##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088573_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1039921
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.65086674853186	31.0	30.0	31.0	28.0	34.0
2	30.81323773632805	31.0	31.0	33.0	27.0	34.0
3	30.69778185073674	31.0	30.0	34.0	27.0	34.0
4	34.47020302503748	35.0	35.0	37.0	30.0	37.0
5	34.20920050657694	35.0	35.0	37.0	30.0	37.0
6	34.520465496898325	35.0	35.0	37.0	31.0	37.0
7	34.39703977513677	35.0	35.0	37.0	30.0	37.0
8	34.50675676325413	35.0	35.0	37.0	30.0	37.0
9	35.928661888739626	37.0	35.0	39.0	30.0	39.0
10	35.59688572497334	37.0	35.0	39.0	30.0	39.0
11	35.81507345269497	37.0	35.0	39.0	30.0	39.0
12	35.57443690434177	37.0	35.0	39.0	30.0	39.0
13	35.772124997956574	37.0	35.0	39.0	30.0	39.0
14	36.40474516814258	38.0	35.0	40.0	30.0	41.0
15	36.45237186286266	38.0	35.0	40.0	30.0	41.0
16	36.240079775290624	38.0	35.0	40.0	30.0	41.0
17	36.3533278008618	38.0	35.0	40.0	30.0	41.0
18	36.35768293937713	38.0	35.0	40.0	30.0	41.0
19	36.42594100898049	38.0	35.0	40.0	30.0	41.0
20	36.29666676603319	38.0	35.0	40.0	30.0	41.0
21	36.18564871754681	38.0	34.0	40.0	29.0	41.0
22	35.99863547327153	38.0	34.0	40.0	29.0	41.0
23	35.81023750842612	38.0	34.0	40.0	27.0	41.0
24	35.66990761798252	38.0	34.0	40.0	27.0	41.0
25	35.44691567917178	38.0	34.0	40.0	27.0	41.0
26	35.46480357642552	38.0	34.0	40.0	27.0	41.0
27	35.32300434359918	38.0	34.0	40.0	27.0	41.0
28	35.15713308991741	38.0	33.0	40.0	27.0	41.0
29	34.95436480271098	37.0	33.0	40.0	26.0	41.0
30	34.813884900872274	37.0	33.0	40.0	25.0	41.0
31	34.53488678466922	37.0	33.0	39.0	25.0	41.0
32	34.37812680001654	37.0	33.0	39.0	25.0	40.0
33	34.1011288357481	36.0	33.0	39.0	24.0	40.0
34	33.90883057463019	36.0	33.0	39.0	24.0	40.0
35	33.65079751250335	36.0	32.0	39.0	23.0	40.0
36	33.49786570326015	36.0	32.0	39.0	22.0	40.0
37	33.27223510247413	36.0	31.0	39.0	21.0	40.0
38	33.101725034882456	36.0	31.0	39.0	20.0	40.0
39	32.82655605570039	36.0	31.0	39.0	18.0	40.0
40	32.57551871728718	36.0	30.0	39.0	16.0	40.0
41	32.375436211019874	35.0	30.0	39.0	15.0	40.0
42	32.0763356062624	35.0	30.0	39.0	15.0	40.0
43	31.645886562536962	35.0	29.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	1.0
13	2.0
14	10.0
15	33.0
16	71.0
17	167.0
18	331.0
19	670.0
20	1231.0
21	2331.0
22	3777.0
23	5861.0
24	8745.0
25	12633.0
26	17086.0
27	23027.0
28	29405.0
29	36556.0
30	44198.0
31	52573.0
32	62769.0
33	74881.0
34	89313.0
35	107854.0
36	134423.0
37	152490.0
38	128121.0
39	51358.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.96363281441571	25.783112371035877	16.159977536755196	19.093277277793216
2	24.33790643712359	26.22199186284343	29.318957882377607	20.12114381765538
3	25.2092226236416	24.260881355410653	31.82943704377544	18.700458977172303
4	19.81967861020212	19.43753419730922	31.157751406116425	29.58503578637223
5	12.220543675913843	41.63585503129565	33.2323320713785	12.911269221412011
6	32.216774158806295	38.053659845315174	12.572012681732556	17.15755331414598
7	24.36319681975842	34.68571170310052	21.2679617009369	19.683129776204154
8	28.21541251691234	35.72482909759491	18.066756994040894	17.993001391451852
9	24.457434747447163	13.803933183386047	21.74338243001151	39.99524963915528
10	17.364203627006283	29.472238756597857	32.02002844446838	21.143529171927483
11	33.96459923398027	22.18283888872328	22.548251261393894	21.304310615902555
12	19.5952384844618	29.1536568643195	31.469602017845588	19.781502633373112
13	33.12703561135894	20.51184657296083	26.744339233461	19.616778582219226
14	19.523117621434704	23.438607355751063	29.349921772903905	27.68835324991033
15	23.294461790847574	30.120461073485387	26.038997193056012	20.546079942611026
16	20.696379821159493	27.93606437412073	28.56091953138748	22.806636273332302
17	21.297867818805464	27.1330226046017	28.990086746974047	22.57902282961879
18	21.986093174385363	24.75659208728355	31.01360584121294	22.243708897118147
19	22.44064693375747	24.302999939418473	33.03799038580815	20.218362741015905
20	22.887026995319836	24.003169471527166	32.840571543415315	20.269231989737683
21	22.010998912417385	24.498976364550767	33.61255326125735	19.8774714617745
22	20.513385151372077	25.01613103303039	33.26887330864556	21.20161050695197
23	21.31315744176721	25.225570019261077	34.1409587843692	19.320313754602513
24	21.230555013313513	25.328943256266584	31.983775690653427	21.456726039766483
25	21.463938126069195	25.542805655429596	32.504584482859755	20.488671735641457
26	21.013711618478712	26.323153393382764	32.5164123043962	20.146722683742325
27	20.588102365468146	25.892447599384955	32.41698167456951	21.10246836057739
28	20.532136575759118	26.432103977129035	32.574685961722096	20.461073485389754
29	20.748883809443218	25.255091492526837	32.20513865957126	21.79088603845869
30	20.941109949698102	25.99755173710311	33.234928422447474	19.826409890751318
31	21.100256654111227	25.85071365997994	33.01779654416057	20.031233141748267
32	21.177666380426974	25.55530660502096	32.22985207530187	21.037174939250193
33	20.609065496321357	26.0420743498785	32.5094887015456	20.839371452254547
34	20.33365996070855	26.083904450434215	32.96221539905435	20.62022018980288
35	19.805062115295296	27.66210125576847	32.37611318552082	20.156723443415416
36	22.107352385421585	25.445394409767662	31.85876619473979	20.588487010070956
37	21.09140982824657	26.47835749061708	31.582399047619962	20.84783363351639
38	21.20738017599414	24.884774901170378	32.754795796988425	21.15304912584706
39	20.209804398603357	24.998052736698266	33.51014163575887	21.282001228939507
40	19.590045782323852	25.684547191565514	32.5062192224217	22.219187803688932
41	20.695514370803167	24.845444990532936	32.736140533752085	21.722900104911815
42	20.209708237452652	26.403544115370302	31.425464049673003	21.961283597504043
43	20.37173977638686	26.30401732439291	30.907540091987755	22.416702807232472
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	94.0
1	150.0
2	206.0
3	343.5
4	481.0
5	481.0
6	1066.5
7	1652.0
8	1939.0
9	2226.0
10	3748.5
11	5271.0
12	5271.0
13	10987.5
14	16704.0
15	27647.0
16	38590.0
17	32839.0
18	27088.0
19	27088.0
20	31507.0
21	35926.0
22	27114.0
23	18302.0
24	19111.0
25	19920.0
26	19920.0
27	20908.5
28	21897.0
29	23344.5
30	24792.0
31	27013.5
32	29235.0
33	29235.0
34	32433.5
35	35632.0
36	39255.5
37	42879.0
38	45306.0
39	47733.0
40	47733.0
41	50071.0
42	52409.0
43	55816.5
44	59224.0
45	63746.5
46	68269.0
47	68269.0
48	87409.5
49	106550.0
50	99192.5
51	91835.0
52	84245.5
53	76656.0
54	76656.0
55	67400.0
56	58144.0
57	50692.0
58	43240.0
59	38269.5
60	33299.0
61	33299.0
62	29362.0
63	25425.0
64	21795.5
65	18166.0
66	15639.0
67	13112.0
68	13112.0
69	11196.5
70	9281.0
71	7763.5
72	6246.0
73	5212.5
74	4179.0
75	4179.0
76	3241.5
77	2304.0
78	1831.5
79	1359.0
80	1055.0
81	751.0
82	751.0
83	577.0
84	403.0
85	321.0
86	239.0
87	174.0
88	109.0
89	109.0
90	83.0
91	57.0
92	38.0
93	19.0
94	16.0
95	13.0
96	13.0
97	8.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1039921.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.884153484201924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.05758671986608	46.68313644528945
2	8.343444390585255	9.158457650395695
3	2.4041844736002975	3.958548889602418
4	1.0762283383425835	2.3627152522256787
5	0.6494505618903811	1.7822272159596426
6	0.4220787179789841	1.3899259883984245
7	0.30217150130124654	1.1609098939178535
8	0.22701438926154113	0.9967594066682227
9	0.18554593998451077	0.9165178663632367
>10	1.162263486157501	12.066123903969116
>50	0.107485558751718	4.0705922884533186
>100	0.05444138041666709	5.448160261535791
>500	0.004404642317052125	1.6571140713707486
>1k	0.002995156775595445	2.9941576767184377
>5k	3.5237138536417E-4	1.6329395648735388
>10k+	3.5237138536417E-4	3.7217136242584687
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	20060	1.9289926830980428	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18428	1.7720576851510836	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9700	0.9327631618170996	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7187	0.6911101901009787	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3448	0.3315636476232329	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3411	0.32800568504722954	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2817	0.27088596152977007	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2141	0.20588102365468147	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2094	0.20136144957165017	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2069	0.1989574208040803	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1988	0.19116836759715403	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1849	0.17780196764946568	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1583	0.15222310156252256	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	1534	0.14751120517808566	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1249	0.12010527722778942	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1216	0.11693195925459722	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	1164	0.11193157941805194	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	1146	0.11020067870540165	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1114	0.10712352188291226	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1096	0.10539262117026198	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1045	0.1004884024844195	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	9.616115070279377E-5	0.0
5	0.0	0.0	0.0	9.616115070279377E-5	0.0
6	0.0	0.0	0.0	9.616115070279377E-5	0.0
7	0.0	0.0	0.0	9.616115070279377E-5	0.0
8	0.0	0.0	0.0	9.616115070279377E-5	0.0
9	0.0	0.0	0.0	9.616115070279377E-5	0.0
10	0.0	0.0	0.0	1.9232230140558753E-4	0.0
11	0.0	0.0	0.0	1.9232230140558753E-4	0.0
12	0.0	0.0	0.0	1.9232230140558753E-4	0.0
13	0.0	0.0	0.0	1.9232230140558753E-4	0.0
14	0.0	0.0	0.0	4.8080575351396883E-4	0.0
15	0.0	0.0	0.0	5.769669042167626E-4	0.0
16	9.616115070279377E-5	0.0	0.0	9.616115070279377E-4	0.0
17	9.616115070279377E-5	0.0	0.0	0.001250094959136319	0.0
18	9.616115070279377E-5	0.0	0.0	0.001634739561947494	0.0
19	1.9232230140558753E-4	0.0	0.0	0.002596351068975432	0.0
20	1.9232230140558753E-4	0.0	0.0	0.005000379836545276	0.0
21	1.9232230140558753E-4	0.0	0.0	0.010096920823793347	0.0
22	1.9232230140558753E-4	0.0	0.0	0.018174457482828022	0.0
23	1.9232230140558753E-4	0.0	0.0	0.02221322581234536	0.0
24	1.9232230140558753E-4	0.0	0.0	0.029232989813649305	0.0
25	1.9232230140558753E-4	0.0	0.0	0.03259863008824709	0.0
26	1.9232230140558753E-4	0.0	0.0	0.03894526603463148	0.0
27	1.9232230140558753E-4	0.0	0.0	0.07654427595942384	0.0
28	1.9232230140558753E-4	0.0	0.0	0.17049372019605336	0.0
29	1.9232230140558753E-4	0.0	0.0	0.2826176219155109	0.0
30	1.9232230140558753E-4	0.0	0.0	0.44474532200042116	0.0
31	3.8464460281117507E-4	0.0	0.0	0.656972981601487	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4880	0.0	25.816597	1
GCTATCC	70	5.101152E-6	21.142859	3
TATCTCG	50	0.0070344415	18.5	10
TCGTAGC	50	0.0070344415	18.5	2
CTAGGCG	50	0.0070344415	18.5	22
GTATCAA	6905	0.0	18.245474	2
CTATGCT	65	0.0015797974	17.076923	4
TTCGTTA	130	2.5880763E-7	15.653846	30
GTATTAG	155	7.203198E-9	15.516129	1
GCGTTTC	85	5.3635397E-4	15.235294	33
TAGATGT	170	1.4842954E-9	15.235294	4
ACGTTCG	110	1.4515244E-5	15.136364	22
ATACACA	430	0.0	15.058139	37
CGAACTA	100	1.09320434E-4	14.8	24
CTTCGTT	150	8.097231E-8	14.799999	29
TACGCTG	75	0.0041040573	14.799999	5
TACACTG	125	2.957906E-6	14.799999	5
CGCCACG	125	2.957906E-6	14.799999	18
TACCCTT	115	2.2093327E-5	14.478261	5
CGTTCGC	130	4.4426924E-6	14.230769	23
>>END_MODULE