.075777785212154	22.915845450961967
16	22.89169989105407	27.676993499608	25.74651234841752	23.684794260920413
17	22.474970799872583	26.546988568677392	26.742007601487717	24.23603302996231
18	23.203628524924472	25.445965582395267	28.47674244396703	22.87366344871323
19	23.417047638026055	24.98265446976496	29.19729104272455	22.403006849484434
20	24.06814138825333	24.833490182502615	28.93921718931137	22.159151239932683
21	23.468102406265047	25.216255489114864	29.053254050563133	22.262388054056963
22	22.55991662509073	25.626402733393743	28.869653249396	22.94402739211953
23	23.003482197013223	25.67138474786072	29.392673713485294	21.932459341640765
24	23.122937263726243	25.75811239903592	27.82470323506866	23.294247102169173
25	23.261119684885898	25.70847581880357	28.442924114577956	22.587480381732576
26	23.028755034567425	26.144368629972202	28.513433513164422	22.31344282229595
27	22.4525707021267	26.244405430132783	28.296305292968547	23.006718574771963
28	22.60235317390476	26.147495916345814	28.39845119324157	22.851699716507852
29	22.7579174890945	26.139714064206824	28.130959116912514	22.971409329786166
30	22.65882614760501	26.199423415665812	29.07609051384952	22.065659922879664
31	23.064137007143305	25.890403885398772	29.02099936436086	22.02445974309706
32	22.697444497939628	25.833749092723313	28.302305319150484	23.166501090186575
33	22.58824402142846	25.915458540182723	28.75245273797558	22.743844700413238
34	22.265406249045448	26.11862306308246	28.999908363236493	22.6160623246356
35	21.97609589569118	27.424737853401542	28.49557888979879	22.103587361108485
36	23.328210886738415	25.913822169405833	28.358378291105268	22.399588652750484
37	22.643116988146858	26.238187221180603	28.33656001408006	22.78213577659248
38	22.53017104074636	25.35214699118687	29.29783693601572	22.81984503205105
39	22.137078416342177	25.228146450093604	29.815984651569387	22.81879048199483
40	21.586712378381286	25.775494293066004	29.503583288363437	23.134210040189267
41	22.275806294427465	24.840581122535806	29.67394766813528	23.209664914901445
42	21.469620958346	26.198405229404635	29.225509347677153	23.10646446457221
43	21.58998511993507	25.913894896995913	28.843180406605413	23.652939576463606
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	156.0
1	271.0
2	386.0
3	811.5
4	1237.0
5	1237.0
6	2253.0
7	3269.0
8	3649.0
9	4029.0
10	6071.0
11	8113.0
12	8113.0
13	15384.0
14	22655.0
15	36449.0
16	50243.0
17	45844.0
18	41445.0
19	41445.0
20	45717.0
21	49989.0
22	41757.0
23	33525.0
24	38902.0
25	44279.0
26	44279.0
27	49366.5
28	54454.0
29	60784.0
30	67114.0
31	74258.5
32	81403.0
33	81403.0
34	90790.0
35	100177.0
36	110361.5
37	120546.0
38	130047.0
39	139548.0
40	139548.0
41	145438.5
42	151329.0
43	159960.5
44	168592.0
45	177016.5
46	185441.0
47	185441.0
48	218375.5
49	251310.0
50	243180.5
51	235051.0
52	223475.5
53	211900.0
54	211900.0
55	193098.0
56	174296.0
57	157264.5
58	140233.0
59	126763.0
60	113293.0
61	113293.0
62	101401.0
63	89509.0
64	77765.5
65	66022.0
66	56672.5
67	47323.0
68	47323.0
69	40633.5
70	33944.0
71	28700.5
72	23457.0
73	19268.0
74	15079.0
75	15079.0
76	12076.5
77	9074.0
78	7089.5
79	5105.0
80	3988.5
81	2872.0
82	2872.0
83	2250.5
84	1629.0
85	1263.0
86	897.0
87	677.0
88	457.0
89	457.0
90	428.5
91	400.0
92	265.5
93	131.0
94	91.5
95	52.0
96	52.0
97	34.0
98	16.0
99	12.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2749988.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.080786207542175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.90068293474602	41.6129038576112
2	11.234732411963432	11.702273936928242
3	3.6259971912810185	5.665343535247653
4	1.5459676680826064	3.2206084642073094
5	0.8675652225802892	2.259173943915139
6	0.49968027447883595	1.5614244928354943
7	0.35911221489177114	1.3091992541806692
8	0.26189784528402027	1.0911876550762412
9	0.1956036905653891	0.9168474590768037
>10	1.308625258475174	12.97366563927597
>50	0.12275299102769888	4.45709034511302
>100	0.07044869364921855	6.607679399916308
>500	0.004552365589455215	1.6632465279612219
>1k	0.0021010918105107915	2.334651138586883
>5k	7.003639368369304E-5	0.32889962373274256
>10k+	2.1010918105107912E-4	2.295804726334984
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	25557	0.9293495098887704	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21237	0.7722579153072667	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16147	0.5871661985434118	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9017	0.3278923398938468	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4938	0.17956441991746872	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4683	0.17029165218175496	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4660	0.16945528489578865	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4201	0.15276430297150387	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3844	0.13978242814150463	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3075	0.1118186697541953	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	7.272759008402946E-5	0.0	0.0	0.0	0.0
7	7.272759008402946E-5	0.0	0.0	0.0	0.0
8	1.0909138512604419E-4	0.0	0.0	0.0	0.0
9	1.0909138512604419E-4	0.0	0.0	3.636379504201473E-5	0.0
10	1.0909138512604419E-4	0.0	0.0	3.636379504201473E-5	0.0
11	1.0909138512604419E-4	0.0	0.0	1.0909138512604419E-4	0.0
12	1.0909138512604419E-4	0.0	0.0	1.0909138512604419E-4	0.0
13	1.0909138512604419E-4	0.0	0.0	1.0909138512604419E-4	0.0
14	1.0909138512604419E-4	0.0	0.0	1.4545518016805892E-4	0.0
15	1.0909138512604419E-4	0.0	0.0	2.9091036033611784E-4	0.0
16	1.0909138512604419E-4	0.0	0.0	6.181845157142504E-4	0.0
17	1.0909138512604419E-4	0.0	0.0	9.45458671092383E-4	0.0
18	1.4545518016805892E-4	0.0	0.0	0.0013090966215125303	0.0
19	1.4545518016805892E-4	0.0	0.0	0.0017818259570587217	0.0
20	1.4545518016805892E-4	0.0	0.0	0.0024727380628570017	0.0
21	1.4545518016805892E-4	0.0	0.0	0.0040000174546216205	0.0
22	1.4545518016805892E-4	0.0	0.0	0.0070909400331928725	0.0
23	1.4545518016805892E-4	0.0	0.0	0.008581855629915477	0.0
24	1.4545518016805892E-4	0.0	0.0	0.011672778208486728	0.0
25	1.4545518016805892E-4	0.0	0.0	0.01461824560688992	0.0
26	1.4545518016805892E-4	0.0	0.0	0.018981901011931687	0.0
27	1.4545518016805892E-4	0.0	0.0	0.049309306076971975	0.0
28	1.4545518016805892E-4	0.0	0.0	0.11738233039562354	0.0
29	1.4545518016805892E-4	0.0	0.0	0.20396452639066062	0.0
30	1.8181897521007365E-4	0.0	0.0	0.32007412395981366	0.0
31	2.1818277025208838E-4	0.0	0.0	0.4926930590242576	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATACG	40	0.0019318674	23.125	27
GGTATCA	6690	0.0	22.37145	1
CGTAGAC	115	1.2436576E-6	16.086956	3
GTATCAA	10015	0.0	14.962556	2
AGTGCGC	285	0.0	13.631578	8
ACAGTCC	320	0.0	13.296875	8
GTATTGG	980	0.0	13.214287	1
TAATACT	675	0.0	13.155555	4
TAGTACT	495	0.0	13.080809	4
TAGACTG	270	3.6379788E-12	13.018518	5
CGAACTA	330	0.0	12.893939	24
ATAGTAC	420	0.0	12.773809	3
TTAACGG	220	4.090907E-9	12.613636	35
ACGTTTA	135	9.9698074E-5	12.333334	26
TAAACGT	150	1.9457493E-5	12.333333	4
GTTATCG	165	3.8083908E-6	12.333333	11
AACGCAG	15435	0.0	12.165533	5
CTAATAC	670	0.0	12.149254	3
GTACTAC	290	1.2732926E-11	12.120689	1
CTTATAG	260	3.092282E-10	12.096154	3
>>END_MODULE