FastQCFastQC Report
Wed 25 May 2016
SRR2088574_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088574_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976903
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT163391.6725304354680046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT146511.499739482835041No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT88160.9024437431351935No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60950.6239104598921285No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG33610.34404644063944934No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG32200.32961307315055843No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA30420.3113922262496891No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA25650.26256445112769644No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA25240.2583675144819905No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG20420.2090279178178386No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18350.18783850597244559No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15290.15651502759229935No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13680.14003437393477142No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC12270.1256010064458805No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA11360.11628585437858212No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC11270.11536457560269546No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG10710.10963217433051184No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC10030.10267140135714602No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT10010.10246667274028229No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA9850.1008288438053727No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA9810.1004193865716453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46550.023.3286781
AGCGTAA400.001930981123.12527
TAGACCA500.007034144418.54
GTTAGGC500.007034144418.511
CGTAGAC500.007034144418.53
GTATCAA62100.017.5169092
CGTTTAT852.7224381E-517.4117641
ATTAGAC650.001579704917.0769233
ATGCCGT650.001579704917.0769236
GCATATA650.001579704917.0769231
TTCGTCT803.381916E-416.187518
GTATTGG3600.015.9305561
ACTGTCG700.0025921215.8571428
GCCGTCA1059.34015E-615.85714213
CTAATTG700.0025921215.85714214
ATTAATC1557.201379E-915.5161293
ACTGAGC1201.9346771E-615.4166678
CGAACTA1701.4842954E-915.23529424
ATTTTCG855.363127E-415.23529415
GGGCGTC855.363127E-415.2352947
Produced by FastQC (version 0.11.5)