FastQCFastQC Report
Wed 25 May 2016
SRR2088567_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088567_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5245453
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT728091.388040270306492No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT526551.0038217862213235No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT510730.9736623319282434No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222350.4238909394479371No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG117370.22375569850687826No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA112000.21351826048198316No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG101740.1939584626914015No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA97420.185722758358525No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG95270.18162397032248692No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA87210.1662582812199442No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA72450.13811962474928285No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT66380.12654769759637538No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC62350.11886485304510401No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA55580.10595843676418414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA184700.021.1442891
GTATTGG16150.020.046441
ATTGGAC17600.018.7102283
TTGGACC22750.018.0527484
TGGACCC22050.017.9546495
GGACCCT22000.017.659096
GACCCTC23350.016.0042847
TAGGAGT5500.014.7999994
TAGTACT7500.014.5533324
CCCTCGC24600.014.5142289
ACCGTTA3700.014.58
ACCCTCG26650.014.3001888
GTCGTAC1854.9112714E-914.01
TCGCATG25900.013.92857212
GTATTAG10900.013.9174311
CTAATAC12950.013.8571423
CCTCGCA25700.013.74902610
GTATCAA286050.013.6138792
TATACCG3400.013.60294155
CTCGCAT26250.013.53142811
Produced by FastQC (version 0.11.5)