FastQCFastQC Report
Wed 25 May 2016
SRR2088561_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088561_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3125445
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT630062.0159049351372365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT451771.4454581667570539No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT439161.405111912063722No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172560.5521133790548226No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG116120.37153109397221834No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA99490.3183226708516707No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA94410.3020689853764824No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG91970.29426209707737616No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA86670.27730451183751437No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG69550.2225283119683757No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA69130.22118450332672626No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT56380.18039031241951145No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC56320.1801983397564187No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT52690.16858399363930576No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT45880.14679509637827573No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA44040.1409079347100973No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA41280.1320771922078296No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG39410.12609404420810477No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA39340.1258700761011632No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG39070.1250061991172457No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT37480.11991892354528715No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA37230.11911903744906725No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36960.11825516046514978No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT35410.11329586666858639No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA34130.1092004498559405No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34090.10907246808054534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133600.025.0497761
GTATTGG9200.022.9239121
ATTGGAC9900.021.8636363
TTGGACC14500.019.9034484
GGACCCT13950.019.0967736
GACCCTC13600.019.0441177
GGGTACG500.00703752418.4999981
TGGACCC16350.017.7645245
GAACCGT752.0686995E-417.2666666
TATTGGA13550.015.974172
GTATCAA218000.015.3685782
ACCCTCG16500.015.0242438
CGACGAG1853.0559022E-1015.00000124
ATACACA12100.014.83057837
TGAACCG750.00410650614.85
CCCTCGC16500.014.687889
ACACCGA1904.5656634E-1014.6052646
TACGTTA1152.2121389E-514.4782619
TATACTG3750.014.3066675
ACTCTGT18500.014.137
Produced by FastQC (version 0.11.5)