FastQCFastQC Report
Wed 25 May 2016
SRR2088568_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088568_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1273464
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT319052.5053711765703626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT258732.0317025059208587No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT187471.472126420534856No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92880.7293492395544751No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG63250.4966767808120214No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA56930.44704836571744466No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG51560.40487991808170465No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA44920.3527386718430988No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG41410.3251760552320286No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA34970.2746053284584409No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31220.24515808848934875No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA29270.2298455237054208No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC25480.200084179843325No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT21810.1712651476602401No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC21150.16608243342567988No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT20320.15956477764585414No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18890.14833556347097365No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA18300.14370253104916983No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG17840.14009033627962786No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA17550.1378130830553514No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT17180.13490762204506762No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16800.13192363506153296No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG16700.13113837532902384No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA15930.12509187538870356No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC15560.12218641437841982No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA15250.11975210920764152No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT15120.11873127155537966No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA15050.11818158974262326No Hit
TTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCC14830.11645401833110319No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14670.11519760275908861No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14020.11009341449777928No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG12880.10114145354717527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67300.028.2310541
CGCGATA502.702325E-422.19999914
GTATCAA102150.018.5814972
TTGGACC6800.017.6838254
GTATTAG953.607005E-617.5263161
ATTGGAC4650.017.5053773
CGATTTA752.0674868E-417.26666630
TGGACCC6650.016.4135345
GATACCT6000.016.34166736
CGAATCA803.3829254E-416.187529
GATACTG6050.015.90082636
ACGTGCG700.002592699815.8571429
GACTTTT4600.015.6847814
GTATTGG4150.015.6024091
AGGTCCG957.06063E-515.5789485
TGATACT6400.015.320312535
TAAGCCG855.3647166E-415.23529410
ACACTTA1101.4520336E-515.1363646
TTGCGAT1353.9728184E-715.07407311
ACTCTGT6900.015.01449337
Produced by FastQC (version 0.11.5)