##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088570_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2301795
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59114517148573	31.0	31.0	34.0	30.0	34.0
2	31.75629019960509	31.0	31.0	34.0	30.0	34.0
3	31.89166194209302	31.0	31.0	34.0	30.0	34.0
4	35.58225645637427	37.0	35.0	37.0	33.0	37.0
5	35.48353524097498	37.0	35.0	37.0	33.0	37.0
6	35.52857009420909	37.0	35.0	37.0	33.0	37.0
7	35.38697190670759	37.0	35.0	37.0	33.0	37.0
8	35.421070512361005	37.0	35.0	37.0	33.0	37.0
9	37.0108063489581	39.0	37.0	39.0	33.0	39.0
10	36.85195944903869	39.0	37.0	39.0	32.0	39.0
11	36.92468225884581	39.0	37.0	39.0	33.0	39.0
12	36.76654784635469	39.0	37.0	39.0	32.0	39.0
13	36.82132726850132	39.0	37.0	39.0	32.0	39.0
14	37.82465336834948	40.0	37.0	41.0	32.0	41.0
15	37.83403387356389	40.0	37.0	41.0	32.0	41.0
16	37.720353028831845	40.0	37.0	41.0	32.0	41.0
17	37.74462712795883	40.0	37.0	41.0	32.0	41.0
18	37.71290101855291	40.0	37.0	41.0	32.0	41.0
19	37.775801928494936	40.0	37.0	41.0	32.0	41.0
20	37.67312553898154	40.0	37.0	41.0	32.0	41.0
21	37.53664509654422	39.0	37.0	41.0	32.0	41.0
22	37.41966117747236	39.0	36.0	41.0	32.0	41.0
23	37.2439583021077	39.0	36.0	41.0	31.0	41.0
24	37.39324092718943	39.0	36.0	41.0	31.0	41.0
25	37.346202854728595	39.0	36.0	41.0	31.0	41.0
26	37.13206388926903	39.0	36.0	41.0	31.0	41.0
27	37.09054672549033	39.0	36.0	41.0	31.0	41.0
28	36.94385077732813	39.0	36.0	41.0	31.0	41.0
29	36.72378382957648	39.0	35.0	41.0	30.0	41.0
30	36.57751798053258	39.0	35.0	40.0	30.0	41.0
31	36.35328776020454	38.0	35.0	40.0	30.0	41.0
32	36.20125336965281	38.0	35.0	40.0	30.0	41.0
33	36.03328011399799	38.0	35.0	40.0	29.0	41.0
34	35.83711364391703	38.0	35.0	40.0	28.0	41.0
35	35.64483457475579	38.0	35.0	40.0	27.0	41.0
36	35.587886410388414	38.0	35.0	40.0	27.0	41.0
37	35.44714929001062	38.0	34.0	40.0	26.0	41.0
38	35.31339584976073	38.0	34.0	40.0	25.0	41.0
39	35.17755490823466	38.0	34.0	40.0	25.0	41.0
40	35.010720329134436	38.0	34.0	40.0	24.0	41.0
41	34.89314600127292	38.0	34.0	40.0	24.0	41.0
42	34.62175997428094	38.0	33.0	40.0	23.0	41.0
43	34.23875279944565	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	6.0
15	9.0
16	28.0
17	76.0
18	171.0
19	407.0
20	865.0
21	1773.0
22	3261.0
23	5685.0
24	9153.0
25	13814.0
26	20231.0
27	27817.0
28	38248.0
29	49331.0
30	62338.0
31	76743.0
32	93392.0
33	114302.0
34	140472.0
35	174677.0
36	226578.0
37	327215.0
38	438832.0
39	476367.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.27487460872927	21.338520589366127	14.267386974078924	23.119217827825675
2	18.463025595241973	23.88279581804635	35.28789488203771	22.36628370467396
3	19.28812079268571	24.763760456513285	33.02387918993655	22.924239560864457
4	13.481739251323424	16.62406947621313	36.6918861149668	33.202305157496646
5	12.435729506754512	39.0488292832333	34.88056060596187	13.634880604050318
6	31.849100376010895	38.386911084610055	13.960191937162083	15.803796602216966
7	25.589507319287776	33.80062081983843	21.66252859181639	18.94734326905741
8	27.235787722190725	34.681976457503815	19.22217226121353	18.860063559091927
9	24.89431074444075	14.490560627684046	21.645368071439897	38.969760556435304
10	16.201573120108435	28.245086986460567	33.23497531274505	22.31836458068594
11	33.456498080845606	22.989015094741276	23.18859846337315	20.36588836103997
12	21.183815239845423	27.666712283239818	30.923170829722018	20.226301647192734
13	31.443069430596555	21.294337679941087	25.960348336841466	21.302244552620888
14	20.44374064588723	22.78747673011715	28.436676593701872	28.332106030293748
15	22.851948153506285	30.062147150376116	25.218622857378698	21.867281838738897
16	21.667959136239325	28.522218529452015	26.445882452607638	23.36393988170102
17	21.599968720064126	27.323067432156208	27.384063307114666	23.692900540665
18	22.54410145125869	26.06926333578794	29.718849854135577	21.66778535881779
19	22.61083198112777	25.510047593291326	30.961141196327212	20.917979229253692
20	23.313240318968457	25.29465047929985	30.25577864232045	21.136330559411242
21	22.15227681005476	26.005660799506476	30.860393736192844	20.981668654245926
22	21.460121340084587	26.41786084338527	30.245873329293012	21.876144487237134
23	22.025940624599496	26.366248949189654	30.76485959870449	20.84295082750636
24	21.830918913282897	26.611839890172668	29.3479653922265	22.209275804317937
25	21.924584943489755	26.382584026813856	30.323421503652586	21.369409526043807
26	21.325704504527987	27.33023575079449	30.268203727960135	21.07585601671739
27	20.73260216483223	27.263331443503873	29.966352346755464	22.03771404490843
28	21.084718665215625	27.116011634398372	30.00879748196516	21.79047221842084
29	21.300202667917866	27.064660406335054	29.51679015724684	22.118346768500235
30	21.20314797799109	27.321764101494704	30.792099209529955	20.682988710984255
31	21.862416070935943	27.037855239063425	30.44636902938793	20.653359660612697
32	21.32970138522327	27.02977458896209	29.721499959813976	21.919024066000663
33	21.021941571686444	26.9132568278235	30.34748967653505	21.717311923955002
34	20.62003784003354	27.086382584026815	30.967223406080908	21.32635616985874
35	20.287949187481942	28.580520854376694	30.076961675561897	21.054568282579464
36	21.821665265586205	27.009181964510304	29.864735999513425	21.304416770390063
37	21.007387712632966	27.418601569644558	29.950625490106635	21.623385227615838
38	20.73829337538747	26.360079850725192	30.961792861657965	21.93983391222937
39	20.36236936825391	26.16605735958241	31.68457660217352	21.78699666999016
40	19.645494060070508	26.4003962125211	31.53925523341566	22.41485449399273
41	20.361152926303166	25.52468834105557	31.828855306402176	22.285303426239086
42	19.606958916845333	26.97998735769258	31.133311176712088	22.279742548749997
43	19.642366066482897	26.602325576343677	30.80921628555106	22.946092071622363
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	858.0
1	742.0
2	626.0
3	1379.0
4	2132.0
5	2132.0
6	3325.0
7	4518.0
8	4804.5
9	5091.0
10	7587.0
11	10083.0
12	10083.0
13	18119.0
14	26155.0
15	44443.0
16	62731.0
17	57612.0
18	52493.0
19	52493.0
20	53228.5
21	53964.0
22	44014.0
23	34064.0
24	38629.5
25	43195.0
26	43195.0
27	48355.5
28	53516.0
29	58114.0
30	62712.0
31	68470.5
32	74229.0
33	74229.0
34	82398.5
35	90568.0
36	98076.5
37	105585.0
38	112475.5
39	119366.0
40	119366.0
41	123588.5
42	127811.0
43	134042.5
44	140274.0
45	146997.5
46	153721.0
47	153721.0
48	187635.5
49	221550.0
50	207859.0
51	194168.0
52	177904.0
53	161640.0
54	161640.0
55	146624.5
56	131609.0
57	114966.5
58	98324.0
59	88169.0
60	78014.0
61	78014.0
62	68640.0
63	59266.0
64	51638.0
65	44010.0
66	37526.5
67	31043.0
68	31043.0
69	26317.5
70	21592.0
71	18009.5
72	14427.0
73	11901.5
74	9376.0
75	9376.0
76	7658.0
77	5940.0
78	4600.5
79	3261.0
80	2554.0
81	1847.0
82	1847.0
83	1433.0
84	1019.0
85	778.0
86	537.0
87	387.0
88	237.0
89	237.0
90	194.5
91	152.0
92	103.0
93	54.0
94	40.0
95	26.0
96	26.0
97	17.0
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2301795.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.65270899484471
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.1619544925644	41.405821078624136
2	11.08783589070425	11.454335212902828
3	3.6027421061560942	5.58274168778264
4	1.5522602435420143	3.207137865757698
5	0.8171044030219267	2.1102827973848943
6	0.5247663379577128	1.626336176689204
7	0.35306090930528117	1.2765586684060664
8	0.2667815593736135	1.1023992201212913
9	0.20097294385154202	0.9342717286140868
>10	1.2499149376880303	12.164460489483483
>50	0.11013610533015285	3.9375412683545377
>100	0.06420563964493106	6.1701193935397
>500	0.004638200992803533	1.6183117100774493
>1k	0.003204575231391491	3.261887761020633
>5k	1.6866185428376267E-4	0.6580489611364069
>10k+	2.5299278142564403E-4	3.489745980104802
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	33275	1.445610925386492	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23778	1.0330198823092411	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	23062	1.0019137238546438	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9741	0.4231914657908285	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5366	0.2331224109879464	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4367	0.18972149995981397	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	4270	0.18550739748761294	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4243	0.184334399892258	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4070	0.17681852641090975	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3700	0.16074411491900886	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3657	0.1588760076375177	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3002	0.13041995486131475	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2915	0.12664029594294887	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	4.344435538351591E-5	0.0
7	0.0	0.0	0.0	4.344435538351591E-5	0.0
8	0.0	0.0	0.0	8.688871076703182E-5	0.0
9	0.0	0.0	0.0	2.1722177691757953E-4	0.0
10	0.0	0.0	0.0	2.6066613230109543E-4	0.0
11	4.344435538351591E-5	0.0	0.0	4.77887909218675E-4	0.0
12	4.344435538351591E-5	0.0	0.0	6.516653307527386E-4	0.0
13	4.344435538351591E-5	0.0	0.0	6.516653307527386E-4	0.0
14	4.344435538351591E-5	0.0	0.0	7.819983969032864E-4	0.0
15	4.344435538351591E-5	0.0	0.0	9.5577581843735E-4	0.0
16	8.688871076703182E-5	0.0	0.0	0.0011729975953549295	0.0
17	8.688871076703182E-5	0.0	0.0	0.0017812185707241522	4.344435538351591E-5
18	8.688871076703182E-5	0.0	0.0	0.0021722177691757953	4.344435538351591E-5
19	1.3033306615054771E-4	0.0	4.344435538351591E-5	0.0030845492322296294	4.344435538351591E-5
20	1.3033306615054771E-4	0.0	4.344435538351591E-5	0.005039545224487845	4.344435538351591E-5
21	1.3033306615054771E-4	0.0	4.344435538351591E-5	0.00838476058901857	4.344435538351591E-5
22	1.3033306615054771E-4	0.0	4.344435538351591E-5	0.01433663727656025	4.344435538351591E-5
23	1.3033306615054771E-4	0.0	4.344435538351591E-5	0.017681852641090973	4.344435538351591E-5
24	1.3033306615054771E-4	0.0	4.344435538351591E-5	0.022026288179442566	4.344435538351591E-5
25	1.3033306615054771E-4	0.0	4.344435538351591E-5	0.02515428176705571	4.344435538351591E-5
26	1.3033306615054771E-4	0.0	4.344435538351591E-5	0.031019269743830357	4.344435538351591E-5
27	1.3033306615054771E-4	0.0	4.344435538351591E-5	0.07307340575507376	4.344435538351591E-5
28	1.3033306615054771E-4	0.0	4.344435538351591E-5	0.18020718613082398	4.344435538351591E-5
29	1.3033306615054771E-4	0.0	4.344435538351591E-5	0.34160296638058557	4.344435538351591E-5
30	1.3033306615054771E-4	0.0	4.344435538351591E-5	0.5530466440321575	4.344435538351591E-5
31	1.3033306615054771E-4	0.0	4.344435538351591E-5	0.8577219083367545	4.344435538351591E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGCTA	50	2.7227907E-7	29.599998	10
GGTATCA	8335	0.0	21.374325	1
TAATACT	575	0.0	18.017391	4
TTCGCTA	125	8.591087E-9	17.760002	26
CTAATAC	460	0.0	17.293478	3
ATTGGAC	850	0.0	16.32353	3
GTATTGG	795	0.0	15.591196	1
TTACCCG	155	7.219569E-9	15.516129	10
GTATTAG	455	0.0	15.450549	1
GGACCCT	940	0.0	15.154256	6
TCGGGTA	110	1.4530469E-5	15.136364	25
ATAGACG	125	2.9616822E-6	14.800001	3
TATACTG	300	0.0	14.799999	5
TTAACGG	240	0.0	14.645834	35
TATACAG	355	0.0	14.591549	5
TATACAC	1270	0.0	14.42126	37
ATGCGAC	155	1.2117016E-7	14.32258	21
TACCCGA	170	2.4399924E-8	14.147059	11
TGGACCC	995	0.0	14.130653	5
GAGTCGC	120	3.3031858E-5	13.875001	23
>>END_MODULE