FastQCFastQC Report
Wed 25 May 2016
SRR2088572_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088572_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146262
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT363721.6946672866593175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT285211.3288685165184866No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT212910.992003772139655No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117950.5495601189416763No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG64670.3013145645778567No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG63990.2981462654606008No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA60290.2809069908520022No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA58720.27359194730186714No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA54260.2528116325033943No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG45260.21087826183383016No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT34760.16195599605267205No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA29440.13716871472355194No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28950.13488567565376455No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA28190.13134463546389025No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC27710.12910818902818016No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27380.12757063210362948No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT23540.1096790606179488No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA23490.10944609744756233No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA23160.10790854052301165No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC22940.10688350257331118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97300.021.1428591
TAGGAGT1502.5102054E-1017.2666664
ACGTTTA1757.2759576E-1216.91428626
TCACGTT1801.0913936E-1116.44444524
GTATTGG10850.016.0276491
CATTGCG1403.477362E-815.85714329
CTAATAC4850.015.2577323
CGAACTA3550.015.11267724
TAGACTG2006.184564E-1114.7999995
ATAGTAC3150.014.6825393
ATTGGAC12100.014.5247933
GTATCAA142850.014.414072
TAATACT4650.014.322584
CTGTGCG2201.8189894E-1114.2954549
CCGTTTA2900.014.03448327
TCTATAC1458.91956E-714.0344833
AGTACCG2151.9826984E-1013.7674425
TAGGTCG1356.572849E-613.70370421
GAGTAAT4200.013.6547631
TAATACA4750.013.6315784
Produced by FastQC (version 0.11.5)