##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088574_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	976903
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.87405504947779	31.0	31.0	31.0	28.0	34.0
2	31.048427530676022	31.0	31.0	34.0	28.0	34.0
3	31.02867224279176	31.0	31.0	34.0	27.0	34.0
4	34.78869857089189	35.0	35.0	37.0	32.0	37.0
5	34.61661495563019	35.0	35.0	37.0	32.0	37.0
6	34.80808125269346	35.0	35.0	37.0	32.0	37.0
7	34.67241578744256	35.0	35.0	37.0	31.0	37.0
8	34.75923709928212	35.0	35.0	37.0	32.0	37.0
9	36.21563348664095	38.0	35.0	39.0	32.0	39.0
10	35.95836331754534	37.0	35.0	39.0	30.0	39.0
11	36.10441261824357	38.0	35.0	39.0	31.0	39.0
12	35.91787823356055	37.0	35.0	39.0	30.0	39.0
13	36.07140319970355	37.0	35.0	39.0	31.0	39.0
14	36.81115627651875	38.0	36.0	40.0	31.0	41.0
15	36.82731755353397	38.0	36.0	40.0	31.0	41.0
16	36.655378271947164	38.0	36.0	40.0	31.0	41.0
17	36.740135919328736	38.0	36.0	40.0	31.0	41.0
18	36.734600057528745	38.0	36.0	40.0	31.0	41.0
19	36.783001997127656	39.0	36.0	40.0	31.0	41.0
20	36.66032553897367	38.0	35.0	40.0	30.0	41.0
21	36.539073991993064	38.0	35.0	40.0	30.0	41.0
22	36.369713267335655	38.0	35.0	40.0	30.0	41.0
23	36.20136902026097	38.0	34.0	40.0	29.0	41.0
24	36.058442854613	38.0	34.0	40.0	29.0	41.0
25	35.833234210561336	38.0	34.0	40.0	27.0	41.0
26	35.89719757232806	38.0	34.0	40.0	29.0	41.0
27	35.771511603506184	38.0	34.0	40.0	28.0	41.0
28	35.61482358023263	38.0	34.0	40.0	27.0	41.0
29	35.424403446401534	38.0	34.0	40.0	27.0	41.0
30	35.28915255659979	38.0	34.0	40.0	27.0	41.0
31	35.02655841982264	37.0	33.0	40.0	26.0	41.0
32	34.87596312018696	37.0	33.0	40.0	25.0	41.0
33	34.63131139939175	37.0	33.0	40.0	25.0	41.0
34	34.435610290888654	37.0	33.0	40.0	25.0	41.0
35	34.188260246923186	37.0	33.0	40.0	24.0	41.0
36	34.01369634446818	37.0	33.0	40.0	23.0	41.0
37	33.81407161202289	37.0	33.0	40.0	22.0	41.0
38	33.667431669265014	37.0	32.0	40.0	21.0	41.0
39	33.43185863898463	37.0	32.0	40.0	20.0	41.0
40	33.202591250103644	37.0	31.0	40.0	18.0	41.0
41	33.00660659246619	36.0	31.0	40.0	18.0	41.0
42	32.72954428433529	36.0	31.0	39.0	15.0	41.0
43	32.3181277977445	36.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	4.0
14	6.0
15	24.0
16	44.0
17	95.0
18	233.0
19	439.0
20	892.0
21	1654.0
22	2833.0
23	4563.0
24	6948.0
25	10049.0
26	14130.0
27	18611.0
28	24339.0
29	30699.0
30	37712.0
31	44890.0
32	53068.0
33	63807.0
34	76480.0
35	93893.0
36	120541.0
37	151163.0
38	153679.0
39	66106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.739363068800074	25.38614376248205	16.154828063789342	19.719665104928534
2	22.061351024615544	25.951092380717434	31.67325722205787	20.314299372609153
3	23.651171098870616	25.24692830301473	31.961822207527256	19.140078390587398
4	17.48996573866597	18.874647738823608	33.249053386057774	30.38633313645265
5	12.260992135350184	40.92852616892363	33.73651222281025	13.07396947291594
6	31.816669618170895	38.022505816851826	13.12986038531973	17.030964179657552
7	24.24949048165478	34.23594768364925	21.90053669606911	19.614025138626864
8	27.367814409414244	35.57159718006803	18.802378537070723	18.258209873447008
9	23.914452100157334	14.3905792079664	22.5477862182837	39.147182473592565
10	16.823164633540895	28.829781462437932	32.9900716857252	21.356982218295983
11	33.00962326863568	22.530281921541853	23.582484647912842	20.877610161909626
12	19.718539097535785	28.39647334484591	31.927427799894154	19.957559757724155
13	32.33186918250839	20.673598095204948	27.314892061954975	19.679640660331682
14	19.518416874551516	23.24928882396717	29.402714496730997	27.82957980475032
15	22.72047480660823	29.941867309241555	26.476426011589687	20.86123187256053
16	20.60839203073386	28.28182531940223	28.484813743022592	22.624968906841314
17	21.292492703983918	26.882095765905113	28.792213761243442	23.033197768867534
18	22.21581876603921	25.0466013514136	30.983424147535633	21.75415573501156
19	22.232504148313602	24.428320928485224	32.73098762108418	20.608187302116995
20	22.836863025295244	24.210899137375975	32.624016918772895	20.328220918555886
21	22.000034803864867	24.936252626924066	33.26891206189356	19.79480050731751
22	20.44737297357056	25.307323244989522	32.867746337149136	21.377557444290783
23	21.362714619568166	25.66959053252984	33.72699234212609	19.240702505775907
24	21.288295767338212	25.64799166345072	31.380802392868073	21.682910176342997
25	21.528954256461493	25.986203338509554	31.814110510460097	20.670731894568856
26	21.278571158037185	26.552892149988278	32.119565606820736	20.048971085153795
27	20.46242052691004	26.35880942120149	31.988232199102672	21.19053785278579
28	20.608084937808567	26.629870109929033	32.07534422557818	20.686700726684226
29	20.81025444696147	25.66846452513709	31.60805115758678	21.91322987031466
30	20.934422353089303	26.194719434785235	32.89139249239689	19.97946571972857
31	21.434574364087325	25.982415859097575	32.62749730525958	19.95551247155552
32	21.103118733384992	25.822625173635462	31.852804219047336	21.221451873932214
33	20.852530906343823	25.975147993198917	32.14300703345163	21.02931406700563
34	20.334362777061795	26.57080590396385	32.457879646188005	20.636951672786346
35	19.796028879018696	28.180177561129405	31.67991090210594	20.343882657745958
36	22.07865059274053	25.95283257396077	31.399432697002673	20.569084136296027
37	21.153072515899737	26.695178538708554	31.151608706289164	21.000140239102553
38	21.140174613037324	25.05253848130265	32.42645380349943	21.380833102160604
39	20.196170960678796	25.199635992519216	33.21711572182704	21.387077324974946
40	19.496203819621805	26.10801686554346	32.26594656787828	22.129832746956453
41	20.744638925256652	25.164729763343956	32.30115988997884	21.78947142142055
42	19.876794318371424	27.016397738567697	31.21517694182534	21.891631001235538
43	20.127996331263187	27.00994878713649	30.585329352044166	22.276725529556156
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	72.0
1	113.5
2	155.0
3	341.5
4	528.0
5	528.0
6	1094.5
7	1661.0
8	2007.0
9	2353.0
10	3593.0
11	4833.0
12	4833.0
13	9735.5
14	14638.0
15	23580.5
16	32523.0
17	28668.0
18	24813.0
19	24813.0
20	27900.5
21	30988.0
22	24286.5
23	17585.0
24	18501.0
25	19417.0
26	19417.0
27	20591.0
28	21765.0
29	23582.5
30	25400.0
31	27375.5
32	29351.0
33	29351.0
34	32079.0
35	34807.0
36	38034.5
37	41262.0
38	43610.5
39	45959.0
40	45959.0
41	48063.5
42	50168.0
43	53436.0
44	56704.0
45	61511.0
46	66318.0
47	66318.0
48	85934.5
49	105551.0
50	97189.0
51	88827.0
52	81168.5
53	73510.0
54	73510.0
55	63804.0
56	54098.0
57	45879.0
58	37660.0
59	33104.0
60	28548.0
61	28548.0
62	24850.5
63	21153.0
64	18320.0
65	15487.0
66	13103.5
67	10720.0
68	10720.0
69	9134.0
70	7548.0
71	6249.5
72	4951.0
73	4109.5
74	3268.0
75	3268.0
76	2620.0
77	1972.0
78	1503.0
79	1034.0
80	808.0
81	582.0
82	582.0
83	460.5
84	339.0
85	248.0
86	157.0
87	129.5
88	102.0
89	102.0
90	79.0
91	56.0
92	42.5
93	29.0
94	18.5
95	8.0
96	8.0
97	5.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	976903.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.60573690736486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.39854552821889	47.486490549137585
2	8.223944364437251	9.145969733394072
3	2.337401910913816	3.8991886711513666
4	1.0949156224039374	2.435343601406279
5	0.5941437555119755	1.6518900677076307
6	0.40171682152522314	1.340265593339664
7	0.2712289012714172	1.0557318048040443
8	0.2133437560832098	0.9490509417273588
9	0.16788672510105107	0.8401918559587319
>10	1.126623991590012	11.994691963398736
>50	0.10882905981097726	4.192003752556047
>100	0.05238184972276903	5.263428606705145
>500	0.005533293924153454	2.1611645925114447
>1k	0.002766646962076727	2.876937546722595
>5k	3.688862616102303E-4	1.5292865052647184
>10k+	3.688862616102303E-4	3.178364214214582
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	16339	1.6725304354680046	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14651	1.499739482835041	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8816	0.9024437431351935	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6095	0.6239104598921285	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3361	0.34404644063944934	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3220	0.32961307315055843	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3042	0.3113922262496891	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2565	0.26256445112769644	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2524	0.2583675144819905	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2042	0.2090279178178386	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1835	0.18783850597244559	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1529	0.15651502759229935	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1368	0.14003437393477142	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1227	0.1256010064458805	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1136	0.11628585437858212	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1127	0.11536457560269546	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1071	0.10963217433051184	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	1003	0.10267140135714602	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	1001	0.10246667274028229	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	985	0.1008288438053727	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	981	0.1004193865716453	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.0709292529555135E-4	0.0
10	2.047286168637009E-4	0.0	0.0	5.118215421592523E-4	0.0
11	2.047286168637009E-4	0.0	0.0	7.165501590229532E-4	0.0
12	2.047286168637009E-4	0.0	0.0	0.0010236430843185045	0.0
13	2.047286168637009E-4	0.0	0.0	0.0010236430843185045	0.0
14	3.0709292529555135E-4	0.0	0.0	0.0010236430843185045	0.0
15	3.0709292529555135E-4	0.0	0.0	0.0015354646264777567	0.0
16	3.0709292529555135E-4	0.0	0.0	0.002456743402364411	0.0
17	3.0709292529555135E-4	0.0	0.0	0.003787479411978467	0.0
18	3.0709292529555135E-4	0.0	0.0	0.004196936645705868	0.0
19	3.0709292529555135E-4	0.0	0.0	0.005425308346888074	0.0
20	3.0709292529555135E-4	0.0	0.0	0.010748252385344298	0.0
21	3.0709292529555135E-4	0.0	0.0	0.019039761368324182	0.0
22	3.0709292529555135E-4	0.0	0.0	0.032551850081328444	0.0
23	3.0709292529555135E-4	0.0	0.0	0.042788280924513485	0.0
24	3.0709292529555135E-4	0.0	0.0	0.0566074625628133	0.0
25	3.0709292529555135E-4	0.0	0.0	0.06745807925658945	0.0
26	3.0709292529555135E-4	0.0	0.0	0.0791276104178204	0.0
27	3.0709292529555135E-4	0.0	0.0	0.1249868205952894	0.0
28	3.0709292529555135E-4	0.0	0.0	0.23083151551382278	0.0
29	3.0709292529555135E-4	0.0	0.0	0.3652358524848424	0.0
30	3.0709292529555135E-4	0.0	0.0	0.5598304028137902	0.0
31	3.0709292529555135E-4	0.0	0.0	0.8290485339895568	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4655	0.0	23.328678	1
AGCGTAA	40	0.0019309811	23.125	27
TAGACCA	50	0.0070341444	18.5	4
GTTAGGC	50	0.0070341444	18.5	11
CGTAGAC	50	0.0070341444	18.5	3
GTATCAA	6210	0.0	17.516909	2
CGTTTAT	85	2.7224381E-5	17.411764	1
ATTAGAC	65	0.0015797049	17.076923	3
ATGCCGT	65	0.0015797049	17.076923	6
GCATATA	65	0.0015797049	17.076923	1
TTCGTCT	80	3.381916E-4	16.1875	18
GTATTGG	360	0.0	15.930556	1
ACTGTCG	70	0.00259212	15.857142	8
GCCGTCA	105	9.34015E-6	15.857142	13
CTAATTG	70	0.00259212	15.857142	14
ATTAATC	155	7.201379E-9	15.516129	3
ACTGAGC	120	1.9346771E-6	15.416667	8
CGAACTA	170	1.4842954E-9	15.235294	24
ATTTTCG	85	5.363127E-4	15.235294	15
GGGCGTC	85	5.363127E-4	15.235294	7
>>END_MODULE