FastQCFastQC Report
Wed 25 May 2016
SRR2088560_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088560_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2867645
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT729822.545015160523705No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT510861.781461791818722No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT510091.7787766616858085No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211030.7359000155179598No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG132480.4619818701408298No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA114150.3980618242495148No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA106850.3726053957166944No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG106000.3696412910245166No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA95070.33152639186510185No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA87250.30425662869706677No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG80960.2823222539749516No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT64520.22499298204624352No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC61980.21613553978961833No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA54770.19099295763596957No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT53720.18733141654563237No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA49310.1719529439662162No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT48720.16989550659164576No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45430.15842267784192254No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG42980.14987908196446909No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA42070.14670574635284353No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG41980.14639189997367177No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT41460.14457856533845717No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA38670.13484932758413262No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA38360.13376830116698546No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT37640.13125753013361136No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37520.1308390682947157No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA37380.13035086281600408No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT35340.12323701155477754No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT34160.11912213680563667No Hit
GAGTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA33200.11577444209447126No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC32630.11378674835971676No Hit
GTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAA31640.1103344381888274No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC31050.108277000814257No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29910.10430161334474804No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAAAAAAA29800.10391802332576033No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29370.10241853506971749No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG29080.10140725229238626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG11450.023.2663751
ATTGGAC12100.022.4752063
GGTATCA180000.021.4394441
CCGTATA1303.274181E-1119.9230772
TTGGACC19100.019.7591634
TATTGGA13650.019.6520142
GGACCCT19300.019.266846
CAATGCG1355.638867E-1119.18518619
TGGACCC19650.018.7353695
GTATAAC1205.182301E-918.51
GACCCTC20500.017.4170747
CGTATAC1301.3955287E-817.0769233
ACGTTTA904.4496875E-516.44444526
CGTGCTA803.3847717E-416.187510
TATACTG2550.015.9607865
TCTATAC2550.015.9607863
CGAATTA1403.4788172E-815.85714215
ACTCTGT15500.015.63548537
TATACAC17800.015.38202337
TACGTTA1353.9778752E-715.07407519
Produced by FastQC (version 0.11.5)