##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088564_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	780248
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.815717566722377	31.0	31.0	34.0	30.0	34.0
2	31.98122520019276	31.0	31.0	34.0	30.0	34.0
3	32.118954486265906	33.0	31.0	34.0	30.0	34.0
4	35.770211522490285	37.0	35.0	37.0	35.0	37.0
5	35.67879827952138	37.0	35.0	37.0	33.0	37.0
6	35.72405824814674	37.0	35.0	37.0	33.0	37.0
7	35.58269550194297	37.0	35.0	37.0	33.0	37.0
8	35.60874619351796	37.0	35.0	37.0	33.0	37.0
9	37.24577826537204	39.0	37.0	39.0	34.0	39.0
10	37.10252893951667	39.0	37.0	39.0	33.0	39.0
11	37.16570500661328	39.0	37.0	39.0	33.0	39.0
12	37.016806194953396	39.0	37.0	39.0	33.0	39.0
13	37.06119720909249	39.0	37.0	39.0	33.0	39.0
14	38.1397914509233	40.0	37.0	41.0	33.0	41.0
15	38.13832012385806	40.0	37.0	41.0	33.0	41.0
16	38.045152310547415	40.0	37.0	41.0	33.0	41.0
17	38.0451279593155	40.0	37.0	41.0	33.0	41.0
18	37.99898493812224	40.0	37.0	41.0	33.0	41.0
19	38.051639991387354	40.0	37.0	41.0	33.0	41.0
20	37.94725651331372	40.0	37.0	41.0	33.0	41.0
21	37.801718171658244	40.0	37.0	41.0	32.0	41.0
22	37.68696491371974	40.0	37.0	41.0	32.0	41.0
23	37.5145338405225	39.0	36.0	41.0	32.0	41.0
24	37.682645774164115	40.0	37.0	41.0	32.0	41.0
25	37.62932682941834	40.0	37.0	41.0	32.0	41.0
26	37.44010237770555	39.0	36.0	41.0	32.0	41.0
27	37.40177353866976	39.0	36.0	41.0	32.0	41.0
28	37.26129128174632	39.0	36.0	41.0	31.0	41.0
29	37.05098122648184	39.0	36.0	41.0	31.0	41.0
30	36.93731864740442	39.0	36.0	41.0	31.0	41.0
31	36.699531431032185	39.0	35.0	41.0	30.0	41.0
32	36.553299720089	39.0	35.0	40.0	30.0	41.0
33	36.38637715187991	39.0	35.0	40.0	30.0	41.0
34	36.19757820590377	38.0	35.0	40.0	30.0	41.0
35	36.00728870820559	38.0	35.0	40.0	29.0	41.0
36	35.955775599552965	38.0	35.0	40.0	29.0	41.0
37	35.83002711958249	38.0	35.0	40.0	27.0	41.0
38	35.690281807835454	38.0	35.0	40.0	27.0	41.0
39	35.582341768258296	38.0	35.0	40.0	26.0	41.0
40	35.421057151059664	38.0	35.0	40.0	25.0	41.0
41	35.30907352534066	38.0	34.0	40.0	25.0	41.0
42	35.05518501809681	38.0	34.0	40.0	24.0	41.0
43	34.67123658118957	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	6.0
16	8.0
17	19.0
18	49.0
19	101.0
20	229.0
21	457.0
22	858.0
23	1538.0
24	2547.0
25	3885.0
26	5574.0
27	7972.0
28	10992.0
29	14735.0
30	18460.0
31	23060.0
32	28294.0
33	35520.0
34	44893.0
35	56021.0
36	74945.0
37	109247.0
38	150857.0
39	189979.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.5439706349776	20.854651341624713	13.961586572474394	23.639791450923294
2	18.415939547425946	23.072535911658857	35.61649629348617	22.89502824742902
3	19.167110969845485	24.26792506997775	32.63821246578011	23.926751494396655
4	13.47674072858886	16.140509171442925	36.53735735304672	33.84539274692149
5	12.57843659964524	38.55927858834627	34.98887533194574	13.873409480062747
6	32.31985214957296	37.9041022854272	14.098081635582533	15.677963929417313
7	26.147840173893428	33.32427638391896	21.543406711712173	18.98447673047544
8	27.100870492458807	34.502747844275156	19.301427238519032	19.094954424747005
9	24.957193097579232	14.479242497257284	21.44331033209954	39.12025407306395
10	16.214331853461978	27.948037034378814	33.27403594754488	22.563595164614327
11	33.74709066860793	22.627549189488473	23.16212281223406	20.46323732966954
12	21.259394448944438	27.336436620151545	30.873260809383684	20.530908121520337
13	31.308378874409158	21.13968891942049	25.859342157878007	21.692590048292338
14	20.620623186474045	22.48695286626816	28.032497359813803	28.85992658744399
15	22.85504096133537	29.82602967261691	25.050624929509592	22.268304436538124
16	22.040940829069733	28.46492397289067	26.09529277870626	23.39884241933334
17	21.774102593021706	27.187509612328387	27.120607806748627	23.917779987901284
18	22.79506003219489	26.06222636905189	29.338876869918284	21.803836728834934
19	22.93270857470958	25.2832432764967	30.391875403717794	21.392172745075925
20	23.436907239749413	25.216725964052454	29.881140355374185	21.465226440823944
21	22.589356204693892	25.82332796751802	30.227312341716996	21.360003486071093
22	21.601080682039555	26.357388932749586	29.876782766505013	22.164747618705846
23	22.2845813126083	26.23230049932842	30.311131845259453	21.171986342803827
24	22.20524756231352	26.27151879915104	28.876716121028185	22.646517517507252
25	22.138089427976745	26.328423783207388	29.83205339840666	21.701433390409203
26	21.70912325311952	27.232110816048234	29.69773712973311	21.36102880109914
27	21.09649752386421	27.19237985871159	29.424362510381314	22.286760107042888
28	21.476248577375397	26.803272805569513	29.579697737129734	22.140780879925355
29	21.556992135833735	26.930411869046765	29.003727020126934	22.508868974992566
30	21.5731408475254	27.090232849042867	30.242435738380617	21.094190565051115
31	22.076698690672707	26.74790579405522	30.091842593636898	21.083552921635174
32	21.545841834903772	26.674595769550198	29.446406783484225	22.333155612061805
33	21.41767745639848	26.48568147563339	30.08581886784715	22.010822200120987
34	20.85939342362941	26.670238180681018	30.580789697634597	21.889578698054976
35	20.412740564538453	28.12490387671612	29.816673672986028	21.645681885759398
36	22.17782038531339	26.55565922629728	29.554064861428675	21.712455526960657
37	21.29669028308948	26.871712583691338	29.695302006541507	22.13629512667767
38	21.072787113840725	25.850370651382637	30.80469286688335	22.272149367893284
39	20.603961817268356	25.69054967138653	31.486527360531525	22.218961150813588
40	19.826773026012244	25.979304016158967	31.443592293732248	22.750330664096545
41	20.59652828331505	25.163537746972757	31.56560478206929	22.674329187642904
42	19.731034235268787	26.570782622960902	31.07306907547344	22.62511406629687
43	19.68592037403492	26.376998082660897	30.73151100675683	23.205570536547356
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	258.0
1	238.0
2	218.0
3	520.5
4	823.0
5	823.0
6	1164.0
7	1505.0
8	1600.0
9	1695.0
10	2494.0
11	3293.0
12	3293.0
13	5993.5
14	8694.0
15	14290.5
16	19887.0
17	18296.5
18	16706.0
19	16706.0
20	16777.0
21	16848.0
22	13852.5
23	10857.0
24	12557.5
25	14258.0
26	14258.0
27	15858.5
28	17459.0
29	18995.5
30	20532.0
31	22397.5
32	24263.0
33	24263.0
34	27280.5
35	30298.0
36	32811.0
37	35324.0
38	37300.5
39	39277.0
40	39277.0
41	41025.0
42	42773.0
43	44882.5
44	46992.0
45	49157.5
46	51323.0
47	51323.0
48	62391.5
49	73460.0
50	68924.5
51	64389.0
52	59587.5
53	54786.0
54	54786.0
55	50234.5
56	45683.0
57	40726.0
58	35769.0
59	32472.5
60	29176.0
61	29176.0
62	25746.5
63	22317.0
64	19643.0
65	16969.0
66	14521.5
67	12074.0
68	12074.0
69	10180.5
70	8287.0
71	6986.0
72	5685.0
73	4633.0
74	3581.0
75	3581.0
76	2841.0
77	2101.0
78	1678.5
79	1256.0
80	976.0
81	696.0
82	696.0
83	529.0
84	362.0
85	276.0
86	190.0
87	141.0
88	92.0
89	92.0
90	73.0
91	54.0
92	37.0
93	20.0
94	17.0
95	14.0
96	14.0
97	7.5
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	780248.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.889870102292264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.84559703651003	53.988184464798415
2	8.237662082100377	10.361309965797423
3	2.3052941333755603	4.349389457866963
4	1.0426155725795554	2.6227983170462132
5	0.6004325100906495	1.888056128239712
6	0.38775176539891615	1.4631394894723406
7	0.256735926485464	1.1302262347083742
8	0.19188398307077897	0.9654047016025392
9	0.1579433067165513	0.8939730638637374
>10	0.8764752056568484	9.919842926771718
>50	0.06105681710572316	2.623419367884357
>100	0.031650879562835896	3.8595534946082974
>500	0.002654589896150969	1.0839985037010371
>1k	0.0016335937822467503	1.6437840122465672
>5k	4.0839844556168756E-4	1.8659516951517672
>10k+	2.0419922278084378E-4	1.3409681762405197
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10442	1.3382924403522982	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7425	0.9516205104018208	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7105	0.9106079092801262	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3228	0.41371461381509467	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1633	0.20929243009914794	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1460	0.1871199926177318	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1400	0.17943012990741405	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1371	0.17571336293076048	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1300	0.16661369205688448	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1255	0.16084629502414616	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1153	0.14777352841660601	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	934	0.11970552952394622	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	859	0.11009320113604905	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	1.2816437850529577E-4	0.0	0.0	3.8449313551588727E-4	0.0
10	1.2816437850529577E-4	0.0	0.0	5.126575140211831E-4	0.0
11	1.2816437850529577E-4	0.0	0.0	0.0010253150280423661	0.0
12	1.2816437850529577E-4	0.0	0.0	0.0014098081635582533	0.0
13	1.2816437850529577E-4	0.0	0.0	0.001537972542063549	0.0
14	1.2816437850529577E-4	0.0	0.0	0.001537972542063549	0.0
15	1.2816437850529577E-4	0.0	0.0	0.0017943012990741406	0.0
16	1.2816437850529577E-4	0.0	0.0	0.002563287570105915	0.0
17	1.2816437850529577E-4	0.0	0.0	0.0033322738411376896	0.0
18	1.2816437850529577E-4	0.0	0.0	0.0041012601121694645	0.0
19	3.8449313551588727E-4	0.0	0.0	0.004870246383201239	0.0
20	3.8449313551588727E-4	0.0	0.0	0.007818027088823041	0.0
21	3.8449313551588727E-4	0.0	0.0	0.01179112282248721	0.0
22	3.8449313551588727E-4	0.0	0.0	0.019737314289815545	0.0
23	3.8449313551588727E-4	0.0	0.0	0.024351231916006192	0.0
24	3.8449313551588727E-4	0.0	0.0	0.032041094626323936	0.0
25	3.8449313551588727E-4	0.0	0.0	0.03575786160297752	0.0
26	3.8449313551588727E-4	0.0	0.0	0.04242240928525289	0.0
27	3.8449313551588727E-4	0.0	0.0	0.09509796885092944	0.0
28	3.8449313551588727E-4	0.0	0.0	0.2196737447580769	0.0
29	3.8449313551588727E-4	0.0	0.0	0.3984630527729645	0.0
30	3.8449313551588727E-4	0.0	0.0	0.6327475366806451	0.0
31	3.8449313551588727E-4	0.0	0.0	1.0033989193179604	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAC	35	8.86683E-4	26.428572	3
GGTATCA	2360	0.0	22.184322	1
ACTAGAC	45	0.003824724	20.555555	3
GTAATAC	55	5.1410584E-4	20.181818	3
TATACCG	55	5.1410584E-4	20.181818	5
ACGGGTG	65	6.8985E-5	19.923077	5
ATGCGAC	50	0.0070329043	18.5	21
TTGGACC	345	0.0	17.695652	4
TTCGTTA	85	2.7213011E-5	17.411764	30
CCGGTCT	85	2.7213011E-5	17.411764	9
CTTATTG	65	0.0015793194	17.076923	3
GGGTAAG	110	7.799699E-7	16.818182	1
CTAACCT	100	5.87292E-6	16.650002	4
TGGACCC	395	0.0	16.392405	5
GTATTGG	260	0.0	16.365385	1
GGACCCT	375	0.0	16.279999	6
AGTACAC	80	3.3808238E-4	16.1875	3
ACGTTCG	95	7.055029E-5	15.578948	22
TACTCCG	95	7.055029E-5	15.578948	5
TAGATGT	155	7.194103E-9	15.516129	4
>>END_MODULE