##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088562_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3398669
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.681987860541877	31.0	31.0	34.0	30.0	34.0
2	31.838288459393958	31.0	31.0	34.0	30.0	34.0
3	31.959285237838696	31.0	31.0	34.0	30.0	34.0
4	35.640609014882	37.0	35.0	37.0	33.0	37.0
5	35.54985172136504	37.0	35.0	37.0	33.0	37.0
6	35.60867033535775	37.0	35.0	37.0	33.0	37.0
7	35.4639551542089	37.0	35.0	37.0	33.0	37.0
8	35.50191413167919	37.0	35.0	37.0	33.0	37.0
9	37.098736593648866	39.0	37.0	39.0	33.0	39.0
10	36.94838891342464	39.0	37.0	39.0	33.0	39.0
11	37.01484375206883	39.0	37.0	39.0	33.0	39.0
12	36.87140348177478	39.0	37.0	39.0	32.0	39.0
13	36.925662663825165	39.0	37.0	39.0	33.0	39.0
14	37.94068530945496	40.0	37.0	41.0	33.0	41.0
15	37.94902975252959	40.0	37.0	41.0	33.0	41.0
16	37.84054081171188	40.0	37.0	41.0	32.0	41.0
17	37.87651342334308	40.0	37.0	41.0	33.0	41.0
18	37.846765895707996	40.0	37.0	41.0	32.0	41.0
19	37.911567145844444	40.0	37.0	41.0	32.0	41.0
20	37.81644696791597	40.0	37.0	41.0	32.0	41.0
21	37.67453906220347	40.0	37.0	41.0	32.0	41.0
22	37.55859543839073	39.0	36.0	41.0	32.0	41.0
23	37.390879782644326	39.0	36.0	41.0	32.0	41.0
24	37.5318823339372	39.0	36.0	41.0	32.0	41.0
25	37.46880175739385	39.0	36.0	41.0	32.0	41.0
26	37.26632778890795	39.0	36.0	41.0	31.0	41.0
27	37.22227583798246	39.0	36.0	41.0	31.0	41.0
28	37.0723130143006	39.0	36.0	41.0	31.0	41.0
29	36.85295155250482	39.0	35.0	41.0	30.0	41.0
30	36.71404894092364	39.0	35.0	40.0	30.0	41.0
31	36.449533332019094	39.0	35.0	40.0	30.0	41.0
32	36.28889721240874	38.0	35.0	40.0	30.0	41.0
33	36.12147019906911	38.0	35.0	40.0	30.0	41.0
34	35.927051737018225	38.0	35.0	40.0	29.0	41.0
35	35.722383085849195	38.0	35.0	40.0	28.0	41.0
36	35.64904996632505	38.0	35.0	40.0	27.0	41.0
37	35.50592040590008	38.0	35.0	40.0	26.0	41.0
38	35.35761117072595	38.0	34.0	40.0	25.0	41.0
39	35.218600281463125	38.0	34.0	40.0	25.0	41.0
40	35.03695799738074	38.0	34.0	40.0	24.0	41.0
41	34.889152488812535	38.0	34.0	40.0	24.0	41.0
42	34.60416121722945	38.0	33.0	40.0	22.0	41.0
43	34.21415000990093	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	7.0
14	7.0
15	21.0
16	43.0
17	87.0
18	231.0
19	547.0
20	1158.0
21	2419.0
22	4371.0
23	7657.0
24	12549.0
25	19333.0
26	28106.0
27	39334.0
28	53708.0
29	70333.0
30	88680.0
31	108182.0
32	133268.0
33	164512.0
34	203985.0
35	253169.0
36	334089.0
37	486286.0
38	651210.0
39	735374.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.510838507662854	21.11314752922394	14.556698519332127	22.81931544378108
2	18.554557681256988	23.88879293629359	35.23635281929485	22.320296563154578
3	19.455145529029156	24.510124404583088	33.168719872397105	22.866010193990647
4	13.63775054293313	16.49277996768735	36.10884143174872	33.7606280576308
5	12.529552009919176	38.9489826752767	34.60516455118166	13.916300763622466
6	31.989699497067825	37.95562321602957	13.79772493290756	16.25695235399505
7	25.42565928014761	33.942640486614025	21.380664018767348	19.251036214471018
8	27.474873251852415	34.47764404241778	18.957597812555445	19.089884893174357
9	24.879268913801255	14.558051990352695	21.747896014586885	38.814783081259165
10	16.37747012139164	28.379080163440452	32.74443613073235	22.499013584435552
11	33.404753449070796	22.974788071447968	23.159860521869003	20.46059795761223
12	21.34126624275562	27.861612884337955	30.757040476727802	20.040080396178624
13	31.77149642992595	21.22881045491632	25.90914266732065	21.09055044783708
14	20.424348472887473	22.989999908787823	28.566388783373725	28.019262834950975
15	22.73972546311512	30.162219386471584	25.336977505017405	21.761077645395886
16	21.51918883539409	28.505188354617644	26.611652973561124	23.36396983642714
17	21.611519097623216	27.352737203887756	27.396195392961186	23.639548305527843
18	22.759291946347233	25.812987378294267	29.839416548066318	21.588304127292187
19	22.653897746441327	25.314910042725547	31.24643794379506	20.784754267038068
20	23.29809110566519	25.17023575994014	30.4938492097936	21.03782392460107
21	22.053456809121453	25.91679272091516	31.199978579849937	20.829771890113452
22	21.191178075887944	26.315242820056913	30.68877846003833	21.80480064401682
23	21.98095783967194	26.234152251955106	31.06469032435933	20.720199584013624
24	21.720856017458598	26.418489120299743	29.6838262272672	22.17682863497446
25	21.791354203660315	26.392272975097015	30.562464305879743	21.253908515362927
26	21.192708086606846	27.434681047198183	30.5559029137583	20.816707952436676
27	20.524299365428053	27.249255517380483	30.354824197354908	21.87162091983656
28	20.94546423908889	27.132768739762536	30.23189372074774	21.689873300400834
29	21.079546139974205	26.988653499355188	29.733169072951792	22.198631287718808
30	21.15001490289287	27.26941046627371	31.09537880858654	20.485195822246887
31	21.735979584949284	26.976648799868418	30.774959256108787	20.512412359073508
32	21.187647281921247	27.007131321114237	29.994241863505977	21.81097953345854
33	20.90483068518882	26.72669801030933	30.6982527571823	21.670218547319553
34	20.375476399731777	26.955169803237677	31.309344922968375	21.36000887406217
35	19.95584153678984	28.492094993657812	30.448184274490984	21.103879195061364
36	21.689020025192214	26.80137430270497	30.275381333104225	21.23422433899859
37	20.87976204802527	27.178698484612653	30.346350291834835	21.595189175527242
38	20.499230728264507	26.0198330581766	31.54949775926988	21.93143845428902
39	20.06367786918938	25.861535795336348	32.315356393929505	21.759429941544763
40	19.223525444813838	26.127463427594744	32.19242591732234	22.45658521026908
41	19.976879184174745	25.349188167485565	32.37976396053867	22.29416868780102
42	19.179831869475965	26.928012112977168	31.65139058849214	22.240765429054726
43	19.14019870719979	26.645578018924464	31.319996151434577	22.89422712244117
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1155.0
1	1063.0
2	971.0
3	2295.5
4	3620.0
5	3620.0
6	5515.5
7	7411.0
8	8108.0
9	8805.0
10	12979.5
11	17154.0
12	17154.0
13	30414.5
14	43675.0
15	73956.0
16	104237.0
17	94645.0
18	85053.0
19	85053.0
20	85874.0
21	86695.0
22	68974.5
23	51254.0
24	57518.0
25	63782.0
26	63782.0
27	70501.5
28	77221.0
29	81755.0
30	86289.0
31	93499.5
32	100710.0
33	100710.0
34	112025.5
35	123341.0
36	135088.0
37	146835.0
38	156284.0
39	165733.0
40	165733.0
41	172785.5
42	179838.0
43	190869.0
44	201900.0
45	214930.0
46	227960.0
47	227960.0
48	283752.5
49	339545.0
50	315958.0
51	292371.0
52	265402.5
53	238434.0
54	238434.0
55	215967.0
56	193500.0
57	169478.0
58	145456.0
59	131493.5
60	117531.0
61	117531.0
62	103274.5
63	89018.0
64	77023.0
65	65028.0
66	55537.5
67	46047.0
68	46047.0
69	39393.5
70	32740.0
71	27577.5
72	22415.0
73	18292.5
74	14170.0
75	14170.0
76	11405.0
77	8640.0
78	6671.0
79	4702.0
80	3727.5
81	2753.0
82	2753.0
83	2010.0
84	1267.0
85	1013.5
86	760.0
87	539.5
88	319.0
89	319.0
90	261.0
91	203.0
92	140.0
93	77.0
94	58.0
95	39.0
96	39.0
97	25.0
98	11.0
99	7.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3398669.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.154384067299084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.15251034682998	35.60926594303858
2	12.16403292177743	11.228468945579717
3	4.22426738089661	5.849053773025973
4	1.98506195415406	3.6647724731763898
5	1.0407262082445827	2.401703856211218
6	0.6425690259539835	1.7794426568178257
7	0.45654518029582153	1.475009311681336
8	0.3373346765101533	1.2455579375094168
9	0.24292624218847123	1.0090899973793175
>10	1.5232835665673377	13.20516575653954
>50	0.1380275679251285	4.414851631130318
>100	0.08267170014609175	7.196785891293874
>500	0.0055013863183190825	1.7287053190737374
>1k	0.0037742068921011915	3.338573403311582
>5k	5.117568667255853E-4	1.522976660709263
>10k+	2.5587843336279266E-4	4.330576443521933
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	54423	1.6013033337462401	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	38207	1.124175375713257	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	37901	1.1151718510981798	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16145	0.4750389049360206	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	8621	0.25365812322412096	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7024	0.20666914018399557	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6877	0.20234391757479175	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6797	0.19999005493032715	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	6006	0.17671623803318298	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5823	0.17133177723397014	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5417	0.15938592431331206	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5018	0.14764603437404467	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4346	0.12787358816054167	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3605	0.10607093541618792	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	3471	0.10212821548670964	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.9423283055808023E-5	0.0	0.0	0.0	0.0
3	5.8846566111616046E-5	0.0	0.0	0.0	0.0
4	5.8846566111616046E-5	0.0	0.0	0.0	0.0
5	5.8846566111616046E-5	0.0	0.0	0.0	0.0
6	5.8846566111616046E-5	0.0	0.0	0.0	0.0
7	5.8846566111616046E-5	0.0	0.0	0.0	0.0
8	5.8846566111616046E-5	0.0	0.0	0.0	0.0
9	5.8846566111616046E-5	0.0	0.0	8.826984916742407E-5	0.0
10	5.8846566111616046E-5	0.0	0.0	1.7653969833484814E-4	0.0
11	5.8846566111616046E-5	0.0	0.0	4.4134924583712036E-4	0.0
12	5.8846566111616046E-5	0.0	0.0	7.061587933393926E-4	0.0
13	5.8846566111616046E-5	0.0	0.0	7.061587933393926E-4	0.0
14	8.826984916742407E-5	0.0	0.0	7.944286425068166E-4	0.0
15	8.826984916742407E-5	0.0	0.0	0.001029814906953281	0.0
16	8.826984916742407E-5	0.0	0.0	0.0016477038511252494	0.0
17	8.826984916742407E-5	0.0	0.0	0.00223616951224141	0.0
18	8.826984916742407E-5	0.0	0.0	0.0026775187580785303	0.0
19	1.1769313222323209E-4	0.0	0.0	0.003442524117529539	0.0
20	1.1769313222323209E-4	0.0	0.0	0.005825810045049989	0.0
21	1.1769313222323209E-4	0.0	0.0	0.011622196807044169	0.0
22	1.471164152790401E-4	0.0	0.0	0.017624546550429006	0.0
23	1.471164152790401E-4	0.0	0.0	0.02247938825463733	0.0
24	1.471164152790401E-4	0.0	0.0	0.029717515886366105	0.0
25	1.471164152790401E-4	0.0	0.0	0.03363081253278857	0.0
26	1.471164152790401E-4	0.0	0.0	0.04195760163758224	0.0
27	1.471164152790401E-4	0.0	0.0	0.09418392906164148	0.0
28	1.471164152790401E-4	0.0	0.0	0.23206143346115787	0.0
29	1.471164152790401E-4	0.0	0.0	0.4425556004424085	0.0
30	1.471164152790401E-4	0.0	0.0	0.71881080505339	0.0
31	1.471164152790401E-4	0.0	0.0	1.1329140907808322	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14040	0.0	21.174858	1
GTATTGG	1040	0.0	17.610577	1
ATTGGAC	995	0.0	16.361809	3
TTGGACC	1280	0.0	16.1875	4
TCTATAC	220	1.8189894E-12	15.136364	3
CCGTTTA	170	2.4414476E-8	14.147059	27
GGACCCT	1425	0.0	14.021053	6
GACCCTC	1390	0.0	13.97482	7
AATCGCG	80	0.006301965	13.875001	23
GTATCAA	21595	0.0	13.732577	2
TATACAC	1950	0.0	13.282052	37
TCTACAC	405	0.0	13.246914	3
TATACTG	435	0.0	13.183908	5
GTAGCAC	225	4.0745363E-10	13.155556	3
ATACACA	1515	0.0	13.066007	37
TAACGCG	85	0.009409681	13.058824	26
GAACCGT	170	3.7374593E-7	13.058824	6
TATTGGA	1265	0.0	13.015811	2
CGATAGA	115	3.5803765E-4	12.869565	37
ATAGGCG	145	1.3737332E-5	12.75862	32
>>END_MODULE