##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088563_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2014882
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.768872321059	31.0	31.0	34.0	30.0	34.0
2	31.918008101715138	31.0	31.0	34.0	30.0	34.0
3	32.044367858762946	33.0	31.0	34.0	30.0	34.0
4	35.69539853946782	37.0	35.0	37.0	35.0	37.0
5	35.62236001909789	37.0	35.0	37.0	33.0	37.0
6	35.688229385145135	37.0	35.0	37.0	33.0	37.0
7	35.536344560128086	37.0	35.0	37.0	33.0	37.0
8	35.58379895199818	37.0	35.0	37.0	33.0	37.0
9	37.194461015583045	39.0	37.0	39.0	33.0	39.0
10	37.06241308424017	39.0	37.0	39.0	33.0	39.0
11	37.12405490743379	39.0	37.0	39.0	33.0	39.0
12	36.97498612821992	39.0	37.0	39.0	33.0	39.0
13	37.02834558053524	39.0	37.0	39.0	33.0	39.0
14	38.035662138030915	40.0	37.0	41.0	33.0	41.0
15	38.03736595989244	40.0	37.0	41.0	33.0	41.0
16	37.93086493402591	40.0	37.0	41.0	33.0	41.0
17	37.98893036912335	40.0	37.0	41.0	33.0	41.0
18	37.96458849699387	40.0	37.0	41.0	33.0	41.0
19	38.04015719034663	40.0	37.0	41.0	33.0	41.0
20	37.942771834777425	40.0	37.0	41.0	33.0	41.0
21	37.81040080759072	40.0	37.0	41.0	32.0	41.0
22	37.69404163618515	40.0	37.0	41.0	32.0	41.0
23	37.499339415409935	39.0	36.0	41.0	32.0	41.0
24	37.652450118667	40.0	37.0	41.0	32.0	41.0
25	37.59067032213301	40.0	37.0	41.0	32.0	41.0
26	37.38820387496637	39.0	36.0	41.0	32.0	41.0
27	37.34729279431748	39.0	36.0	41.0	32.0	41.0
28	37.195792607209754	39.0	36.0	41.0	31.0	41.0
29	36.976543043215436	39.0	35.0	41.0	31.0	41.0
30	36.80823393131707	39.0	35.0	41.0	30.0	41.0
31	36.524283307905876	39.0	35.0	40.0	30.0	41.0
32	36.361369549184516	38.0	35.0	40.0	30.0	41.0
33	36.180593205954494	38.0	35.0	40.0	30.0	41.0
34	35.97325352055356	38.0	35.0	40.0	29.0	41.0
35	35.74882598583937	38.0	35.0	40.0	28.0	41.0
36	35.67261804909667	38.0	35.0	40.0	27.0	41.0
37	35.505210230673555	38.0	35.0	40.0	26.0	41.0
38	35.3591381530035	38.0	35.0	40.0	25.0	41.0
39	35.21717897127474	38.0	34.0	40.0	25.0	41.0
40	35.01548825191748	38.0	34.0	40.0	24.0	41.0
41	34.85664421043019	38.0	34.0	40.0	23.0	41.0
42	34.53250909978848	38.0	33.0	40.0	22.0	41.0
43	34.13120817993312	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	4.0
13	4.0
14	8.0
15	5.0
16	27.0
17	45.0
18	160.0
19	303.0
20	634.0
21	1296.0
22	2423.0
23	4238.0
24	6881.0
25	10672.0
26	16024.0
27	22583.0
28	31202.0
29	40706.0
30	51377.0
31	62673.0
32	76778.0
33	94945.0
34	117929.0
35	148112.0
36	196065.0
37	291763.0
38	379430.0
39	458593.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.05864164750094	20.803401886562092	14.67495366974344	22.463002796193525
2	18.25352551663075	23.90546940217839	35.83346320032637	22.007541880864487
3	19.22191969554545	24.675489681281583	33.59938696161859	22.503203661554373
4	13.073122892556487	16.16049972157178	36.60611390642231	34.16026347944941
5	12.001794646038826	39.0828346275365	34.93559424323608	13.979776483188594
6	31.517627335000263	38.312764717735334	13.340632354649056	16.82897559261535
7	25.05764605569954	34.13356216393814	21.41897143356286	19.389820346799468
8	27.50017122590802	34.362508573703074	18.94756119713214	19.189759003256768
9	24.458851684614782	14.465760277773091	21.89031417224433	39.1850738653678
10	16.327904065846038	28.16204621412073	32.80881957355319	22.701230146480043
11	32.789910277624195	22.847243659926487	23.670567308656288	20.692278753793026
12	21.3483469503425	28.08258746666058	30.870443033388558	19.698622549608363
13	31.977852797335032	21.205807585754403	26.41648493559424	20.399854681316327
14	20.381441692367098	22.80957396016243	28.944573429113962	27.86441091835651
15	22.199711943428944	30.07664964995469	26.123763078929684	21.599875327686682
16	21.065253449085354	28.557503615596346	26.912245977680083	23.464996957638213
17	21.309734267316895	26.97929705064614	27.54811448015318	24.162854201883782
18	22.846201415268983	25.41851086068564	30.545361961643415	21.189925762401966
19	22.64375779822342	24.52426494454762	31.992394591842103	20.839582665386857
20	23.423158279244145	24.423663519749546	31.382383683014687	20.770794517991625
21	22.01731912836583	25.33359273644809	32.16322345427673	20.485864680909355
22	20.55430541341875	25.70378811265374	31.745382608013767	21.996523865913737
23	21.59024697227927	25.890895844024612	32.513417659197906	20.005439524498208
24	21.48120832882521	25.891541043098304	30.367584801492097	22.259665826584385
25	21.45410996773012	25.959535099325915	31.46382765839389	21.122527274550073
26	21.030214176314047	27.012202203404467	31.45539043973791	20.502193180543575
27	19.879327920940284	27.136527101835245	31.135768744770164	21.84837623245431
28	20.58304158754706	26.557336856451148	31.11824910838451	21.74137244761728
29	20.78712301762585	26.40809734763624	30.415428794341302	22.38935084039661
30	20.761860992355878	26.800130230951492	32.129325687558875	20.308683089133755
31	21.605831011443847	26.424574739364388	31.94723065668362	20.022363592508146
32	20.932143917112764	26.450581225104003	30.83123478198723	21.786040075796002
33	20.659721015920535	26.221386661849177	31.690292533260013	21.428599788970274
34	19.782299906396503	26.81268679753951	32.29429812763229	21.1107151684317
35	19.39791015056961	29.01033410393264	30.951837378069786	20.639918367427967
36	21.71437334791814	26.58051439240611	30.736787563738222	20.96832469593753
37	20.698333698946143	27.29971283678151	30.770189023476313	21.231764440796034
38	20.399457635732514	25.76845691211694	32.15339657607741	21.678688876073142
39	19.816445826604237	25.54114831538522	32.96818374475527	21.67422211325527
40	18.808347089308455	25.951594187649697	32.76593865050162	22.474120072540227
41	20.11393223027453	25.104894480173034	32.776112943586774	22.005060345965667
42	19.0492544972857	27.060741026025347	31.999491781652722	21.890512695036236
43	19.215120289922684	26.996022595864172	31.21363930989507	22.57521780431807
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	567.0
1	534.0
2	501.0
3	1372.5
4	2244.0
5	2244.0
6	3422.0
7	4600.0
8	4956.0
9	5312.0
10	8039.0
11	10766.0
12	10766.0
13	19618.0
14	28470.0
15	48262.0
16	68054.0
17	61612.5
18	55171.0
19	55171.0
20	54621.5
21	54072.0
22	41573.5
23	29075.0
24	32016.5
25	34958.0
26	34958.0
27	38728.0
28	42498.0
29	45642.5
30	48787.0
31	52975.0
32	57163.0
33	57163.0
34	64324.5
35	71486.0
36	78420.0
37	85354.0
38	91014.0
39	96674.0
40	96674.0
41	101010.5
42	105347.0
43	111588.5
44	117830.0
45	126628.0
46	135426.0
47	135426.0
48	178205.0
49	220984.0
50	202824.5
51	184665.0
52	167336.0
53	150007.0
54	150007.0
55	131930.5
56	113854.0
57	96419.5
58	78985.0
59	70378.5
60	61772.0
61	61772.0
62	54303.0
63	46834.0
64	40477.5
65	34121.0
66	28880.5
67	23640.0
68	23640.0
69	20250.0
70	16860.0
71	14391.5
72	11923.0
73	9703.0
74	7483.0
75	7483.0
76	5933.5
77	4384.0
78	3356.0
79	2328.0
80	1822.0
81	1316.0
82	1316.0
83	994.0
84	672.0
85	518.0
86	364.0
87	265.0
88	166.0
89	166.0
90	129.0
91	92.0
92	72.5
93	53.0
94	34.0
95	15.0
96	15.0
97	10.5
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2014882.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.31906958801957
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.8448293168525	37.78183034687689
2	10.927701599108346	10.341773448106412
3	3.4631488876481384	4.916189496248846
4	1.6004959000067054	3.0293590747102925
5	0.9135022764338527	2.161303889369389
6	0.5893474409651606	1.6732423542331014
7	0.4091868448776559	1.355363855108758
8	0.3074500385588636	1.1638599815524933
9	0.23855882680662324	1.0159543553849024
>10	1.4958135379620299	13.432145091900624
>50	0.1268168864376115	4.13073249442248
>100	0.07191588933462828	6.427888606419685
>500	0.00514388033071988	1.6989573466260184
>1k	0.005038903181113353	4.1275791709021625
>5k	6.298628976391691E-4	1.7600566255088328
>10k+	4.199085984261127E-4	4.983763862629139
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	36790	1.825913378550208	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	26709	1.3255863122505438	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	25880	1.284442463628143	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10950	0.5434561428411192	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6814	0.3381835760109029	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5866	0.2911336743293156	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5816	0.28865213943049767	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5791	0.28741137198108874	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5586	0.27723707889593535	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5559	0.2758970500505737	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4017	0.19936651377102976	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3282	0.1628879507584067	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3263	0.16194496749685588	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3032	0.1504802762643172	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2842	0.1410504436488092	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2592	0.12864276915471973	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2340	0.11613583326467752	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2284	0.11335651417800148	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2182	0.10829418298441298	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	2037	0.1010977317778411	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.926139595271584E-5	0.0
10	0.0	0.0	0.0	2.4815348988178963E-4	0.0
11	0.0	0.0	0.0	5.459376777399371E-4	0.0
12	0.0	0.0	0.0	0.001141506053456232	0.0
13	0.0	0.0	0.0	0.001290398147385306	0.0
14	0.0	0.0	0.0	0.0014889209392907376	0.0
15	0.0	0.0	0.0	0.0022333814089361063	0.0
16	0.0	0.0	0.0	0.003771933046203202	0.0
17	0.0	0.0	0.0	0.005360115381446655	0.0
18	0.0	0.0	0.0	0.006352729340973814	0.0
19	4.963069797635792E-5	0.0	0.0	0.007990542374193626	0.0
20	4.963069797635792E-5	0.0	0.0	0.014492163809096513	0.0
21	4.963069797635792E-5	0.0	0.0	0.02675094620925692	4.963069797635792E-5
22	4.963069797635792E-5	0.0	0.0	0.0429305537495496	4.963069797635792E-5
23	4.963069797635792E-5	0.0	0.0	0.05270780125089211	4.963069797635792E-5
24	4.963069797635792E-5	0.0	0.0	0.06585993621462696	4.963069797635792E-5
25	4.963069797635792E-5	0.0	0.0	0.07285786462929343	4.963069797635792E-5
26	4.963069797635792E-5	0.0	0.0	0.08566258470719378	4.963069797635792E-5
27	4.963069797635792E-5	0.0	0.0	0.13767555618641686	9.926139595271584E-5
28	4.963069797635792E-5	0.0	0.0	0.2780808007615334	9.926139595271584E-5
29	4.963069797635792E-5	0.0	0.0	0.4803755257131683	9.926139595271584E-5
30	4.963069797635792E-5	0.0	0.0	0.7587541106625599	9.926139595271584E-5
31	4.963069797635792E-5	0.0	0.0	1.144384633938861	9.926139595271584E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTGG	805	0.0	22.52174	1
ATTGGAC	875	0.0	21.354286	3
GGTATCA	9340	0.0	20.837257	1
TTGGACC	1205	0.0	20.26556	4
GGACCCT	1200	0.0	18.9625	6
ACCGTCC	80	1.6176558E-5	18.5	8
TGGACCC	1360	0.0	17.955883	5
TATTGGA	1025	0.0	17.687805	2
CGCGATA	85	2.7247634E-5	17.411764	14
ACGTTTA	130	1.3948011E-8	17.076923	26
GTCTATA	90	4.4486304E-5	16.444445	1
GACCCTC	1375	0.0	16.279999	7
TTAGAAC	165	9.749783E-10	15.696971	3
GGCGCTA	85	5.36664E-4	15.235294	10
TATACCG	85	5.36664E-4	15.235294	5
TAGAACA	195	4.1836756E-11	15.179487	4
TCTATAC	150	8.110146E-8	14.8	3
GCGCTAG	90	8.277827E-4	14.388888	11
ACGGGCG	155	1.2114288E-7	14.32258	5
TGCGCGA	105	1.6566081E-4	14.095238	12
>>END_MODULE