##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088572_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2146262
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.248121617957175	31.0	31.0	33.0	30.0	34.0
2	31.430844882870776	31.0	31.0	34.0	30.0	34.0
3	31.4800112940545	31.0	31.0	34.0	28.0	34.0
4	35.220368249542695	37.0	35.0	37.0	33.0	37.0
5	35.11297781911062	37.0	35.0	37.0	32.0	37.0
6	35.19633017776954	37.0	35.0	37.0	32.0	37.0
7	35.044340812072335	37.0	35.0	37.0	32.0	37.0
8	35.094704653951844	37.0	35.0	37.0	32.0	37.0
9	36.60847790251144	38.0	35.0	39.0	32.0	39.0
10	36.412728734888844	38.0	35.0	39.0	32.0	39.0
11	36.51228880723789	38.0	35.0	39.0	32.0	39.0
12	36.363243164161695	38.0	35.0	39.0	32.0	39.0
13	36.45383601815622	38.0	35.0	39.0	32.0	39.0
14	37.295418266735375	39.0	36.0	40.0	32.0	41.0
15	37.28332002337086	39.0	36.0	40.0	32.0	41.0
16	37.16654397273026	39.0	36.0	40.0	31.0	41.0
17	37.23493264102891	39.0	36.0	40.0	32.0	41.0
18	37.239699067495025	39.0	36.0	40.0	32.0	41.0
19	37.28628424675086	39.0	36.0	40.0	31.0	41.0
20	37.16441003008952	39.0	36.0	40.0	31.0	41.0
21	37.0671437131161	39.0	36.0	40.0	31.0	41.0
22	36.90647320783763	39.0	36.0	40.0	31.0	41.0
23	36.75733391356694	39.0	35.0	40.0	30.0	41.0
24	36.61262697657602	38.0	35.0	40.0	30.0	41.0
25	36.40724245222624	38.0	35.0	40.0	30.0	41.0
26	36.520605126494345	38.0	35.0	40.0	30.0	41.0
27	36.40891978705302	38.0	35.0	40.0	30.0	41.0
28	36.27109225248362	38.0	35.0	40.0	30.0	41.0
29	36.09118085303658	38.0	35.0	40.0	29.0	41.0
30	35.952985236657966	38.0	35.0	40.0	29.0	41.0
31	35.72499536403291	38.0	34.0	40.0	28.0	41.0
32	35.59419912387211	38.0	34.0	40.0	27.0	41.0
33	35.37047480689683	38.0	34.0	40.0	27.0	41.0
34	35.20524940571095	38.0	34.0	40.0	26.0	41.0
35	34.99334657185376	38.0	33.0	40.0	25.0	41.0
36	34.833339545684545	38.0	33.0	40.0	25.0	41.0
37	34.64915513576628	38.0	33.0	40.0	24.0	41.0
38	34.51861422324022	38.0	33.0	40.0	24.0	41.0
39	34.33970037208878	38.0	33.0	40.0	23.0	41.0
40	34.11123525459613	37.0	33.0	40.0	22.0	41.0
41	33.940434578816564	37.0	33.0	40.0	21.0	41.0
42	33.67129455770078	37.0	33.0	40.0	19.0	41.0
43	33.2726130360599	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	5.0
14	8.0
15	18.0
16	56.0
17	134.0
18	274.0
19	658.0
20	1301.0
21	2498.0
22	4433.0
23	7352.0
24	11616.0
25	17260.0
26	24508.0
27	33477.0
28	44659.0
29	57062.0
30	70109.0
31	84495.0
32	102108.0
33	123505.0
34	149491.0
35	181299.0
36	234795.0
37	321085.0
38	401831.0
39	272222.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.97019003271735	23.1215480682228	15.464421398692238	21.443840500367617
2	19.874880140448838	25.074058991865854	33.67231027712367	21.378750590561637
3	21.218704892506135	25.102713461823395	32.979431215760236	20.699150429910233
4	14.78854864876702	17.37830702868522	35.36548659949251	32.46765772305525
5	12.329016681094853	39.85533918971682	34.35591740430572	13.459726724882609
6	31.93361295126131	37.96325891247201	13.143455924765943	16.959672211500738
7	25.007384932501253	33.81642129432474	21.442629091881606	19.733564681292407
8	27.414779742640928	34.84872769494125	18.747711136850953	18.98878142556687
9	24.223696827321177	14.309483185184288	22.14683016332582	39.319989824168715
10	16.532138201207495	28.36620133049926	33.142179286592224	21.959481181701022
11	33.087199978381015	22.574923285227992	23.415780552420905	20.922096183970083
12	20.516833452765784	28.049883937748515	31.180070280329243	20.253212329156458
13	32.054846985130425	20.856866496261873	26.721062013864106	20.367224504743596
14	19.90842683698449	23.26165211889322	28.73251261961494	28.097408424507353
15	22.53326947036289	30.03962237601933	26.04155503848086	21.385553115136922
16	20.957366807966597	28.492280998312413	27.46789534548904	23.08245684823195
17	21.36756835838309	26.83628559793725	27.978923356048796	23.817222687630867
18	22.43621701357989	25.25423270784275	30.65786003759094	21.651690240986422
19	22.347131897224102	24.685103682588615	31.96012416005129	21.007640260135997
20	23.162922327283436	24.332211072087194	31.743421818957795	20.761444781671575
21	22.11668472907781	25.26825709070002	32.29265578946093	20.32240239076124
22	20.707956437750845	25.701335624448458	31.85943747781026	21.73127045999044
23	21.62885985028855	25.960111114113747	32.65803522589506	19.752993809702637
24	21.661101953070037	25.92064715305028	30.353749914968443	22.06450097891124
25	21.624573327953435	26.10930072842924	31.13426972103126	21.13185622258606
26	21.276013832421206	26.94307591524241	31.30568402180163	20.475226230534762
27	20.408691949072388	26.912417962019546	30.93233724494027	21.74655284396779
28	20.718719336222698	26.75898841800302	31.147874770181833	21.37441747559245
29	21.0357356184846	26.34752886646644	30.588064271743153	22.0286712433058
30	20.91804262480536	26.63887260735176	32.20594689744309	20.237137870399792
31	21.554730969471574	26.378186819689304	31.95094541113806	20.116136799701064
32	21.185158195970484	26.144990686132445	30.860351625290853	21.809499492606214
33	20.808596527357796	26.308763794914135	31.48422699558581	21.398412682142254
34	20.23327068177138	27.00961019670478	31.84918709831325	20.907932023210588
35	19.73659320250743	28.857707027380624	30.833001749087487	20.57269802102446
36	21.91009298957909	26.511069012077744	30.746525820240027	20.83231217810314
37	20.900710164928608	27.257483009995987	30.703101485279987	21.138705339795422
38	20.835666847756702	25.517900424086154	32.09631442945921	21.550118298697925
39	20.208157252003716	25.576141216682775	32.79389934686446	21.421802184449056
40	19.248069434207007	26.358338357572375	32.08862664483647	22.304965563384155
41	20.671520997902398	25.259823823932027	32.15767692853901	21.91097824962656
42	19.572773501091664	27.192066951751464	31.426824870402587	21.80833467675428
43	19.87856095854094	27.10573080080624	30.617604001748155	22.39810423890466
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	174.0
1	277.0
2	380.0
3	906.0
4	1432.0
5	1432.0
6	2593.0
7	3754.0
8	4389.0
9	5024.0
10	7670.5
11	10317.0
12	10317.0
13	19980.0
14	29643.0
15	49581.0
16	69519.0
17	61300.0
18	53081.0
19	53081.0
20	57392.0
21	61703.0
22	48432.0
23	35161.0
24	38018.5
25	40876.0
26	40876.0
27	43778.5
28	46681.0
29	50233.0
30	53785.0
31	58920.5
32	64056.0
33	64056.0
34	70538.0
35	77020.0
36	84149.0
37	91278.0
38	96847.5
39	102417.0
40	102417.0
41	107042.0
42	111667.0
43	118557.0
44	125447.0
45	133976.5
46	142506.0
47	142506.0
48	185640.0
49	228774.0
50	213368.5
51	197963.0
52	181026.0
53	164089.0
54	164089.0
55	144197.5
56	124306.0
57	104973.0
58	85640.0
59	74939.5
60	64239.0
61	64239.0
62	56293.5
63	48348.0
64	41570.0
65	34792.0
66	29872.0
67	24952.0
68	24952.0
69	21371.5
70	17791.0
71	14770.0
72	11749.0
73	9622.5
74	7496.0
75	7496.0
76	6019.0
77	4542.0
78	3532.5
79	2523.0
80	2007.5
81	1492.0
82	1492.0
83	1139.0
84	786.0
85	594.5
86	403.0
87	312.5
88	222.0
89	222.0
90	189.5
91	157.0
92	104.0
93	51.0
94	36.0
95	21.0
96	21.0
97	11.5
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2146262.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.061949449006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.0463946412525	38.95247722272068
2	10.701625112621045	10.286819303700117
3	3.269773924432257	4.7145512719722324
4	1.4379544813881955	2.7644358237780433
5	0.7722002108493847	1.855672374917745
6	0.48484742310156503	1.3981627403752945
7	0.33389029099322665	1.1233192801061251
8	0.23448403444204619	0.9015807847962083
9	0.19187906816812222	0.8299873867156798
>10	1.2783557946882256	11.941911210524053
>50	0.1395874964203198	4.6460011590596775
>100	0.09688627852423073	8.621272315808438
>500	0.007078805981502094	2.308890304701033
>1k	0.0041697076329395896	3.681379971505109
>5k	4.8484972476041735E-4	1.4071642673712337
>10k+	3.8787977980833384E-4	4.566374581948336
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	36372	1.6946672866593175	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28521	1.3288685165184866	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21291	0.992003772139655	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11795	0.5495601189416763	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6467	0.3013145645778567	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6399	0.2981462654606008	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6029	0.2809069908520022	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5872	0.27359194730186714	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5426	0.2528116325033943	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4526	0.21087826183383016	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3476	0.16195599605267205	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2944	0.13716871472355194	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2895	0.13488567565376455	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2819	0.13134463546389025	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2771	0.12910818902818016	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2738	0.12757063210362948	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2354	0.1096790606179488	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2349	0.10944609744756233	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2316	0.10790854052301165	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	2294	0.10688350257331118	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	4.6592634077293455E-5	0.0	0.0	0.0	0.0
6	9.318526815458691E-5	0.0	0.0	0.0	0.0
7	9.318526815458691E-5	0.0	0.0	0.0	0.0
8	1.3977790223188035E-4	0.0	0.0	0.0	0.0
9	1.3977790223188035E-4	0.0	0.0	9.318526815458691E-5	0.0
10	1.3977790223188035E-4	0.0	0.0	9.318526815458691E-5	0.0
11	1.3977790223188035E-4	0.0	0.0	2.3296317038646726E-4	0.0
12	1.3977790223188035E-4	0.0	0.0	5.12518974850228E-4	0.0
13	1.8637053630917382E-4	0.0	0.0	6.057042430048149E-4	0.0
14	2.795558044637607E-4	0.0	0.0	6.988895111594018E-4	0.0
15	2.795558044637607E-4	0.0	0.0	8.852600474685756E-4	0.0
16	3.261484385410542E-4	0.0	0.0	0.0014909642904733906	0.0
17	3.261484385410542E-4	0.0	0.0	0.002376224337941966	0.0
18	3.261484385410542E-4	0.0	0.0	0.002795558044637607	0.0
19	3.261484385410542E-4	0.0	0.0	0.004100151798801824	0.0
20	3.261484385410542E-4	0.0	0.0	0.007175265647903191	0.0
21	4.1933370669564106E-4	0.0	0.0	0.01309253017571946	0.0
22	4.1933370669564106E-4	0.0	0.0	0.021525796943709576	0.0
23	4.1933370669564106E-4	4.6592634077293455E-5	0.0	0.027443061471525844	0.0
24	4.1933370669564106E-4	4.6592634077293455E-5	0.0	0.03564336506912949	0.0
25	4.1933370669564106E-4	4.6592634077293455E-5	0.0	0.04211974120587328	0.0
26	4.1933370669564106E-4	4.6592634077293455E-5	0.0	0.05171782382579573	0.0
27	4.1933370669564106E-4	4.6592634077293455E-5	0.0	0.10036053380249009	0.0
28	4.1933370669564106E-4	4.6592634077293455E-5	0.0	0.21516478416894116	0.0
29	4.1933370669564106E-4	4.6592634077293455E-5	0.0	0.366451067017913	0.0
30	4.1933370669564106E-4	9.318526815458691E-5	0.0	0.5697347294971443	0.0
31	4.1933370669564106E-4	9.318526815458691E-5	0.0	0.840857267192915	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9730	0.0	21.142859	1
TAGGAGT	150	2.5102054E-10	17.266666	4
ACGTTTA	175	7.2759576E-12	16.914286	26
TCACGTT	180	1.0913936E-11	16.444445	24
GTATTGG	1085	0.0	16.027649	1
CATTGCG	140	3.477362E-8	15.857143	29
CTAATAC	485	0.0	15.257732	3
CGAACTA	355	0.0	15.112677	24
TAGACTG	200	6.184564E-11	14.799999	5
ATAGTAC	315	0.0	14.682539	3
ATTGGAC	1210	0.0	14.524793	3
GTATCAA	14285	0.0	14.41407	2
TAATACT	465	0.0	14.32258	4
CTGTGCG	220	1.8189894E-11	14.295454	9
CCGTTTA	290	0.0	14.034483	27
TCTATAC	145	8.91956E-7	14.034483	3
AGTACCG	215	1.9826984E-10	13.767442	5
TAGGTCG	135	6.572849E-6	13.703704	21
GAGTAAT	420	0.0	13.654763	1
TAATACA	475	0.0	13.631578	4
>>END_MODULE