##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088567_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5245453
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.663410386100114	31.0	31.0	34.0	30.0	34.0
2	31.822379306420245	31.0	31.0	34.0	30.0	34.0
3	31.956374978481364	31.0	31.0	34.0	30.0	34.0
4	35.63771575114676	37.0	35.0	37.0	33.0	37.0
5	35.54457679822887	37.0	35.0	37.0	33.0	37.0
6	35.586437053196356	37.0	35.0	37.0	33.0	37.0
7	35.45173162356044	37.0	35.0	37.0	33.0	37.0
8	35.48563393857499	37.0	35.0	37.0	33.0	37.0
9	37.09182600625723	39.0	37.0	39.0	33.0	39.0
10	36.9399653375981	39.0	37.0	39.0	33.0	39.0
11	37.006657194335745	39.0	37.0	39.0	33.0	39.0
12	36.86152826076223	39.0	37.0	39.0	32.0	39.0
13	36.91340824138545	39.0	37.0	39.0	33.0	39.0
14	37.94553320752278	40.0	37.0	41.0	33.0	41.0
15	37.950878408404385	40.0	37.0	41.0	33.0	41.0
16	37.84373113246844	40.0	37.0	41.0	32.0	41.0
17	37.86395950931216	40.0	37.0	41.0	32.0	41.0
18	37.829741873580794	40.0	37.0	41.0	32.0	41.0
19	37.88661722829277	40.0	37.0	41.0	32.0	41.0
20	37.785337891694006	40.0	37.0	41.0	32.0	41.0
21	37.64703868283635	40.0	37.0	41.0	32.0	41.0
22	37.528728595985896	39.0	36.0	41.0	32.0	41.0
23	37.36717229188785	39.0	36.0	41.0	31.0	41.0
24	37.52165771002047	39.0	36.0	41.0	32.0	41.0
25	37.47588358908182	39.0	36.0	41.0	32.0	41.0
26	37.27382897149207	39.0	36.0	41.0	31.0	41.0
27	37.23113447017827	39.0	36.0	41.0	31.0	41.0
28	37.08803586649237	39.0	36.0	41.0	31.0	41.0
29	36.877614383352594	39.0	36.0	41.0	30.0	41.0
30	36.74695722180715	39.0	35.0	41.0	30.0	41.0
31	36.53416606725863	39.0	35.0	40.0	30.0	41.0
32	36.38202649037176	38.0	35.0	40.0	30.0	41.0
33	36.22634422613262	38.0	35.0	40.0	30.0	41.0
34	36.04351464020362	38.0	35.0	40.0	29.0	41.0
35	35.853147097114395	38.0	35.0	40.0	28.0	41.0
36	35.79668829365166	38.0	35.0	40.0	27.0	41.0
37	35.667067267593474	38.0	35.0	40.0	27.0	41.0
38	35.52876348334453	38.0	35.0	40.0	26.0	41.0
39	35.4023370336175	38.0	34.0	40.0	26.0	41.0
40	35.239414975217585	38.0	34.0	40.0	25.0	41.0
41	35.12431414407869	38.0	34.0	40.0	24.0	41.0
42	34.86447347826775	38.0	34.0	40.0	23.0	41.0
43	34.498950614942125	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	5.0
13	9.0
14	17.0
15	29.0
16	78.0
17	198.0
18	382.0
19	848.0
20	1830.0
21	3608.0
22	6742.0
23	11580.0
24	18729.0
25	28804.0
26	41924.0
27	59006.0
28	80403.0
29	105340.0
30	134457.0
31	165539.0
32	204552.0
33	252410.0
34	312242.0
35	389348.0
36	510461.0
37	735916.0
38	1006547.0
39	1174446.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.71597762862426	20.951974977185	13.959213818139254	23.372833576051487
2	18.485858132748497	23.60985790931689	35.179668943749945	22.724615014184664
3	19.30994329755695	24.488809641417053	32.96977401189182	23.231473049134173
4	13.377319365934648	16.385810720256192	36.789310665828104	33.44755924798106
5	12.56787545327353	38.81069947628927	34.932426236590054	13.688998833847144
6	31.99643577018038	38.171364799188936	13.960662692049667	15.871536738581016
7	26.10228325370564	33.48713638269183	21.49680875989166	18.91377160371087
8	27.405850362208945	34.42417652012133	19.09335571208054	19.076617405589182
9	25.022033368710005	14.409241680365833	21.458127639309705	39.110597311614455
10	16.203195415152898	28.124987489164425	33.2149959212293	22.456821174453378
11	33.64033573458765	22.77225627605471	23.21366715134041	20.37374083801723
12	21.339091209090995	27.459382440372643	30.80016158756927	20.401364762967088
13	31.38249451477308	20.944520902198533	26.039543200558658	21.63344138246973
14	20.60538908650978	22.50553002762583	28.276528261715434	28.61255262414895
15	22.925417499689733	29.79559630026234	25.202360978165277	22.076625221882647
16	21.872734347252752	28.364909570250653	26.314009485930008	23.448346596566587
17	21.676335675870128	27.136550456176046	27.296250676538325	23.890863191415498
18	22.70475019030768	25.86438197044183	29.587053777814802	21.843814061435683
19	22.844299624836978	25.089768224021835	30.725373004009377	21.34055914713181
20	23.45681107046427	25.086927668592207	30.137320837685515	21.31894042325801
21	22.435659989709183	25.76934728039695	30.572783704286362	21.222209025607512
22	21.52662505983754	26.189234752460845	30.254622432037802	22.02951775566381
23	22.18449007168685	26.159170618819765	30.756866947430474	20.899472362062916
24	22.00444842418758	26.290103066408182	29.089194012414183	22.61625449699006
25	22.072126087108206	26.2384964654149	30.16057907677373	21.528798370703157
26	21.614510700982358	27.078690820411506	30.053743690011135	21.253054788594998
27	20.90135971097253	27.072151823684248	29.797998380692764	22.228490084650456
28	21.293413552652176	26.83261102520602	29.90290829028494	21.971067131856866
29	21.54498381741291	26.858023511029455	29.32356843155396	22.273424240003674
30	21.45563023822728	27.100881468197315	30.50720309570975	20.936285197865658
31	22.039888642601507	26.876134434909627	30.27894826242843	20.805028660060437
32	21.44177061542635	26.800392644829724	29.613114444071847	22.144722295672082
33	21.265465537485515	26.63344805491537	30.262171827676276	21.83891457992284
34	20.815818957866938	26.91300446310357	30.678894654093746	21.59228192493575
35	20.432934962909783	28.573585541610992	29.769878788352504	21.223600707126725
36	22.192954545584527	26.878955926208853	29.526868318141446	21.401221210065174
37	21.23723156036285	27.269865920064483	29.699036479785445	21.793866039787222
38	21.032578120516952	26.14477720036763	30.782012535428304	22.040632143687112
39	20.651714923382215	26.02166104624329	31.35578566808243	21.970838362292064
40	19.84722768462514	26.332597013070174	31.274133997578474	22.54604130472621
41	20.658482689674276	25.38856033978381	31.52145296125997	22.431504009281944
42	19.821395787932904	26.924423877213272	30.843780317924878	22.410400016928946
43	19.825075165100138	26.50939775840142	30.641262060683793	23.02426501581465
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1401.0
1	1356.0
2	1311.0
3	3149.0
4	4987.0
5	4987.0
6	7647.5
7	10308.0
8	10899.0
9	11490.0
10	16925.0
11	22360.0
12	22360.0
13	40164.0
14	57968.0
15	97826.5
16	137685.0
17	125998.5
18	114312.0
19	114312.0
20	117100.0
21	119888.0
22	97431.5
23	74975.0
24	84788.5
25	94602.0
26	94602.0
27	105924.0
28	117246.0
29	126481.5
30	135717.0
31	150704.0
32	165691.0
33	165691.0
34	185216.0
35	204741.0
36	222954.5
37	241168.0
38	255372.5
39	269577.0
40	269577.0
41	280297.0
42	291017.0
43	306216.0
44	321415.0
45	334586.0
46	347757.0
47	347757.0
48	422179.5
49	496602.0
50	468888.5
51	441175.0
52	404692.5
53	368210.0
54	368210.0
55	334491.0
56	300772.0
57	265984.0
58	231196.0
59	209449.5
60	187703.0
61	187703.0
62	165214.5
63	142726.0
64	124305.0
65	105884.0
66	90878.5
67	75873.0
68	75873.0
69	65114.0
70	54355.0
71	46115.5
72	37876.0
73	31328.0
74	24780.0
75	24780.0
76	19703.0
77	14626.0
78	11388.0
79	8150.0
80	6468.0
81	4786.0
82	4786.0
83	3632.0
84	2478.0
85	1839.0
86	1200.0
87	916.5
88	633.0
89	633.0
90	588.5
91	544.0
92	347.0
93	150.0
94	110.5
95	71.0
96	71.0
97	50.0
98	29.0
99	23.5
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5245453.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.9373357335468
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.81321461613139	32.122801132787245
2	12.862143759052064	11.045323696711245
3	4.869810066398645	6.272900093386935
4	2.301688998095109	3.9531357306168267
5	1.2613591725266209	2.7079701135682153
6	0.8199355486188263	2.112350875854986
7	0.5323371587201036	1.5999997515185236
8	0.3832428813507352	1.3164342611238689
9	0.27675390833189273	1.0694767929855944
>10	1.6151175019061608	12.984090891090217
>50	0.14632038238703815	4.381239297355354
>100	0.10417314598703703	8.58233757634782
>500	0.008511047406705415	2.464148288038431
>1k	0.00476796694544297	3.924363867478918
>5k	3.119230711971097E-4	1.0267950763354567
>10k+	3.119230711971097E-4	4.43663255479996
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	72809	1.388040270306492	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	52655	1.0038217862213235	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	51073	0.9736623319282434	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22235	0.4238909394479371	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	11737	0.22375569850687826	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	11200	0.21351826048198316	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10174	0.1939584626914015	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	9742	0.185722758358525	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	9527	0.18162397032248692	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8721	0.1662582812199442	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	7245	0.13811962474928285	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6638	0.12654769759637538	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	6235	0.11886485304510401	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5558	0.10595843676418414	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.906413040017707E-5	0.0	0.0	0.0	0.0
6	1.906413040017707E-5	0.0	0.0	0.0	0.0
7	3.812826080035414E-5	0.0	0.0	0.0	0.0
8	3.812826080035414E-5	0.0	0.0	1.906413040017707E-5	0.0
9	3.812826080035414E-5	0.0	0.0	5.71923912005312E-5	0.0
10	3.812826080035414E-5	0.0	0.0	9.532065200088534E-5	0.0
11	3.812826080035414E-5	0.0	0.0	1.906413040017707E-4	0.0
12	3.812826080035414E-5	0.0	0.0	2.0970543440194773E-4	0.0
13	3.812826080035414E-5	0.0	0.0	2.478336952023019E-4	0.0
14	7.625652160070827E-5	0.0	0.0	2.6689782560247895E-4	0.0
15	7.625652160070827E-5	0.0	0.0	3.4315434720318724E-4	0.0
16	7.625652160070827E-5	0.0	0.0	6.863086944063745E-4	0.0
17	7.625652160070827E-5	0.0	0.0	9.913347808092076E-4	0.0
18	9.532065200088534E-5	0.0	0.0	0.0011819760848109783	0.0
19	9.532065200088534E-5	0.0	0.0	0.0015632586928145196	0.0
20	9.532065200088534E-5	0.0	0.0	0.002783363038425852	0.0
21	9.532065200088534E-5	0.0	0.0	0.005757367380853475	0.0
22	9.532065200088534E-5	0.0	0.0	0.009017333679283753	0.0
23	9.532065200088534E-5	0.0	0.0	0.011743504326509073	0.0
24	9.532065200088534E-5	0.0	0.0	0.01591854888414785	0.0
25	9.532065200088534E-5	0.0	0.0	0.018759104313774234	0.0
26	9.532065200088534E-5	0.0	0.0	0.02478336952023019	0.0
27	9.532065200088534E-5	0.0	0.0	0.06439863249179814	0.0
28	9.532065200088534E-5	0.0	0.0	0.16418029100632492	0.0
29	9.532065200088534E-5	0.0	0.0	0.30796196248446034	0.0
30	9.532065200088534E-5	0.0	0.0	0.4971925208366179	0.0
31	9.532065200088534E-5	0.0	0.0	0.7695808159943479	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18470	0.0	21.144289	1
GTATTGG	1615	0.0	20.04644	1
ATTGGAC	1760	0.0	18.710228	3
TTGGACC	2275	0.0	18.052748	4
TGGACCC	2205	0.0	17.954649	5
GGACCCT	2200	0.0	17.65909	6
GACCCTC	2335	0.0	16.004284	7
TAGGAGT	550	0.0	14.799999	4
TAGTACT	750	0.0	14.553332	4
CCCTCGC	2460	0.0	14.514228	9
ACCGTTA	370	0.0	14.5	8
ACCCTCG	2665	0.0	14.300188	8
GTCGTAC	185	4.9112714E-9	14.0	1
TCGCATG	2590	0.0	13.928572	12
GTATTAG	1090	0.0	13.917431	1
CTAATAC	1295	0.0	13.857142	3
CCTCGCA	2570	0.0	13.749026	10
GTATCAA	28605	0.0	13.613879	2
TATACCG	340	0.0	13.6029415	5
CTCGCAT	2625	0.0	13.531428	11
>>END_MODULE