FastQCFastQC Report
Wed 25 May 2016
SRR2088563_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088563_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2014882
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT367901.825913378550208No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT267091.3255863122505438No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT258801.284442463628143No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109500.5434561428411192No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG68140.3381835760109029No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA58660.2911336743293156No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG58160.28865213943049767No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA57910.28741137198108874No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA55860.27723707889593535No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG55590.2758970500505737No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA40170.19936651377102976No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32820.1628879507584067No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC32630.16194496749685588No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA30320.1504802762643172No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA28420.1410504436488092No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25920.12864276915471973No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT23400.11613583326467752No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA22840.11335651417800148No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA21820.10829418298441298No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT20370.1010977317778411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG8050.022.521741
ATTGGAC8750.021.3542863
GGTATCA93400.020.8372571
TTGGACC12050.020.265564
GGACCCT12000.018.96256
ACCGTCC801.6176558E-518.58
TGGACCC13600.017.9558835
TATTGGA10250.017.6878052
CGCGATA852.7247634E-517.41176414
ACGTTTA1301.3948011E-817.07692326
GTCTATA904.4486304E-516.4444451
GACCCTC13750.016.2799997
TTAGAAC1659.749783E-1015.6969713
GGCGCTA855.36664E-415.23529410
TATACCG855.36664E-415.2352945
TAGAACA1954.1836756E-1115.1794874
TCTATAC1508.110146E-814.83
GCGCTAG908.277827E-414.38888811
ACGGGCG1551.2114288E-714.322585
TGCGCGA1051.6566081E-414.09523812
Produced by FastQC (version 0.11.5)