FastQCFastQC Report
Wed 25 May 2016
SRR2088565_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088565_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664799
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT147372.216760253851164No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT121151.8223553284526601No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT83321.253311151190059No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43430.653280164380512No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG27400.4121546512554922No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG24870.37409803564686467No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA24430.367479493801886No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG18420.27707622905569956No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA17790.26759968050493455No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA16370.24623984091432147No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14590.21946483072327125No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC10650.16019879692959826No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC10630.15989795411846286No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA10620.15974753271289518No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT10170.15297856946234878No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9360.14079443561136523No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA8690.1307162014383295No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8640.12996409441049098No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT7830.11777996055950746No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT7470.11236478995907033No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA7410.11146226152566414No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6870.10333950562500846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTTA604.3170076E-827.75000230
GGTATCA36500.025.6465761
ATTATAC551.901094E-523.5454543
CGACGGG400.001930335823.12520
TGATACT2750.020.85454635
GATACTG2950.019.44067836
CTGCTTA801.6150007E-518.59
CTTCGTT902.149438E-618.529
GTACCAT500.007031836518.56
TACCCCA500.007031836518.55
ACGTTCG902.149438E-618.522
TACATGG500.007031836518.52
CCCATAG500.007031836518.51
AGCACTA500.007031836518.55
AGGTCGC500.007031836518.535
GTATCAA52950.017.6789422
CTGAGCA852.7203196E-517.4117649
ACACTTA852.7203196E-517.4117646
TAGGAGT852.7203196E-517.4117644
ATACTGT3100.017.30645237
Produced by FastQC (version 0.11.5)