FastQCFastQC Report
Wed 25 May 2016
SRR2088566_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088566_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4303823
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT748201.7384543927573228No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT529521.2303479952590988No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT519281.2065551952299154No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232300.5397526803495404No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG127380.29596942067552495No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA116650.27103809798869516No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG115640.2686913472045667No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA103980.24159915498383647No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG101010.23469831356912216No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA92730.21545960417052468No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA77980.1811877486597381No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC67830.15760406503706123No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT67390.15658171816080727No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA59290.13776124157522276No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52740.12254221421280569No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA51120.11877811889568879No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT45570.10588260716112165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG14800.020.751
ATTGGAC15300.020.434643
GGTATCA219100.019.031951
ACGTTTA2450.018.8775526
TTGGACC22900.018.0960714
GGACCCT22400.017.6741076
TATACCG3850.017.2987025
ACCGTTA3200.016.7656258
TGGACCC24600.016.5447165
GGACCGT4700.016.5319166
TATTGGA19650.015.6284992
TAGGTCG2500.015.5421
GTTATCG1101.4536312E-515.13636411
GACCCTC25700.014.9007797
ATACGAC2006.184564E-1114.819
GACCGTT3900.014.7051297
CCGTTTA3400.014.69117527
CTAATAC7400.014.53
GTATTAG6300.014.3888881
TAATACT7950.013.9622654
Produced by FastQC (version 0.11.5)