FastQCFastQC Report
Wed 25 May 2016
SRR2088571_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088571_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747168
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT123101.6475544991220181No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT87091.1656013105486316No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT86631.159444729967022No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37150.49721080131911427No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG21230.28413957771210757No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA19010.254427384470427No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA17800.23823290076662812No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG17540.23475309435093578No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG16220.21708638485588247No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA16070.2150788042314446No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA15170.20303332048481731No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC12030.16100796607991777No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11140.14909632104158638No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA8730.11684119234228448No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA8590.11496745042614245No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT8290.1109522891772667No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7790.10426035376247378No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT7760.10385883763758619No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAT551.9015033E-523.5454549
GCTATCC400.001930557723.1250023
CCGTACA400.001930557723.12500232
CGCACTA400.001930557723.12500229
TACCCCA502.7008765E-422.1999995
TGAACGG502.7008765E-422.19999925
GGTATGA450.003824573220.5555571
TGCGTGC450.003824573220.55555710
TGCGGAT555.1407784E-420.18181832
ACCGTTA555.1407784E-420.1818188
CTAATAC1101.7516868E-920.1818183
GGTATCA32500.020.0938451
GACCGTT656.8979985E-519.9230777
GTATTGG2750.018.8363651
CGTGCGA609.2326937E-418.510
TTCGTTA801.6154363E-518.530
CCGTTTA609.2326937E-418.527
CGTGCAC500.007032635618.4999983
TCTAATA1002.871584E-718.4999982
TGCGACA500.007032635618.49999819
Produced by FastQC (version 0.11.5)