##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088568_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1273464
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.89606145128563	31.0	31.0	31.0	28.0	34.0
2	31.045362098967853	31.0	31.0	34.0	28.0	34.0
3	31.022888750683176	31.0	31.0	34.0	27.0	34.0
4	34.832894373142864	35.0	35.0	37.0	32.0	37.0
5	34.67590053586124	35.0	35.0	37.0	32.0	37.0
6	34.89381325267146	35.0	35.0	37.0	32.0	37.0
7	34.7135741567881	35.0	35.0	37.0	32.0	37.0
8	34.86992722212799	35.0	35.0	37.0	32.0	37.0
9	36.29193758127438	38.0	35.0	39.0	32.0	39.0
10	36.045656571367545	38.0	35.0	39.0	31.0	39.0
11	36.207992530609424	38.0	35.0	39.0	32.0	39.0
12	35.92669835974947	37.0	35.0	39.0	30.0	39.0
13	36.08642882719889	37.0	35.0	39.0	31.0	39.0
14	36.746883304121674	38.0	36.0	40.0	31.0	41.0
15	36.83245698347185	38.0	36.0	40.0	31.0	41.0
16	36.617938944485275	38.0	36.0	40.0	31.0	41.0
17	36.7673432464522	38.0	36.0	40.0	31.0	41.0
18	36.75828370491824	38.0	36.0	40.0	31.0	41.0
19	36.860051795731955	39.0	36.0	40.0	31.0	41.0
20	36.75750237148439	39.0	36.0	40.0	30.0	41.0
21	36.60037896634691	38.0	35.0	40.0	30.0	41.0
22	36.44939315127872	38.0	35.0	40.0	30.0	41.0
23	36.254010321453926	38.0	35.0	40.0	30.0	41.0
24	36.3734758108592	38.0	35.0	40.0	30.0	41.0
25	36.274709768002865	38.0	35.0	40.0	30.0	41.0
26	35.96712667181797	38.0	35.0	40.0	29.0	41.0
27	35.8873552766313	38.0	34.0	40.0	29.0	41.0
28	35.68813723827293	38.0	34.0	40.0	27.0	41.0
29	35.44684576870646	38.0	34.0	40.0	27.0	41.0
30	35.24143988365592	38.0	34.0	40.0	27.0	41.0
31	34.870388954850704	37.0	33.0	40.0	25.0	41.0
32	34.69840215349629	37.0	33.0	40.0	25.0	41.0
33	34.450110093414494	37.0	33.0	40.0	25.0	41.0
34	34.18168632957037	36.0	33.0	40.0	24.0	41.0
35	33.90979721452668	36.0	33.0	39.0	23.0	40.0
36	33.81243521607207	36.0	33.0	39.0	23.0	41.0
37	33.590973125270914	36.0	32.0	39.0	21.0	41.0
38	33.401520576946034	36.0	32.0	39.0	21.0	41.0
39	33.15453833009806	36.0	31.0	39.0	19.0	40.0
40	32.88597793106048	36.0	31.0	39.0	18.0	40.0
41	32.668206561002116	36.0	31.0	39.0	15.0	40.0
42	32.284192564532646	35.0	30.0	39.0	15.0	40.0
43	31.83492505481113	35.0	30.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	1.0
14	11.0
15	26.0
16	62.0
17	118.0
18	292.0
19	595.0
20	1174.0
21	2284.0
22	3850.0
23	5988.0
24	9292.0
25	13452.0
26	18586.0
27	24520.0
28	32021.0
29	40382.0
30	49092.0
31	58068.0
32	69418.0
33	82897.0
34	100773.0
35	123178.0
36	162074.0
37	202857.0
38	191984.0
39	80467.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.71584591319425	24.27716841622535	16.579502836358152	19.42748283422225
2	22.652701607583726	26.007723814728962	31.48703065025788	19.852543927429437
3	23.27698309492848	24.30904996136522	33.4165708649793	18.997396078727
4	16.823954191088244	18.508179265373816	32.54650308135919	32.12136346217875
5	11.463378627114706	41.661405426458856	33.33356891125309	13.541647035173355
6	32.311474843419205	37.98034337837583	11.928880596546113	17.779301181658845
7	23.636396474497907	36.24240653838663	20.01894046474812	20.102256522367338
8	28.784873384720733	35.16283145813309	17.497864093527575	18.554431063618605
9	24.175084650999164	14.088109283026453	22.24373833889297	39.49306772708141
10	17.374578315523642	29.630205486766805	30.868717136880196	22.12649906082936
11	34.02671767713889	22.275619884032842	22.73845197037372	20.959210468454543
12	20.61024104332749	29.622666993334718	30.859765175929592	18.907326787408202
13	34.40466318639553	20.91044583906573	26.214718280218364	18.470172694320375
14	19.58343541709856	23.934324017011868	29.490664832299935	26.99157573358964
15	22.251041254405308	31.17913030914105	26.17828222862994	20.391546207823698
16	20.28043195567366	28.74969374870432	28.0679312489399	22.90194304668212
17	21.267110809571374	27.402737729531417	27.902162919407225	23.427988541489984
18	22.408093200907132	24.650088263193933	31.349296093175777	21.592522442723155
19	22.432436252614917	23.955683081736115	33.782501900328555	19.829378765320417
20	23.23308707588122	23.433328307671044	33.490149701915406	19.84343491453233
21	22.00886715289949	24.22447748817399	34.51208671780278	19.254568641123736
22	19.892121017947897	24.85339200794055	33.879717055213185	21.374769918898377
23	21.453845573962045	25.10514627818297	34.574357814590755	18.866650333264232
24	21.01700558476722	25.062506674707723	32.460674192595945	21.459813547929112
25	20.850294943555532	25.80237839467782	33.29509118436014	20.05223547740651
26	20.429081623037636	26.825885930030218	33.236118178448706	19.508914268483444
27	19.410285646080297	26.48414089444224	33.089196082496244	21.01637737698121
28	20.045403717733677	26.393050765471187	33.07466877744483	20.486876739350308
29	19.9655428029375	25.565151429486814	32.07267735876319	22.3966284088125
30	20.34796429266944	26.198227825835673	34.20371522084645	19.250092660648434
31	21.377282750042404	26.19838487778218	33.30278672973873	19.121545642436693
32	20.84409139166871	26.047929113033426	32.36974111557139	20.73823837972648
33	20.470700388860617	26.239375435819152	32.76739664411401	20.522527531206222
34	19.28974827714015	26.82769202741499	33.85560958142515	20.026950114019712
35	18.567230797258503	28.982052103553773	32.80524616322095	19.64547093596678
36	21.742507051632398	25.96359221776195	32.36762091429361	19.926279816312043
37	20.395472506486247	27.680248519000145	31.934079015975325	19.990199958538284
38	20.439839681373012	25.22615480296263	33.60738897997902	20.726616535685345
39	19.578959436623258	25.453094865657768	34.36045306345527	20.607492634263707
40	18.225721339590283	25.85067186822714	33.59765175929591	22.325955032886675
41	19.86071062864753	25.031410389300362	33.874220237085616	21.233658744966487
42	19.12814182418977	26.998800123128724	32.50590515318847	21.367152899493036
43	19.06084506511374	27.109757323332268	31.84746486747957	21.98193274407443
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	292.0
1	290.5
2	289.0
3	542.5
4	796.0
5	796.0
6	1620.0
7	2444.0
8	2826.5
9	3209.0
10	5127.0
11	7045.0
12	7045.0
13	14361.5
14	21678.0
15	39617.0
16	57556.0
17	50101.0
18	42646.0
19	42646.0
20	45690.0
21	48734.0
22	36151.0
23	23568.0
24	24712.0
25	25856.0
26	25856.0
27	26769.5
28	27683.0
29	28378.5
30	29074.0
31	30906.0
32	32738.0
33	32738.0
34	35882.5
35	39027.0
36	42928.0
37	46829.0
38	49468.5
39	52108.0
40	52108.0
41	55017.5
42	57927.0
43	61448.0
44	64969.0
45	72292.5
46	79616.0
47	79616.0
48	113797.5
49	147979.0
50	134212.5
51	120446.0
52	106414.5
53	92383.0
54	92383.0
55	79921.5
56	67460.0
57	58084.5
58	48709.0
59	43304.5
60	37900.0
61	37900.0
62	33308.5
63	28717.0
64	24995.0
65	21273.0
66	18119.0
67	14965.0
68	14965.0
69	12922.5
70	10880.0
71	9147.0
72	7414.0
73	6078.5
74	4743.0
75	4743.0
76	3802.0
77	2861.0
78	2251.0
79	1641.0
80	1317.0
81	993.0
82	993.0
83	734.5
84	476.0
85	362.5
86	249.0
87	201.5
88	154.0
89	154.0
90	120.5
91	87.0
92	56.5
93	26.0
94	20.0
95	14.0
96	14.0
97	10.0
98	6.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1273464.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.1396342217985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.75000285884731	42.829445122760305
2	9.153181544196336	9.361807122718346
3	2.663651859788944	4.086545454114597
4	1.2387149379786668	2.533897153332273
5	0.7117478107618341	1.8199261350263025
6	0.4626194938017288	1.4194915018136394
7	0.3185834498792674	1.1404568767161087
8	0.22443922235701458	0.91821917890901
9	0.18304060317338855	0.8424566544622014
>10	1.1313253415472728	10.974224519917202
>50	0.09541078595878612	3.3882031204770775
>100	0.05539970707290246	5.458136581440436
>500	0.005092450558538494	1.8744362878647571
>1k	0.0057097172929067965	5.225330495469399
>5k	6.172667343683023E-4	2.0883013240770123
>10k+	4.6295005077622665E-4	6.03912247090142
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	31905	2.5053711765703626	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	25873	2.0317025059208587	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	18747	1.472126420534856	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9288	0.7293492395544751	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6325	0.4966767808120214	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5693	0.44704836571744466	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5156	0.40487991808170465	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4492	0.3527386718430988	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4141	0.3251760552320286	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3497	0.2746053284584409	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3122	0.24515808848934875	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2927	0.2298455237054208	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2548	0.200084179843325	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2181	0.1712651476602401	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	2115	0.16608243342567988	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	2032	0.15956477764585414	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1889	0.14833556347097365	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1830	0.14370253104916983	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	1784	0.14009033627962786	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1755	0.1378130830553514	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	1718	0.13490762204506762	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1680	0.13192363506153296	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1670	0.13113837532902384	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1593	0.12509187538870356	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	1556	0.12218641437841982	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1525	0.11975210920764152	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT	1512	0.11873127155537966	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	1505	0.11818158974262326	No Hit
TTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCC	1483	0.11645401833110319	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1467	0.11519760275908861	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1402	0.11009341449777928	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	1288	0.10114145354717527	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	1.5705194650182495E-4
6	7.852597325091248E-5	0.0	0.0	0.0	1.5705194650182495E-4
7	7.852597325091248E-5	0.0	0.0	0.0	1.5705194650182495E-4
8	7.852597325091248E-5	0.0	0.0	0.0	1.5705194650182495E-4
9	7.852597325091248E-5	0.0	0.0	7.852597325091248E-5	1.5705194650182495E-4
10	1.5705194650182495E-4	0.0	0.0	1.5705194650182495E-4	1.5705194650182495E-4
11	1.5705194650182495E-4	0.0	0.0	2.355779197527374E-4	1.5705194650182495E-4
12	1.5705194650182495E-4	0.0	0.0	3.141038930036499E-4	1.5705194650182495E-4
13	1.5705194650182495E-4	0.0	0.0	3.141038930036499E-4	1.5705194650182495E-4
14	1.5705194650182495E-4	0.0	0.0	3.141038930036499E-4	1.5705194650182495E-4
15	1.5705194650182495E-4	0.0	0.0	3.9262986625456236E-4	1.5705194650182495E-4
16	1.5705194650182495E-4	0.0	0.0	5.496818127563873E-4	1.5705194650182495E-4
17	1.5705194650182495E-4	0.0	0.0	0.0012564155720145996	1.5705194650182495E-4
18	1.5705194650182495E-4	0.0	0.0	0.001491993491767337	1.5705194650182495E-4
19	1.5705194650182495E-4	0.0	0.0	0.0021202012777746366	1.5705194650182495E-4
20	2.355779197527374E-4	0.0	0.0	0.005104188261309311	1.5705194650182495E-4
21	3.141038930036499E-4	0.0	0.0	0.009109012897105846	1.5705194650182495E-4
22	3.141038930036499E-4	0.0	0.0	0.016647506329193443	1.5705194650182495E-4
23	3.141038930036499E-4	0.0	7.852597325091248E-5	0.02065233096498998	1.5705194650182495E-4
24	3.141038930036499E-4	0.0	7.852597325091248E-5	0.025206837413542904	2.355779197527374E-4
25	3.141038930036499E-4	0.0	7.852597325091248E-5	0.02881903218308488	2.355779197527374E-4
26	3.141038930036499E-4	0.0	7.852597325091248E-5	0.034551428230401485	2.355779197527374E-4
27	3.141038930036499E-4	0.0	7.852597325091248E-5	0.07428557069536319	2.355779197527374E-4
28	3.141038930036499E-4	0.0	7.852597325091248E-5	0.17927479693183318	2.355779197527374E-4
29	3.141038930036499E-4	0.0	7.852597325091248E-5	0.3406456719624583	2.355779197527374E-4
30	3.141038930036499E-4	0.0	7.852597325091248E-5	0.5715905592933919	2.355779197527374E-4
31	3.141038930036499E-4	0.0	7.852597325091248E-5	0.8545196409164295	2.355779197527374E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6730	0.0	28.231054	1
CGCGATA	50	2.702325E-4	22.199999	14
GTATCAA	10215	0.0	18.581497	2
TTGGACC	680	0.0	17.683825	4
GTATTAG	95	3.607005E-6	17.526316	1
ATTGGAC	465	0.0	17.505377	3
CGATTTA	75	2.0674868E-4	17.266666	30
TGGACCC	665	0.0	16.413534	5
GATACCT	600	0.0	16.341667	36
CGAATCA	80	3.3829254E-4	16.1875	29
GATACTG	605	0.0	15.900826	36
ACGTGCG	70	0.0025926998	15.857142	9
GACTTTT	460	0.0	15.684781	4
GTATTGG	415	0.0	15.602409	1
AGGTCCG	95	7.06063E-5	15.578948	5
TGATACT	640	0.0	15.3203125	35
TAAGCCG	85	5.3647166E-4	15.235294	10
ACACTTA	110	1.4520336E-5	15.136364	6
TTGCGAT	135	3.9728184E-7	15.074073	11
ACTCTGT	690	0.0	15.014493	37
>>END_MODULE