##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088575_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2940064
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.524523275683794	31.0	31.0	34.0	30.0	34.0
2	31.70104222221013	31.0	31.0	34.0	30.0	34.0
3	31.779914654919075	31.0	31.0	34.0	30.0	34.0
4	35.48250684338844	37.0	35.0	37.0	33.0	37.0
5	35.384347075437816	37.0	35.0	37.0	33.0	37.0
6	35.42703934336123	37.0	35.0	37.0	33.0	37.0
7	35.29246982378615	37.0	35.0	37.0	32.0	37.0
8	35.317564175473734	37.0	35.0	37.0	32.0	37.0
9	36.89070952196959	39.0	37.0	39.0	33.0	39.0
10	36.739306015107154	39.0	37.0	39.0	32.0	39.0
11	36.789200847328495	39.0	37.0	39.0	32.0	39.0
12	36.68453679919893	39.0	35.0	39.0	32.0	39.0
13	36.74222329854044	39.0	35.0	39.0	32.0	39.0
14	37.719306110343176	40.0	37.0	41.0	32.0	41.0
15	37.67917739205677	39.0	37.0	41.0	32.0	41.0
16	37.57634459658021	39.0	36.0	41.0	32.0	41.0
17	37.61124553751211	39.0	36.0	41.0	32.0	41.0
18	37.59256703255439	39.0	36.0	41.0	32.0	41.0
19	37.62541393656737	39.0	37.0	41.0	32.0	41.0
20	37.499219064619	39.0	36.0	41.0	32.0	41.0
21	37.3959427413825	39.0	36.0	41.0	31.0	41.0
22	37.23862065587688	39.0	36.0	41.0	31.0	41.0
23	37.11664337919174	39.0	36.0	40.0	31.0	41.0
24	36.97207033588384	39.0	36.0	40.0	31.0	41.0
25	36.77665350142038	39.0	35.0	40.0	30.0	41.0
26	36.92541488892759	39.0	36.0	40.0	31.0	41.0
27	36.838440931898084	39.0	35.0	40.0	30.0	41.0
28	36.71795784037354	39.0	35.0	40.0	30.0	41.0
29	36.56566149580417	39.0	35.0	40.0	30.0	41.0
30	36.444681136192955	39.0	35.0	40.0	30.0	41.0
31	36.25396317903284	38.0	35.0	40.0	30.0	41.0
32	36.1361558795999	38.0	35.0	40.0	30.0	41.0
33	35.92988247874876	38.0	35.0	40.0	29.0	41.0
34	35.78241494062714	38.0	35.0	40.0	28.0	41.0
35	35.573588193998496	38.0	34.0	40.0	27.0	41.0
36	35.42156803389314	38.0	34.0	40.0	26.0	41.0
37	35.25030475527063	38.0	34.0	40.0	26.0	41.0
38	35.15280993883126	38.0	34.0	40.0	25.0	41.0
39	34.99888029648334	38.0	34.0	40.0	24.0	41.0
40	34.809818425721346	38.0	33.0	40.0	24.0	41.0
41	34.67479143311166	38.0	33.0	40.0	23.0	41.0
42	34.44313253044832	38.0	33.0	40.0	22.0	41.0
43	34.072004895131535	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	2.0
13	5.0
14	16.0
15	9.0
16	52.0
17	124.0
18	262.0
19	587.0
20	1253.0
21	2433.0
22	4535.0
23	7875.0
24	12619.0
25	19193.0
26	27642.0
27	38593.0
28	52112.0
29	67955.0
30	84544.0
31	103536.0
32	125289.0
33	152505.0
34	186923.0
35	228324.0
36	295412.0
37	414883.0
38	561621.0
39	551758.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.406853728354214	21.599291716098698	13.978267139762943	23.015587415784147
2	19.090978971886326	23.59054088618479	34.70036706683936	22.61811307508952
3	20.128949573886825	24.938436714302817	32.40238987994819	22.530223831862166
4	13.968505447500462	16.347739368938907	36.31410744800113	33.3696477355595
5	12.7516952011929	38.71147022649847	34.771760070529076	13.765074501779553
6	32.29150793996321	37.38996838164067	13.668852106620808	16.64967157177531
7	26.1096016957454	32.82394532908127	21.759152181721213	19.307300793452114
8	27.205972386995658	34.31469519030878	19.043122870794647	19.43620955190091
9	24.56347889025545	14.203704409155717	21.842211598114872	39.390605102473955
10	16.37494966095976	27.273147795422144	33.62341772151899	22.72848482209911
11	33.21305250497949	22.198870500778213	23.753258432469497	20.834818561772806
12	20.914544717393905	26.90472044145978	31.19867458667566	20.98206025447065
13	31.523089293294294	20.50707059438162	26.645712474286277	21.32412763803781
14	20.527002133286896	22.438253044831676	27.92894984598975	29.10579497589168
15	22.896474362462857	29.188106109254765	25.581824069135912	22.33359545914647
16	21.868231439859812	28.015546600346113	26.7008133156285	23.41540864416557
17	21.83469475494411	26.222014214656554	27.338486509137216	24.60480452126212
18	23.002526475614136	25.137071846055054	29.751903359926857	22.108498318403953
19	22.87749518377831	24.310865341706847	30.754228479380043	22.0574109951348
20	23.814209486596212	24.079679898124667	30.584674347225093	21.52143626805403
21	22.813959151909618	24.8717374859867	31.007624323824245	21.306679038279437
22	21.451301740370276	25.272477061723826	30.75433051797512	22.521890679930777
23	22.28063742830088	25.50682570175343	31.645059427277772	20.567477442667915
24	22.421314638048695	25.46097635969829	29.207731532374805	22.909977469878207
25	22.35597592433362	25.68818229807242	30.07349499874833	21.88234677884563
26	22.150776309631354	26.35704528881004	30.13485420725535	21.357324194303253
27	21.058283084994066	26.568095116296785	29.823194325021497	22.550427473687648
28	21.561605461649812	26.07001072085506	30.02907419702428	22.339309620470846
29	21.840885096378855	25.99018252663888	29.44561751036712	22.723314866615148
30	21.752383621581025	26.12657411539341	30.93456468974825	21.186477573277315
31	22.447096389738455	25.71736533626479	30.838682423239767	20.99685585075699
32	22.030404780304103	25.595871382391678	29.793263003798558	22.580460833505665
33	21.6966365358033	25.800118636873208	30.348659076809213	22.154585750514276
34	21.107533713551813	26.366704942477444	30.808751102016828	21.717010241953915
35	20.807948398402214	28.29390788771945	29.627620351121607	21.27052336275673
36	22.684166058970145	25.988005703277206	29.676700915354225	21.651127322398427
37	21.85010258280092	26.57112226128411	29.57527455184649	22.00350060406848
38	21.621434091230668	25.267239080509814	30.80276483777224	22.308561990487284
39	21.111411180164787	25.135337189938724	31.490504968599325	22.262746661297168
40	20.230682053179795	25.93382320929068	30.96925781207484	22.866236925454682
41	21.510041958270296	24.8750027210292	30.976638603785496	22.638316716915003
42	20.28564684306192	26.78254623028614	30.409372040880744	22.522434885771194
43	20.62941486988038	26.58928513120803	29.610375828553394	23.170924170358195
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	162.0
1	382.0
2	602.0
3	1508.0
4	2414.0
5	2414.0
6	3908.5
7	5403.0
8	5954.5
9	6506.0
10	9398.0
11	12290.0
12	12290.0
13	22685.5
14	33081.0
15	52753.0
16	72425.0
17	66409.0
18	60393.0
19	60393.0
20	64322.5
21	68252.0
22	53928.0
23	39604.0
24	44054.5
25	48505.0
26	48505.0
27	52438.5
28	56372.0
29	62036.5
30	67701.0
31	75126.0
32	82551.0
33	82551.0
34	92722.5
35	102894.0
36	113869.5
37	124845.0
38	133830.0
39	142815.0
40	142815.0
41	148593.5
42	154372.0
43	166074.0
44	177776.0
45	189694.5
46	201613.0
47	201613.0
48	255900.5
49	310188.0
50	291521.0
51	272854.0
52	252479.0
53	232104.0
54	232104.0
55	205838.0
56	179572.0
57	154212.0
58	128852.0
59	114331.0
60	99810.0
61	99810.0
62	88308.0
63	76806.0
64	66666.5
65	56527.0
66	48585.5
67	40644.0
68	40644.0
69	35028.5
70	29413.0
71	25341.0
72	21269.0
73	17475.5
74	13682.0
75	13682.0
76	10763.0
77	7844.0
78	6141.5
79	4439.0
80	3504.0
81	2569.0
82	2569.0
83	1936.0
84	1303.0
85	1007.5
86	712.0
87	565.0
88	418.0
89	418.0
90	376.0
91	334.0
92	212.5
93	91.0
94	65.0
95	39.0
96	39.0
97	24.5
98	10.0
99	9.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2940064.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.56457409971916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.70964005668483	35.86371226725039
2	11.54368192966703	10.519659013358048
3	3.847286411345093	5.258999003177283
4	1.6806453372721328	3.0631155602193436
5	0.9293681645241273	2.117313229918979
6	0.5845065048288604	1.5979673970625476
7	0.4043520346513522	1.2896889771670625
8	0.2966380063727569	1.0812947537731556
9	0.22885671935434498	0.9384983052515715
>10	1.504493420944216	13.33606428259381
>50	0.1581116641916028	4.99009373676783
>100	0.09964489870796485	8.628115529973009
>500	0.007769500300560352	2.4071928912907095
>1k	0.004258283818572996	3.720074130936478
>5k	4.482404019550521E-4	1.5328124198531339
>10k+	2.9882693463670145E-4	3.6553985014068098
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	37072	1.260924932246373	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	31255	1.0630720963897384	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	25861	0.8796067024391306	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13198	0.44890179261403834	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8901	0.3027485115970264	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7838	0.2665928360743168	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7785	0.26479015422793517	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7750	0.2635997039520228	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6861	0.23336226694384887	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5895	0.20050583932866767	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4248	0.14448665063073457	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3665	0.12465715032053724	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3542	0.12047356792233095	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3521	0.11975929775678353	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3442	0.11707228141972419	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3383	0.11506552238318621	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3245	0.1103717470095889	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3224	0.1096574768440415	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3116	0.10598408742122621	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	2991	0.10173247929296776	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.401286502606746E-5	0.0
5	3.401286502606746E-5	0.0	0.0	3.401286502606746E-5	0.0
6	3.401286502606746E-5	0.0	0.0	3.401286502606746E-5	0.0
7	6.802573005213492E-5	0.0	0.0	3.401286502606746E-5	0.0
8	1.0203859507820237E-4	0.0	0.0	3.401286502606746E-5	0.0
9	1.0203859507820237E-4	0.0	0.0	1.0203859507820237E-4	0.0
10	1.700643251303373E-4	0.0	0.0	1.3605146010426983E-4	0.0
11	2.7210292020853966E-4	0.0	0.0	1.3605146010426983E-4	0.0
12	2.7210292020853966E-4	0.0	0.0	2.0407719015640475E-4	0.0
13	3.401286502606746E-4	0.0	0.0	3.401286502606746E-4	0.0
14	3.741415152867421E-4	0.0	0.0	4.7618011036494444E-4	0.0
15	3.741415152867421E-4	0.0	0.0	6.802573005213492E-4	0.0
16	3.741415152867421E-4	0.0	0.0	0.0010884116808341586	0.0
17	3.741415152867421E-4	0.0	0.0	0.001632617521251238	0.0
18	3.741415152867421E-4	0.0	0.0	0.00214281049664225	0.0
19	3.741415152867421E-4	0.0	0.0	0.0029251063922418013	0.0
20	3.741415152867421E-4	0.0	0.0	0.005544096999248996	0.0
21	3.741415152867421E-4	0.0	0.0	0.011190232593576195	0.0
22	3.741415152867421E-4	0.0	0.0	0.01897917868454564	0.0
23	3.741415152867421E-4	0.0	0.0	0.024149134168507898	0.0
24	3.741415152867421E-4	0.0	0.0	0.03234623463979015	0.0
25	3.741415152867421E-4	0.0	0.0	0.038400524614430165	0.0
26	4.081543803128095E-4	0.0	0.0	0.047890113956702986	0.0
27	4.081543803128095E-4	0.0	0.0	0.08921574496337495	0.0
28	4.081543803128095E-4	0.0	0.0	0.18618642315269326	0.0
29	4.081543803128095E-4	0.0	0.0	0.3128503325097685	0.0
30	4.4216724533887696E-4	0.0	0.0	0.48189427168932375	0.0
31	4.4216724533887696E-4	0.0	0.0	0.7171952719396585	3.401286502606746E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9645	0.0	22.710213	1
TATACCG	135	5.638867E-11	19.185184	5
GTATTGG	1575	0.0	18.55873	1
ATTGGAC	1720	0.0	15.918606	3
TAATACT	730	0.0	15.458904	4
TTGGACC	2120	0.0	15.096699	4
TGGACCC	1985	0.0	15.005038	5
GTATCAA	15080	0.0	14.500664	2
TAGTACT	530	0.0	14.311321	4
TATTGGA	1965	0.0	14.310433	2
TATACTG	365	0.0	14.191781	5
TTATGCG	340	0.0	14.1470585	4
TGCGCTA	105	1.657016E-4	14.095238	5
GGACCCT	2225	0.0	13.968539	6
GTATTAG	625	0.0	13.912	1
GTTTCGC	80	0.0063016987	13.875001	32
TCTAATA	720	0.0	13.875001	2
CCGTTTA	360	0.0	13.875001	27
CTCGTCT	515	0.0	13.650486	13
CTCTAAT	615	0.0	13.536585	1
>>END_MODULE