FastQCFastQC Report
Wed 25 May 2016
SRR2088562_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088562_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3398669
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT544231.6013033337462401No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT382071.124175375713257No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT379011.1151718510981798No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161450.4750389049360206No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG86210.25365812322412096No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA70240.20666914018399557No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG68770.20234391757479175No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA67970.19999005493032715No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA60060.17671623803318298No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG58230.17133177723397014No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA54170.15938592431331206No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT50180.14764603437404467No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC43460.12787358816054167No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36050.10607093541618792No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT34710.10212821548670964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140400.021.1748581
GTATTGG10400.017.6105771
ATTGGAC9950.016.3618093
TTGGACC12800.016.18754
TCTATAC2201.8189894E-1215.1363643
CCGTTTA1702.4414476E-814.14705927
GGACCCT14250.014.0210536
GACCCTC13900.013.974827
AATCGCG800.00630196513.87500123
GTATCAA215950.013.7325772
TATACAC19500.013.28205237
TCTACAC4050.013.2469143
TATACTG4350.013.1839085
GTAGCAC2254.0745363E-1013.1555563
ATACACA15150.013.06600737
TAACGCG850.00940968113.05882426
GAACCGT1703.7374593E-713.0588246
TATTGGA12650.013.0158112
CGATAGA1153.5803765E-412.86956537
ATAGGCG1451.3737332E-512.7586232
Produced by FastQC (version 0.11.5)