FastQCFastQC Report
Wed 25 May 2016
SRR2088573_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088573_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1039921
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT200601.9289926830980428No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT184281.7720576851510836No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT97000.9327631618170996No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71870.6911101901009787No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG34480.3315636476232329No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA34110.32800568504722954No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG28170.27088596152977007No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21410.20588102365468147No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA20940.20136144957165017No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA20690.1989574208040803No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19880.19116836759715403No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG18490.17780196764946568No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15830.15222310156252256No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT15340.14751120517808566No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC12490.12010527722778942No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC12160.11693195925459722No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC11640.11193157941805194No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT11460.11020067870540165No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA11140.10712352188291226No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA10960.10539262117026198No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10450.1004884024844195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48800.025.8165971
GCTATCC705.101152E-621.1428593
TATCTCG500.007034441518.510
TCGTAGC500.007034441518.52
CTAGGCG500.007034441518.522
GTATCAA69050.018.2454742
CTATGCT650.001579797417.0769234
TTCGTTA1302.5880763E-715.65384630
GTATTAG1557.203198E-915.5161291
GCGTTTC855.3635397E-415.23529433
TAGATGT1701.4842954E-915.2352944
ACGTTCG1101.4515244E-515.13636422
ATACACA4300.015.05813937
CGAACTA1001.09320434E-414.824
CTTCGTT1508.097231E-814.79999929
TACGCTG750.004104057314.7999995
TACACTG1252.957906E-614.7999995
CGCCACG1252.957906E-614.79999918
TACCCTT1152.2093327E-514.4782615
CGTTCGC1304.4426924E-614.23076923
Produced by FastQC (version 0.11.5)