##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088565_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	664799
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.9127465594864	31.0	31.0	33.0	28.0	34.0
2	31.060288899351534	31.0	31.0	34.0	28.0	34.0
3	31.051875830138133	31.0	31.0	34.0	28.0	34.0
4	34.84131895505258	35.0	35.0	37.0	32.0	37.0
5	34.68820951896739	35.0	35.0	37.0	32.0	37.0
6	34.90098510978506	35.0	35.0	37.0	32.0	37.0
7	34.746673806669385	35.0	35.0	37.0	32.0	37.0
8	34.8778623313212	35.0	35.0	37.0	32.0	37.0
9	36.33208834549992	38.0	35.0	39.0	32.0	39.0
10	36.064318688806694	38.0	35.0	39.0	31.0	39.0
11	36.230937471325916	38.0	35.0	39.0	32.0	39.0
12	35.97663955571534	37.0	35.0	39.0	30.0	39.0
13	36.122590437109565	38.0	35.0	39.0	32.0	39.0
14	36.83500727287496	38.0	36.0	40.0	31.0	41.0
15	36.90035484409574	38.0	36.0	40.0	31.0	41.0
16	36.689292553087476	38.0	36.0	40.0	31.0	41.0
17	36.8199275269668	38.0	36.0	40.0	31.0	41.0
18	36.80321119616606	38.0	36.0	40.0	31.0	41.0
19	36.88493364159693	39.0	36.0	40.0	31.0	41.0
20	36.78271026280124	39.0	36.0	40.0	31.0	41.0
21	36.62231892647251	38.0	35.0	40.0	30.0	41.0
22	36.47732622943175	38.0	35.0	40.0	30.0	41.0
23	36.287067218813505	38.0	35.0	40.0	30.0	41.0
24	36.41739533302547	38.0	35.0	40.0	30.0	41.0
25	36.339586852567464	38.0	35.0	40.0	30.0	41.0
26	36.045443810836055	38.0	35.0	40.0	29.0	41.0
27	35.97006463607797	38.0	34.0	40.0	29.0	41.0
28	35.769062528674084	38.0	34.0	40.0	28.0	41.0
29	35.528012226251846	38.0	34.0	40.0	27.0	41.0
30	35.36855350263764	38.0	34.0	40.0	27.0	41.0
31	35.03068897516392	37.0	34.0	40.0	26.0	41.0
32	34.85681988089633	37.0	33.0	40.0	26.0	41.0
33	34.62578463565679	37.0	33.0	40.0	25.0	41.0
34	34.37131373542981	37.0	33.0	40.0	24.0	41.0
35	34.11575980108273	37.0	33.0	40.0	24.0	41.0
36	34.02879667388188	37.0	33.0	40.0	23.0	41.0
37	33.835438982308936	37.0	33.0	40.0	22.0	41.0
38	33.65231596317082	37.0	33.0	40.0	21.0	41.0
39	33.46281357222258	36.0	32.0	40.0	21.0	41.0
40	33.22643987129945	36.0	31.0	40.0	19.0	41.0
41	33.026459125239356	36.0	31.0	40.0	18.0	41.0
42	32.6718511911119	36.0	31.0	39.0	15.0	41.0
43	32.23555540847685	36.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	4.0
15	13.0
16	25.0
17	54.0
18	128.0
19	285.0
20	586.0
21	1074.0
22	1880.0
23	2992.0
24	4569.0
25	6536.0
26	9348.0
27	12311.0
28	15990.0
29	20418.0
30	24928.0
31	29452.0
32	35383.0
33	42818.0
34	51599.0
35	63810.0
36	83544.0
37	105919.0
38	104394.0
39	46736.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.625661290104226	24.624886619865556	16.123068777179267	19.62638331285095
2	22.478523583820074	26.037343618146235	31.56578153697584	19.918351261057854
3	23.303133729142193	24.793208172695806	32.757269490477576	19.14638860768443
4	16.966180755386215	18.878488084368357	32.96891240811132	31.186418752134102
5	11.850950437651079	41.32602485864149	33.5245690802784	13.298455623429037
6	31.81187095648459	38.35444999165161	12.484976662118926	17.34870238974487
7	24.137521265826212	35.28389783979819	20.775753272793732	19.802827621581862
8	28.318333812174806	35.33293521801326	18.016874273276585	18.331856696535343
9	24.503195702761285	14.114642170039366	21.759509265206475	39.622652861992876
10	17.100807913369305	29.42784209964215	31.614818915190906	21.85653107179764
11	33.97432908292582	22.529215597496385	22.588932895506762	20.907522424071033
12	20.343592574597736	29.48214422705209	30.87309096433659	19.30117223401359
13	33.544575127218906	20.802377861579213	26.325099766997244	19.327947244204637
14	19.59434355346503	23.7170934372645	29.293816627281327	27.394746381989144
15	22.727471010034613	30.700407190744873	25.90647699530234	20.665644803918177
16	20.376986126633764	28.700103339505624	27.86902507374409	23.053885460116515
17	21.22145189749082	27.437766903981505	28.013128780277945	23.327652418249727
18	22.214233174237627	25.187161833877607	30.912802215406458	21.685802776478305
19	22.462428493424326	24.473111421647747	33.04051299716155	20.023947087766377
20	23.121725514027546	24.22401357402764	32.68837648672756	19.965884425217244
21	21.87112194813771	24.87533826013577	33.64039356256553	19.613146229160993
22	20.211522580509296	25.41053762114564	33.086842790076396	21.291097008268665
23	21.157673221530114	25.621428431751553	33.825111048602665	19.39578729811567
24	21.123076298249547	25.530423481383096	31.7745664479038	21.571933772463556
25	21.100362666008824	26.05840261492571	32.525770947308885	20.315463771756576
26	20.59088536535103	26.97161096812721	32.46785870616532	19.96964496035644
27	19.910980612185035	26.67482953494214	32.29607746100701	21.11811239186581
28	20.279061791609195	26.86375882033517	32.44890560906379	20.408273778991845
29	20.191817376379927	26.0170367283946	31.7936699664109	21.997475928814573
30	20.58050628836686	26.6102987519536	33.31427995529476	19.494915004384783
31	21.3151644331595	26.50169449713372	32.56503093416205	19.61811013554473
32	20.987095347616346	26.394143192152814	31.87670258228427	20.74205887794657
33	20.56486246218782	26.431297279328035	32.369483106924044	20.634357151560096
34	19.662033185970497	26.980786673866834	33.107450522639176	20.249729617523492
35	19.196930199955176	28.797425988907925	32.09150434943494	19.914139461701957
36	21.61811314397284	26.27907081689353	31.855944428315926	20.246871610817706
37	20.540343773080284	27.59601022263872	31.537351891323546	20.32629411295745
38	20.56486246218782	25.583973501765193	32.87565113665935	20.975512899387635
39	19.63044469080128	25.76372708141859	33.72101943594981	20.884808791830313
40	18.792447040383635	26.173625411590574	32.861060260319285	22.17286728770651
41	20.2799643200426	25.32118730623843	33.08187888369267	21.31696949002631
42	19.491455312056726	27.174078180021326	31.80134145809485	21.53312504982709
43	19.726864811770174	26.89835574361574	31.269902632224177	22.10487681238991
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	279.0
1	226.5
2	174.0
3	295.5
4	417.0
5	417.0
6	838.5
7	1260.0
8	1440.0
9	1620.0
10	2575.0
11	3530.0
12	3530.0
13	7031.0
14	10532.0
15	18821.5
16	27111.0
17	23394.5
18	19678.0
19	19678.0
20	21691.5
21	23705.0
22	17932.0
23	12159.0
24	12784.5
25	13410.0
26	13410.0
27	14370.0
28	15330.0
29	16145.0
30	16960.0
31	17907.5
32	18855.0
33	18855.0
34	20817.5
35	22780.0
36	24878.0
37	26976.0
38	28173.5
39	29371.0
40	29371.0
41	30705.5
42	32040.0
43	33737.0
44	35434.0
45	38427.5
46	41421.0
47	41421.0
48	56509.5
49	71598.0
50	65120.5
51	58643.0
52	52830.5
53	47018.0
54	47018.0
55	41335.5
56	35653.0
57	31074.5
58	26496.0
59	23389.5
60	20283.0
61	20283.0
62	17961.0
63	15639.0
64	13529.0
65	11419.0
66	9979.5
67	8540.0
68	8540.0
69	7185.5
70	5831.0
71	5044.5
72	4258.0
73	3491.5
74	2725.0
75	2725.0
76	2183.0
77	1641.0
78	1299.5
79	958.0
80	733.5
81	509.0
82	509.0
83	382.5
84	256.0
85	191.0
86	126.0
87	106.0
88	86.0
89	86.0
90	68.0
91	50.0
92	34.0
93	18.0
94	11.5
95	5.0
96	5.0
97	4.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	664799.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.267246143808926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.50161295482573	50.40210773875322
2	7.469757701808419	8.704844212917672
3	2.1981024621477774	3.8423213163383103
4	1.0501955623426114	2.4476801332061107
5	0.6091616643997906	1.7747086320477463
6	0.41051276096990125	1.43516688531647
7	0.2952573007676892	1.2042680873711302
8	0.2077043215416366	0.96818870627195
9	0.16506943706265098	0.8656327368134514
>10	0.9790808314617098	10.487628539148721
>50	0.06688268873449184	2.7199430883541513
>100	0.0370706160727136	4.222880825376816
>500	0.0057031717034860905	2.1455019939051354
>1k	0.003110820929174231	3.4646141392707746
>5k	2.5923507743118594E-4	1.2585414399618127
>10k+	5.184701548623719E-4	4.055971524946543
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14737	2.216760253851164	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12115	1.8223553284526601	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8332	1.253311151190059	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4343	0.653280164380512	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2740	0.4121546512554922	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2487	0.37409803564686467	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2443	0.367479493801886	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1842	0.27707622905569956	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1779	0.26759968050493455	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1637	0.24623984091432147	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1459	0.21946483072327125	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1065	0.16019879692959826	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1063	0.15989795411846286	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1062	0.15974753271289518	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	1017	0.15297856946234878	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	936	0.14079443561136523	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	869	0.1307162014383295	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	864	0.12996409441049098	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	783	0.11777996055950746	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	747	0.11236478995907033	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	741	0.11146226152566414	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	687	0.10333950562500846	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.5042140556769792E-4	0.0	0.0	0.0	0.0
7	1.5042140556769792E-4	0.0	0.0	0.0	0.0
8	1.5042140556769792E-4	0.0	0.0	0.0	0.0
9	1.5042140556769792E-4	0.0	0.0	0.0	0.0
10	3.0084281113539583E-4	0.0	0.0	0.0	0.0
11	3.0084281113539583E-4	0.0	0.0	1.5042140556769792E-4	0.0
12	3.0084281113539583E-4	0.0	0.0	3.0084281113539583E-4	0.0
13	3.0084281113539583E-4	0.0	0.0	3.0084281113539583E-4	0.0
14	4.512642167030937E-4	0.0	0.0	6.016856222707917E-4	0.0
15	4.512642167030937E-4	0.0	0.0	0.0010529498389738854	0.0
16	4.512642167030937E-4	0.0	0.0	0.001805056866812375	0.0
17	4.512642167030937E-4	0.0	0.0	0.0022563210835154688	0.0
18	4.512642167030937E-4	0.0	0.0	0.0027075853002185624	0.0
19	4.512642167030937E-4	0.0	0.0	0.00361011373362475	0.0
20	4.512642167030937E-4	0.0	0.0	0.0060168562227079165	0.0
21	4.512642167030937E-4	0.0	0.0	0.01083034120087425	0.0
22	4.512642167030937E-4	0.0	0.0	0.016245511801311372	0.0
23	4.512642167030937E-4	0.0	0.0	0.021359839590613102	0.0
24	4.512642167030937E-4	0.0	0.0	0.02797838143559181	0.0
25	4.512642167030937E-4	0.0	0.0	0.03173891657478426	0.0
26	4.512642167030937E-4	0.0	0.0	0.03670282295851829	0.0
27	4.512642167030937E-4	0.0	0.0	0.07114932483352111	0.0
28	4.512642167030937E-4	0.0	0.0	0.16832155283025396	0.0
29	4.512642167030937E-4	0.0	0.0	0.3167874801255718	0.0
30	4.512642167030937E-4	0.0	0.0	0.5162462639083392	0.0
31	4.512642167030937E-4	0.0	0.0	0.7874560581468986	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTTA	60	4.3170076E-8	27.750002	30
GGTATCA	3650	0.0	25.646576	1
ATTATAC	55	1.901094E-5	23.545454	3
CGACGGG	40	0.0019303358	23.125	20
TGATACT	275	0.0	20.854546	35
GATACTG	295	0.0	19.440678	36
CTGCTTA	80	1.6150007E-5	18.5	9
CTTCGTT	90	2.149438E-6	18.5	29
GTACCAT	50	0.0070318365	18.5	6
TACCCCA	50	0.0070318365	18.5	5
ACGTTCG	90	2.149438E-6	18.5	22
TACATGG	50	0.0070318365	18.5	2
CCCATAG	50	0.0070318365	18.5	1
AGCACTA	50	0.0070318365	18.5	5
AGGTCGC	50	0.0070318365	18.5	35
GTATCAA	5295	0.0	17.678942	2
CTGAGCA	85	2.7203196E-5	17.411764	9
ACACTTA	85	2.7203196E-5	17.411764	6
TAGGAGT	85	2.7203196E-5	17.411764	4
ATACTGT	310	0.0	17.306452	37
>>END_MODULE