FastQCFastQC Report
Wed 25 May 2016
SRR2088575_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088575_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2940064
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT370721.260924932246373No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT312551.0630720963897384No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT258610.8796067024391306No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131980.44890179261403834No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG89010.3027485115970264No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA78380.2665928360743168No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA77850.26479015422793517No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG77500.2635997039520228No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA68610.23336226694384887No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG58950.20050583932866767No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA42480.14448665063073457No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT36650.12465715032053724No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA35420.12047356792233095No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG35210.11975929775678353No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC34420.11707228141972419No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC33830.11506552238318621No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA32450.1103717470095889No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32240.1096574768440415No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31160.10598408742122621No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC29910.10173247929296776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96450.022.7102131
TATACCG1355.638867E-1119.1851845
GTATTGG15750.018.558731
ATTGGAC17200.015.9186063
TAATACT7300.015.4589044
TTGGACC21200.015.0966994
TGGACCC19850.015.0050385
GTATCAA150800.014.5006642
TAGTACT5300.014.3113214
TATTGGA19650.014.3104332
TATACTG3650.014.1917815
TTATGCG3400.014.14705854
TGCGCTA1051.657016E-414.0952385
GGACCCT22250.013.9685396
GTATTAG6250.013.9121
GTTTCGC800.006301698713.87500132
TCTAATA7200.013.8750012
CCGTTTA3600.013.87500127
CTCGTCT5150.013.65048613
CTCTAAT6150.013.5365851
Produced by FastQC (version 0.11.5)