##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088571_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	747168
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.681941678444474	31.0	31.0	34.0	30.0	34.0
2	31.850170242836953	31.0	31.0	34.0	30.0	34.0
3	31.9822034669579	33.0	31.0	34.0	30.0	34.0
4	35.65696603708938	37.0	35.0	37.0	33.0	37.0
5	35.571725502162835	37.0	35.0	37.0	33.0	37.0
6	35.619882007794764	37.0	35.0	37.0	33.0	37.0
7	35.46554054777506	37.0	35.0	37.0	33.0	37.0
8	35.50989871086556	37.0	35.0	37.0	33.0	37.0
9	37.11494469784573	39.0	37.0	39.0	33.0	39.0
10	36.98200137050837	39.0	37.0	39.0	33.0	39.0
11	37.04396333890102	39.0	37.0	39.0	33.0	39.0
12	36.90059799134867	39.0	37.0	39.0	33.0	39.0
13	36.942678219623964	39.0	37.0	39.0	33.0	39.0
14	37.95407057047411	40.0	37.0	41.0	33.0	41.0
15	37.9611948049167	40.0	37.0	41.0	33.0	41.0
16	37.85767993275943	40.0	37.0	41.0	32.0	41.0
17	37.900167030707955	40.0	37.0	41.0	33.0	41.0
18	37.87559558225192	40.0	37.0	41.0	33.0	41.0
19	37.951918444044715	40.0	37.0	41.0	33.0	41.0
20	37.84722311448028	40.0	37.0	41.0	32.0	41.0
21	37.71468799520322	40.0	37.0	41.0	32.0	41.0
22	37.60033486444816	39.0	37.0	41.0	32.0	41.0
23	37.411247537367764	39.0	36.0	41.0	32.0	41.0
24	37.56397891772667	39.0	36.0	41.0	32.0	41.0
25	37.50852954087969	39.0	36.0	41.0	32.0	41.0
26	37.298100293374446	39.0	36.0	41.0	31.0	41.0
27	37.25712021928134	39.0	36.0	41.0	31.0	41.0
28	37.105962782131996	39.0	36.0	41.0	31.0	41.0
29	36.881948905734724	39.0	35.0	41.0	30.0	41.0
30	36.70783143817722	39.0	35.0	41.0	30.0	41.0
31	36.47942765214784	39.0	35.0	40.0	30.0	41.0
32	36.31890284380487	38.0	35.0	40.0	30.0	41.0
33	36.14967718103559	38.0	35.0	40.0	30.0	41.0
34	35.94463494153925	38.0	35.0	40.0	29.0	41.0
35	35.74426902651077	38.0	35.0	40.0	28.0	41.0
36	35.66009117092809	38.0	35.0	40.0	27.0	41.0
37	35.50599463574457	38.0	35.0	40.0	26.0	41.0
38	35.35851642468628	38.0	34.0	40.0	25.0	41.0
39	35.20941341170928	38.0	34.0	40.0	25.0	41.0
40	35.020284594629324	38.0	34.0	40.0	24.0	41.0
41	34.88620765343269	38.0	34.0	40.0	24.0	41.0
42	34.576575816951475	38.0	33.0	40.0	22.0	41.0
43	34.199319296329605	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	1.0
15	5.0
16	7.0
17	20.0
18	46.0
19	109.0
20	267.0
21	490.0
22	894.0
23	1615.0
24	2616.0
25	4044.0
26	6035.0
27	8738.0
28	11689.0
29	15332.0
30	19606.0
31	23952.0
32	28968.0
33	36472.0
34	45013.0
35	55597.0
36	73574.0
37	107368.0
38	139233.0
39	165475.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.54741637757506	21.1768972975288	14.619068268448327	22.656618056447815
2	18.238602295601524	23.837744657158765	35.79449334018587	22.129159707053837
3	19.210806672662642	24.610127842734165	33.51361407340786	22.66545141119534
4	13.11887018716005	16.33849415392522	36.805377103944494	33.73725855497023
5	12.137297100518223	39.140996402415524	34.914369994432306	13.807336502633946
6	31.62541757676988	38.28991605636215	13.771200051394064	16.313466315473896
7	25.15900038545548	34.1164236155724	21.553787100089938	19.17078889888218
8	27.436935200650993	34.506295772838236	19.08539980298942	18.97136922352135
9	24.490476037517666	14.699773009550729	22.07334896569446	38.73640198723714
10	16.215897897126215	28.265396805002357	33.112365625936874	22.406339671934557
11	32.77991027452996	23.12732879352435	23.667100304081544	20.425660627864147
12	21.04867981498137	28.062497323225834	31.045226776307338	19.84359608548546
13	31.69969805987408	21.404021585506875	26.17992205233629	20.716358302282753
14	20.070854212171827	23.31015246905649	28.803160734935112	27.81583258383657
15	22.281735834511114	30.570634716690222	25.748693734207034	21.398935714591634
16	21.24769797421731	28.830597670135766	26.787282110582893	23.13442224506403
17	21.278480877125357	27.454334232729455	27.49247826459377	23.774706625551413
18	22.469511542250203	26.151682084885863	30.18424236584008	21.194564007023857
19	22.356685511156797	25.48329157565635	31.553144674290117	20.60687823889674
20	23.138972761146086	25.239838965266177	30.94712300312647	20.674065270461263
21	21.83779283909375	26.070308150241985	31.582589190115208	20.50930982054906
22	20.740583108484305	26.493104629748597	31.088322840378602	21.677989421388496
23	21.63328729281768	26.45482675917598	31.7346299627393	20.177255985267035
24	21.375246263223264	26.717685982269046	29.937176110325925	21.969891644181764
25	21.313814296115467	26.785006852541866	30.986203905948862	20.91497494539381
26	20.87549252644653	27.830554841749112	30.77781703713221	20.51613559467215
27	20.020664696560882	27.68052164974946	30.508533556040945	21.790280097648722
28	20.45604201464731	27.40561694290976	30.636215683755193	21.50212535868774
29	20.77042914043428	27.271644395905607	29.94507259411538	22.012853869544735
30	20.66442888346396	27.584425457193028	31.541634545376674	20.209511113966336
31	21.40442310163176	27.408293717075676	31.226176709923337	19.961106471369224
32	20.774845817808043	27.2363109769155	30.399990363613007	21.588852841663456
33	20.542100304081544	27.099126300912246	31.080426356589147	21.278347038417063
34	19.83650263394578	27.412308878324552	31.684574285836653	21.066614201893014
35	19.43418347680843	29.329682213371022	30.640632361128954	20.595501948691595
36	21.48552935885905	27.332808685596817	30.363318557539937	20.818343398004195
37	20.551067497537368	27.97443145316716	30.426891943980472	21.047609105315
38	20.259165274744102	26.437427727097518	31.832198381086986	21.471208617071394
39	19.612991991091697	26.379743243822006	32.55225063171871	21.455014133367595
40	18.8307047411024	26.63457214441732	32.2453584735963	22.289364640883978
41	19.840517795194653	25.766895798535266	32.533780889973876	21.8588055162962
42	18.902442288748983	27.369614330378173	31.74921838194355	21.97872499892929
43	18.952364126943337	27.218644267420444	31.420644353077222	22.408347252558997
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	278.0
1	248.0
2	218.0
3	532.0
4	846.0
5	846.0
6	1270.5
7	1695.0
8	1827.5
9	1960.0
10	2990.0
11	4020.0
12	4020.0
13	7067.0
14	10114.0
15	16940.0
16	23766.0
17	21647.5
18	19529.0
19	19529.0
20	19558.5
21	19588.0
22	15718.0
23	11848.0
24	13164.0
25	14480.0
26	14480.0
27	16206.0
28	17932.0
29	19068.5
30	20205.0
31	21923.5
32	23642.0
33	23642.0
34	26020.0
35	28398.0
36	30950.5
37	33503.0
38	35263.5
39	37024.0
40	37024.0
41	38289.0
42	39554.0
43	41695.5
44	43837.0
45	46849.0
46	49861.0
47	49861.0
48	63424.0
49	76987.0
50	71333.5
51	65680.0
52	59351.0
53	53022.0
54	53022.0
55	47365.0
56	41708.0
57	35569.5
58	29431.0
59	26023.0
60	22615.0
61	22615.0
62	19814.5
63	17014.0
64	14767.5
65	12521.0
66	10772.5
67	9024.0
68	9024.0
69	7568.0
70	6112.0
71	5170.5
72	4229.0
73	3471.5
74	2714.0
75	2714.0
76	2192.5
77	1671.0
78	1315.5
79	960.0
80	754.5
81	549.0
82	549.0
83	427.5
84	306.0
85	234.5
86	163.0
87	126.5
88	90.0
89	90.0
90	68.0
91	46.0
92	32.0
93	18.0
94	13.5
95	9.0
96	9.0
97	5.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	747168.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.21586328735729
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.18053765540408	50.44039072545997
2	8.423015235841621	9.975522373458498
3	2.421402602478108	4.301563364159843
4	1.1692950381971787	2.769632604978774
5	0.6389315593850683	1.89174419352621
6	0.4294316321727212	1.5257498893203933
7	0.29455393111595934	1.220958572100141
8	0.20958489793492346	0.9928600530567324
9	0.1737354214180584	0.9259103666576834
>10	0.9447287060121554	10.279184648851256
>50	0.06656089495594242	2.7338155272507025
>100	0.04052494878026123	4.616578321171575
>500	0.004754323599509713	1.888673347241744
>1k	0.002263963618814149	2.4581711027132753
>5k	4.5279272376282987E-4	2.328934794739039
>10k+	2.2639636188141494E-4	1.6503101153141588
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12310	1.6475544991220181	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8709	1.1656013105486316	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8663	1.159444729967022	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3715	0.49721080131911427	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2123	0.28413957771210757	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1901	0.254427384470427	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1780	0.23823290076662812	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1754	0.23475309435093578	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1622	0.21708638485588247	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1607	0.2150788042314446	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1517	0.20303332048481731	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1203	0.16100796607991777	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1114	0.14909632104158638	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	873	0.11684119234228448	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	859	0.11496745042614245	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	829	0.1109522891772667	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	779	0.10426035376247378	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	776	0.10385883763758619	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	1.338387082958585E-4	0.0	0.0	4.0151612488757547E-4	0.0
11	1.338387082958585E-4	0.0	0.0	6.691935414792925E-4	0.0
12	1.338387082958585E-4	0.0	0.0	0.0012045483746627264	0.0
13	1.338387082958585E-4	0.0	0.0	0.0012045483746627264	0.0
14	2.67677416591717E-4	0.0	0.0	0.0016060644995503019	0.0
15	2.67677416591717E-4	0.0	0.0	0.0022752580410295943	0.0
16	2.67677416591717E-4	0.0	0.0	0.003747483832284038	0.0
17	6.691935414792925E-4	0.0	0.0	0.0054873870401301985	0.0
18	9.368709580710095E-4	0.0	0.0	0.005755064456721915	0.0
19	9.368709580710095E-4	0.0	0.0	0.007227290247976359	0.0
20	9.368709580710095E-4	0.0	0.0	0.011643967621739689	0.0
21	9.368709580710095E-4	0.0	0.0	0.01619448370379888	0.0
22	9.368709580710095E-4	0.0	0.0	0.02489399974302968	0.0
23	9.368709580710095E-4	0.0	0.0	0.029176838408497153	0.0
24	9.368709580710095E-4	0.0	0.0	0.03801019315602381	0.0
25	9.368709580710095E-4	0.0	0.0	0.04242687052978714	0.0
26	9.368709580710095E-4	0.0	0.0	0.05005567690265108	0.0
27	9.368709580710095E-4	0.0	0.0	0.10144974088826074	0.0
28	9.368709580710095E-4	0.0	0.0	0.23903593301640327	0.0
29	9.368709580710095E-4	0.0	0.0	0.43484196325324426	0.0
30	9.368709580710095E-4	0.0	0.0	0.714966379716476	0.0
31	9.368709580710095E-4	0.0	0.0	1.0756616985738148	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTTAT	55	1.9015033E-5	23.545454	9
GCTATCC	40	0.0019305577	23.125002	3
CCGTACA	40	0.0019305577	23.125002	32
CGCACTA	40	0.0019305577	23.125002	29
TACCCCA	50	2.7008765E-4	22.199999	5
TGAACGG	50	2.7008765E-4	22.199999	25
GGTATGA	45	0.0038245732	20.555557	1
TGCGTGC	45	0.0038245732	20.555557	10
TGCGGAT	55	5.1407784E-4	20.181818	32
ACCGTTA	55	5.1407784E-4	20.181818	8
CTAATAC	110	1.7516868E-9	20.181818	3
GGTATCA	3250	0.0	20.093845	1
GACCGTT	65	6.8979985E-5	19.923077	7
GTATTGG	275	0.0	18.836365	1
CGTGCGA	60	9.2326937E-4	18.5	10
TTCGTTA	80	1.6154363E-5	18.5	30
CCGTTTA	60	9.2326937E-4	18.5	27
CGTGCAC	50	0.0070326356	18.499998	3
TCTAATA	100	2.871584E-7	18.499998	2
TGCGACA	50	0.0070326356	18.499998	19
>>END_MODULE