FastQCFastQC Report
Wed 25 May 2016
SRR2088570_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088570_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2301795
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT332751.445610925386492No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT237781.0330198823092411No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT230621.0019137238546438No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97410.4231914657908285No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG53660.2331224109879464No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA43670.18972149995981397No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA42700.18550739748761294No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG42430.184334399892258No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG40700.17681852641090975No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA37000.16074411491900886No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA36570.1588760076375177No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC30020.13041995486131475No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29150.12664029594294887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCTA502.7227907E-729.59999810
GGTATCA83350.021.3743251
TAATACT5750.018.0173914
TTCGCTA1258.591087E-917.76000226
CTAATAC4600.017.2934783
ATTGGAC8500.016.323533
GTATTGG7950.015.5911961
TTACCCG1557.219569E-915.51612910
GTATTAG4550.015.4505491
GGACCCT9400.015.1542566
TCGGGTA1101.4530469E-515.13636425
ATAGACG1252.9616822E-614.8000013
TATACTG3000.014.7999995
TTAACGG2400.014.64583435
TATACAG3550.014.5915495
TATACAC12700.014.4212637
ATGCGAC1551.2117016E-714.3225821
TACCCGA1702.4399924E-814.14705911
TGGACCC9950.014.1306535
GAGTCGC1203.3031858E-513.87500123
Produced by FastQC (version 0.11.5)