FastQCFastQC Report
Wed 25 May 2016
SRR2088569_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088569_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2466055
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT357821.4509814257995057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT253731.0288902721147746No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT251631.0203746469563737No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105260.42683557341584033No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG60260.2443578914501096No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA48280.19577827745123283No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA47860.1940751524195527No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG47480.19253422977184206No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA42900.17396205680732993No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA36380.14752306822029515No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG35400.14354910981304148No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT33470.13572284478651125No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC27680.11224404970692056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83400.022.4262581
GTATTGG7050.019.418441
ATTGGAC7450.018.624163
TTGGACC9450.018.5978834
TATACTG3600.018.55
TGGACCC9600.017.343755
GGACCCT9500.016.5526316
GACCCTC9600.016.3802077
ACGTTTA1151.2435303E-616.08695626
CGCTAGA700.002593622515.8571431
ACCCTCG10250.015.1609758
CAATGCG1508.112693E-814.819
TACCGAC3250.014.87
CGACGAG2400.014.64583324
CAATTCG1551.2118107E-714.32258115
GCGACTA1304.448646E-614.23076935
TATACAC15450.013.88996937
GTATCAA135250.013.869872
CGTATAC1356.573804E-613.7037043
CGACTAT1356.573804E-613.70370436
Produced by FastQC (version 0.11.5)