##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088561_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3125445
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.690967526224266	31.0	31.0	34.0	30.0	34.0
2	31.843628027368904	31.0	31.0	34.0	30.0	34.0
3	31.950647027863234	31.0	31.0	34.0	30.0	34.0
4	35.63517707078512	37.0	35.0	37.0	33.0	37.0
5	35.55589044120117	37.0	35.0	37.0	33.0	37.0
6	35.62566706500994	37.0	35.0	37.0	33.0	37.0
7	35.4643102022272	37.0	35.0	37.0	33.0	37.0
8	35.52618107181537	37.0	35.0	37.0	33.0	37.0
9	37.107398786412816	39.0	37.0	39.0	33.0	39.0
10	36.972321381435286	39.0	37.0	39.0	33.0	39.0
11	37.03681491755574	39.0	37.0	39.0	33.0	39.0
12	36.8725285519342	39.0	37.0	39.0	32.0	39.0
13	36.93659686860591	39.0	37.0	39.0	33.0	39.0
14	37.90828729988849	40.0	37.0	41.0	33.0	41.0
15	37.918509844198184	40.0	37.0	41.0	33.0	41.0
16	37.81371548691466	40.0	37.0	41.0	32.0	41.0
17	37.882592398842405	40.0	37.0	41.0	33.0	41.0
18	37.855737023047915	40.0	37.0	41.0	32.0	41.0
19	37.94497967489429	40.0	37.0	41.0	33.0	41.0
20	37.848894157471975	40.0	37.0	41.0	32.0	41.0
21	37.71931292983879	40.0	37.0	41.0	32.0	41.0
22	37.61039979906861	39.0	37.0	41.0	32.0	41.0
23	37.42385228343484	39.0	36.0	41.0	32.0	41.0
24	37.561097699687565	39.0	36.0	41.0	32.0	41.0
25	37.48165653210983	39.0	36.0	41.0	32.0	41.0
26	37.279890703563815	39.0	36.0	41.0	31.0	41.0
27	37.23872280587244	39.0	36.0	41.0	31.0	41.0
28	37.08186418254041	39.0	36.0	41.0	31.0	41.0
29	36.85580101393562	39.0	35.0	41.0	30.0	41.0
30	36.6765836544876	39.0	35.0	40.0	30.0	41.0
31	36.363989767857056	38.0	35.0	40.0	30.0	41.0
32	36.20345550793567	38.0	35.0	40.0	30.0	41.0
33	36.024189835367444	38.0	35.0	40.0	30.0	41.0
34	35.82842347249752	38.0	35.0	40.0	29.0	41.0
35	35.61607195135413	38.0	35.0	40.0	27.0	41.0
36	35.51589997584344	38.0	35.0	40.0	27.0	41.0
37	35.35427339146906	38.0	34.0	40.0	26.0	41.0
38	35.19493192169435	38.0	34.0	40.0	25.0	41.0
39	35.03624091929309	38.0	34.0	40.0	24.0	41.0
40	34.81316580518934	38.0	34.0	40.0	23.0	41.0
41	34.631173160941884	38.0	33.0	40.0	23.0	41.0
42	34.3074435800342	38.0	33.0	40.0	21.0	41.0
43	33.898875520125934	38.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	5.0
14	9.0
15	14.0
16	32.0
17	78.0
18	219.0
19	502.0
20	1093.0
21	2178.0
22	4021.0
23	7036.0
24	11652.0
25	17900.0
26	26539.0
27	37023.0
28	50907.0
29	65949.0
30	82643.0
31	101016.0
32	123761.0
33	152778.0
34	189603.0
35	235664.0
36	312960.0
37	458422.0
38	587192.0
39	656245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.90310339807611	21.51437635280736	15.56741520007551	22.01510504904102
2	18.620484443015314	24.212520137132472	35.67360807820966	21.49338734164255
3	19.119261417174194	24.217511426372884	34.49300819563294	22.17021896081998
4	13.302201766468455	16.504497759519044	36.05764939072676	34.13565108328574
5	11.708252744809139	39.548448300961944	34.78215102169451	13.961147932534407
6	31.503993831278425	38.47829669055127	13.187882045596707	16.8298274325736
7	24.559030794014934	35.057791770451885	20.734103463666774	19.649073971866407
8	27.589575244485186	34.37523296682553	18.561836794440474	19.47335499424882
9	24.28380598602759	14.552199766753215	22.323893077625744	38.84010116959345
10	16.491475613872584	28.72275787927799	32.30333600495289	22.48243050189653
11	33.09695099417843	23.003124355091835	23.349475034755052	20.550449615974685
12	21.69159911628584	28.608630131069333	30.337311966775932	19.362458785868892
13	32.62431429764401	21.617945604545913	25.948784893031235	19.80895520477884
14	20.081140445600546	23.69441791488892	29.089137706790552	27.135303932719978
15	21.994627964977788	30.913485919605048	25.914421786337627	21.17746432907954
16	20.865924692323812	28.869872930094754	27.000123182458818	23.264079195122616
17	21.19343005556009	27.493460931163405	27.39229133771351	23.920817675563
18	22.596526254661338	25.684022595182448	30.77101020814636	20.948440942009857
19	22.314038480920317	24.98610597850866	32.42360687838052	20.276248662190504
20	23.09226366165458	24.62049404164847	31.73829646658316	20.54894583011379
21	21.90155321882164	25.410717513826032	32.66814805571687	20.01958121163546
22	20.285751309013598	26.090684686500644	32.05690709642947	21.56665690805629
23	21.64574964525052	26.060864932833567	32.62920960055288	19.664175821363038
24	21.10672240272985	26.245926580055002	30.69767665084492	21.94967436637023
25	21.126303614365312	26.38302705694709	32.052651702397576	20.438017626290016
26	20.40547825989579	27.66415662409673	31.826411918942743	20.10395319706474
27	19.24340373930752	27.336235320090417	31.543572195319385	21.87678874528267
28	20.081204436488246	27.190496073359157	31.3252352864952	21.403064203657397
29	20.287095117655245	26.89562606284865	30.422515833745273	22.39476298575083
30	20.400582956986927	27.369574572580863	32.6300094866491	19.59983298378311
31	21.62306487556172	27.010457710821978	31.995731807790573	19.370745605825732
32	20.538163365536747	27.061778402755447	30.948776894170273	21.451281337537534
33	20.14938672733003	26.736320747925497	31.873413225956625	21.240879298787853
34	19.400981300262842	27.14435864332919	32.89765137444428	20.557008681963687
35	18.824871338321422	29.43305673272126	31.428740547346056	20.313331381611256
36	21.483436758605574	26.993212166587476	31.143117220107857	20.38023385469909
37	20.350254123812768	28.068898988783996	31.018239002766006	20.562607884637227
38	19.951974838782956	26.19409396101995	32.645687254135005	21.20824394606208
39	19.448974466036038	25.937778460347243	33.46393873512412	21.1493083384926
40	18.096847008985918	26.228201104162768	33.467074288621305	22.20787759823001
41	19.41486732289322	25.25873915554425	33.75010598490775	21.57628753665478
42	18.56484436616226	27.300688381974407	32.65279024266945	21.48167700919389
43	18.402723452180407	27.242584655944995	32.159484489408705	22.19520740246589
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1267.0
1	1158.5
2	1050.0
3	2194.5
4	3339.0
5	3339.0
6	5004.0
7	6669.0
8	7379.5
9	8090.0
10	12455.5
11	16821.0
12	16821.0
13	30635.0
14	44449.0
15	79570.0
16	114691.0
17	103908.0
18	93125.0
19	93125.0
20	93582.5
21	94040.0
22	73034.5
23	52029.0
24	56375.5
25	60722.0
26	60722.0
27	66171.0
28	71620.0
29	74979.5
30	78339.0
31	84932.0
32	91525.0
33	91525.0
34	101861.5
35	112198.0
36	123016.5
37	133835.0
38	140488.5
39	147142.0
40	147142.0
41	152064.0
42	156986.0
43	167159.5
44	177333.0
45	191904.5
46	206476.0
47	206476.0
48	277922.5
49	349369.0
50	319974.5
51	290580.0
52	256862.0
53	223144.0
54	223144.0
55	196297.5
56	169451.0
57	143386.0
58	117321.0
59	104178.5
60	91036.0
61	91036.0
62	79373.5
63	67711.0
64	58447.0
65	49183.0
66	41374.0
67	33565.0
68	33565.0
69	28637.0
70	23709.0
71	19850.0
72	15991.0
73	12898.0
74	9805.0
75	9805.0
76	7893.5
77	5982.0
78	4641.0
79	3300.0
80	2531.5
81	1763.0
82	1763.0
83	1318.0
84	873.0
85	678.5
86	484.0
87	363.0
88	242.0
89	242.0
90	175.5
91	109.0
92	78.5
93	48.0
94	32.5
95	17.0
96	17.0
97	14.0
98	11.0
99	8.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3125445.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.88399188538251
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.1589908332369	31.479585621728322
2	12.979238777859656	10.872446633006314
3	4.758685515966538	5.979382366074892
4	2.2146881271670886	3.710399181876773
5	1.176398684298689	2.4636136473570476
6	0.7508688031421888	1.8869629714676588
7	0.5118668073882666	1.5007317644932767
8	0.35024779555723917	1.1735820661594014
9	0.2637867674997317	0.9943598546487038
>10	1.5709552705561605	12.359754745726006
>50	0.1490756986744215	4.361711401918981
>100	0.10117335126761307	8.093707448971182
>500	0.0068968364191060345	1.9343178966162327
>1k	0.006053889745635291	5.2093961140496035
>5k	6.89683641907818E-4	2.1716703149792687
>10k+	3.8315757883767665E-4	5.808377970926433
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	63006	2.0159049351372365	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	45177	1.4454581667570539	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	43916	1.405111912063722	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17256	0.5521133790548226	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	11612	0.37153109397221834	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9949	0.3183226708516707	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	9441	0.3020689853764824	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	9197	0.29426209707737616	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	8667	0.27730451183751437	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6955	0.2225283119683757	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6913	0.22118450332672626	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5638	0.18039031241951145	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	5632	0.1801983397564187	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	5269	0.16858399363930576	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	4588	0.14679509637827573	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4404	0.1409079347100973	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4128	0.1320771922078296	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	3941	0.12609404420810477	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	3934	0.1258700761011632	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	3907	0.1250061991172457	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	3748	0.11991892354528715	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3723	0.11911903744906725	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3696	0.11825516046514978	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	3541	0.11329586666858639	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3413	0.1092004498559405	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3409	0.10907246808054534	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	3.1995443848795934E-5	0.0
9	6.399088769759187E-5	0.0	0.0	1.2798177539518374E-4	0.0
10	6.399088769759187E-5	0.0	0.0	1.5997721924397966E-4	0.0
11	6.399088769759187E-5	0.0	0.0	1.919726630927756E-4	0.0
12	9.59863315463878E-5	0.0	0.0	5.11927101580735E-4	0.0
13	9.59863315463878E-5	0.0	0.0	5.439225454295308E-4	0.0
14	9.59863315463878E-5	0.0	0.0	6.719043208247145E-4	0.0
15	9.59863315463878E-5	0.0	0.0	8.318815400686942E-4	0.0
16	9.59863315463878E-5	0.0	0.0	0.001407799529347021	0.0
17	9.59863315463878E-5	0.0	0.0	0.0019517220747765518	0.0
18	9.59863315463878E-5	0.0	0.0	0.002303671957113307	0.0
19	9.59863315463878E-5	0.0	0.0	0.003167548941030797	0.0
20	9.59863315463878E-5	0.0	0.0	0.0055032163419929	0.0
21	9.59863315463878E-5	0.0	0.0	0.01007856481237072	0.0
22	9.59863315463878E-5	0.0	0.0	0.015453799378968435	0.0
23	9.59863315463878E-5	0.0	0.0	0.018173412106116088	0.0
24	9.59863315463878E-5	0.0	0.0	0.022268828918761967	0.0
25	9.59863315463878E-5	0.0	0.0	0.025180414309002398	0.0
26	9.59863315463878E-5	0.0	0.0	0.032027439292644726	0.0
27	9.59863315463878E-5	0.0	0.0	0.08763552070185206	0.0
28	9.59863315463878E-5	0.0	0.0	0.22627177889868483	0.0
29	9.59863315463878E-5	0.0	0.0	0.4402893028032808	0.0
30	9.59863315463878E-5	0.0	0.0	0.7365031219554335	0.0
31	9.59863315463878E-5	0.0	0.0	1.1407335595411214	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13360	0.0	25.049776	1
GTATTGG	920	0.0	22.923912	1
ATTGGAC	990	0.0	21.863636	3
TTGGACC	1450	0.0	19.903448	4
GGACCCT	1395	0.0	19.096773	6
GACCCTC	1360	0.0	19.044117	7
GGGTACG	50	0.007037524	18.499998	1
TGGACCC	1635	0.0	17.764524	5
GAACCGT	75	2.0686995E-4	17.266666	6
TATTGGA	1355	0.0	15.97417	2
GTATCAA	21800	0.0	15.368578	2
ACCCTCG	1650	0.0	15.024243	8
CGACGAG	185	3.0559022E-10	15.000001	24
ATACACA	1210	0.0	14.830578	37
TGAACCG	75	0.004106506	14.8	5
CCCTCGC	1650	0.0	14.68788	9
ACACCGA	190	4.5656634E-10	14.605264	6
TACGTTA	115	2.2121389E-5	14.47826	19
TATACTG	375	0.0	14.306667	5
ACTCTGT	1850	0.0	14.1	37
>>END_MODULE