FastQCFastQC Report
Wed 25 May 2016
SRR2088564_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088564_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780248
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT104421.3382924403522982No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74250.9516205104018208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT71050.9106079092801262No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32280.41371461381509467No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG16330.20929243009914794No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA14600.1871199926177318No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA14000.17943012990741405No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG13710.17571336293076048No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA13000.16661369205688448No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG12550.16084629502414616No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA11530.14777352841660601No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9340.11970552952394622No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC8590.11009320113604905No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC358.86683E-426.4285723
GGTATCA23600.022.1843221
ACTAGAC450.00382472420.5555553
GTAATAC555.1410584E-420.1818183
TATACCG555.1410584E-420.1818185
ACGGGTG656.8985E-519.9230775
ATGCGAC500.007032904318.521
TTGGACC3450.017.6956524
TTCGTTA852.7213011E-517.41176430
CCGGTCT852.7213011E-517.4117649
CTTATTG650.001579319417.0769233
GGGTAAG1107.799699E-716.8181821
CTAACCT1005.87292E-616.6500024
TGGACCC3950.016.3924055
GTATTGG2600.016.3653851
GGACCCT3750.016.2799996
AGTACAC803.3808238E-416.18753
ACGTTCG957.055029E-515.57894822
TACTCCG957.055029E-515.5789485
TAGATGT1557.194103E-915.5161294
Produced by FastQC (version 0.11.5)