##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088560_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2867645
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75302730986576	31.0	31.0	34.0	30.0	34.0
2	31.891990117326237	31.0	31.0	34.0	30.0	34.0
3	31.992676917819324	33.0	31.0	34.0	30.0	34.0
4	35.65636436867185	37.0	35.0	37.0	33.0	37.0
5	35.606670630430195	37.0	35.0	37.0	33.0	37.0
6	35.68375618320957	37.0	35.0	37.0	33.0	37.0
7	35.509329083620884	37.0	35.0	37.0	33.0	37.0
8	35.58477461470998	37.0	35.0	37.0	33.0	37.0
9	37.16152313134994	39.0	37.0	39.0	33.0	39.0
10	37.05404748495717	39.0	37.0	39.0	33.0	39.0
11	37.114586359190206	39.0	37.0	39.0	33.0	39.0
12	36.956977589624934	39.0	37.0	39.0	33.0	39.0
13	37.01352503535131	39.0	37.0	39.0	33.0	39.0
14	37.952298837547886	40.0	37.0	41.0	33.0	41.0
15	37.9645695335371	40.0	37.0	41.0	33.0	41.0
16	37.86287877334886	40.0	37.0	41.0	32.0	41.0
17	37.97505653593802	40.0	37.0	41.0	33.0	41.0
18	37.97940435444415	40.0	37.0	41.0	33.0	41.0
19	38.069563352507025	40.0	37.0	41.0	33.0	41.0
20	37.982102387150434	40.0	37.0	41.0	33.0	41.0
21	37.85755559003991	40.0	37.0	41.0	32.0	41.0
22	37.75136252918335	40.0	37.0	41.0	32.0	41.0
23	37.557917036453254	40.0	36.0	41.0	32.0	41.0
24	37.67794967647669	40.0	37.0	41.0	32.0	41.0
25	37.60255819670845	40.0	36.0	41.0	32.0	41.0
26	37.381195022396426	39.0	36.0	41.0	32.0	41.0
27	37.3312244019047	39.0	36.0	41.0	32.0	41.0
28	37.14928416871684	39.0	35.0	41.0	31.0	41.0
29	36.9009856519897	39.0	35.0	41.0	31.0	41.0
30	36.68225216161694	39.0	35.0	40.0	30.0	41.0
31	36.3268664705708	38.0	35.0	40.0	30.0	41.0
32	36.147732372730935	38.0	35.0	40.0	30.0	41.0
33	35.94931834310035	38.0	35.0	40.0	30.0	41.0
34	35.71845050555421	38.0	35.0	40.0	29.0	41.0
35	35.48384545506853	38.0	35.0	40.0	27.0	41.0
36	35.34522962221614	38.0	35.0	40.0	26.0	41.0
37	35.132744813252685	38.0	34.0	40.0	25.0	41.0
38	34.9425179895001	38.0	34.0	40.0	24.0	41.0
39	34.74446069858717	38.0	34.0	40.0	23.0	41.0
40	34.47761141982358	38.0	33.0	40.0	22.0	41.0
41	34.24055104449818	38.0	33.0	40.0	21.0	41.0
42	33.85144256001004	38.0	33.0	40.0	18.0	41.0
43	33.398943732574985	37.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	4.0
14	11.0
15	16.0
16	31.0
17	67.0
18	198.0
19	401.0
20	915.0
21	1893.0
22	3552.0
23	6383.0
24	10614.0
25	16513.0
26	24243.0
27	34468.0
28	47405.0
29	61949.0
30	76959.0
31	93669.0
32	114200.0
33	141086.0
34	175055.0
35	216743.0
36	286008.0
37	435479.0
38	510636.0
39	609145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.88157878677451	21.126429526667355	15.965435052107216	21.026556634450916
2	18.468359926001998	24.851786047436136	35.8223559750248	20.857498051537064
3	19.45282627382399	24.251014334061573	35.077842619989575	21.21831677212486
4	12.82170561558352	16.14188646084156	35.809558017118576	35.226849906456344
5	11.422613329055723	39.864174261458444	34.450463708025225	14.262748701460607
6	31.196643936052055	38.36810344376658	12.48393716795489	17.95131545222648
7	23.817418125325833	35.67526663865297	20.463725461136228	20.043589774884968
8	28.15334534086332	33.93878949451554	18.169368942111035	19.738496222510108
9	23.93082128366656	14.500574513232984	22.76174352125176	38.80686068184869
10	16.766196652654006	28.871146881849043	31.50797954419044	22.854676921306506
11	32.307171912841376	23.160990987378145	23.5356189486495	20.99621815113098
12	21.964015769036962	29.34697286449334	30.094485196040655	18.594526170429045
13	33.22600949559656	21.51737749965564	26.214681384899457	19.04193161984834
14	19.846424505125285	24.245888176535104	29.76853829536083	26.139149022978785
15	21.569824716797232	31.276639890920947	26.65493811123762	20.4985972810442
16	20.084599035096744	29.04585469958799	27.48987409529422	23.379672170021045
17	20.80627134809225	27.3708217021284	27.633859839694242	24.189047110085106
18	22.706436814877716	25.076534926743026	31.62190577982979	20.59512247854947
19	22.16139026971609	24.361592874989757	33.75804885193251	19.718968003361642
20	23.031477048239932	24.18950044374391	32.95836827780287	19.820654230213293
21	21.42827999979077	25.174978074343237	34.16796709495073	19.228774830915263
22	19.502169898993774	25.994605329460235	33.3162926373383	21.186932134207687
23	21.101949509091956	25.983097628890604	34.210824561617635	18.704128300399805
24	20.548115265313523	26.166662888886176	31.755116131878246	21.53010571392205
25	20.458390072690307	26.366304057859324	33.23413462963512	19.94117123981525
26	19.744529047354188	27.967757515313085	33.0910206807328	19.19669275659993
27	18.39247884588225	27.528825918131428	32.77748814794021	21.301207088046116
28	19.34960568689639	27.15726667701197	32.55908593985657	20.934041696235063
29	19.57951559554966	26.989812197813883	31.399737415196093	22.030934791440362
30	19.62802229704165	27.38944325395926	34.17166350786098	18.81087094113811
31	20.854010869546265	27.159149755287004	33.57929590308424	18.407543472082494
32	19.832719879901454	27.166368222007954	32.21783031023715	20.783081587853445
33	19.401006749440743	26.838573114873004	33.205923327329565	20.554496808356685
34	18.26223259852597	27.655131649838104	34.12005321439718	19.96258253723874
35	17.55224234519963	30.414852605535202	32.41747147921029	19.615433570054872
36	20.870017034884025	27.446493551328704	32.004484516040165	19.679004897747106
37	19.59137201431837	28.68301341344553	31.880829042646493	19.84478552958961
38	19.14602400227364	26.57281497535434	33.72139856920923	20.559762453162786
39	18.582844110759876	26.314310174376537	34.64459512945292	20.458250585410674
40	17.04555480193678	26.644511437085132	34.464238076888876	21.845695684089208
41	18.632083120469932	25.730346678197613	34.6922300354472	20.94534016588525
42	17.670457814687662	28.036420128711885	33.48964045410084	20.803481602499613
43	17.55475311623301	28.11108069513486	32.69731783397178	21.63684835466036
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	757.0
1	813.0
2	869.0
3	2288.5
4	3708.0
5	3708.0
6	5757.5
7	7807.0
8	8750.5
9	9694.0
10	15134.5
11	20575.0
12	20575.0
13	37651.5
14	54728.0
15	94558.0
16	134388.0
17	120058.5
18	105729.0
19	105729.0
20	103584.5
21	101440.0
22	75510.5
23	49581.0
24	53271.5
25	56962.0
26	56962.0
27	60539.5
28	64117.0
29	65616.0
30	67115.0
31	70238.0
32	73361.0
33	73361.0
34	81164.5
35	88968.0
36	97953.0
37	106938.0
38	113185.5
39	119433.0
40	119433.0
41	124665.5
42	129898.0
43	139962.5
44	150027.0
45	167346.0
46	184665.0
47	184665.0
48	265518.0
49	346371.0
50	314910.0
51	283449.0
52	248458.5
53	213468.0
54	213468.0
55	182922.5
56	152377.0
57	125612.0
58	98847.0
59	85991.0
60	73135.0
61	73135.0
62	63379.0
63	53623.0
64	46141.0
65	38659.0
66	32816.5
67	26974.0
68	26974.0
69	22833.5
70	18693.0
71	15599.0
72	12505.0
73	10268.5
74	8032.0
75	8032.0
76	6402.5
77	4773.0
78	3768.5
79	2764.0
80	2163.5
81	1563.0
82	1563.0
83	1182.5
84	802.0
85	635.0
86	468.0
87	329.5
88	191.0
89	191.0
90	160.0
91	129.0
92	82.5
93	36.0
94	27.5
95	19.0
96	19.0
97	11.5
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2867645.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.58004565298246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.16244060348058	30.54092921781309
2	11.983632873634539	9.48625472454073
3	4.23582393880358	5.02962314627525
4	1.9019232465835627	3.0111283571298415
5	1.083520533982914	2.1442896100493836
6	0.659824613716813	1.5669533000323783
7	0.481388766991561	1.3337372562051246
8	0.3346925462418081	1.0597717007970968
9	0.2582726427981714	0.920019869358125
>10	1.5868623059951188	12.118531237445255
>50	0.18044604204809103	4.96385097423152
>100	0.11369143075362685	8.597922605187923
>500	0.008563657232580561	2.334701193118634
>1k	0.007592520845380568	6.690417636130949
>5k	6.179958827635347E-4	1.741119471211432
>10k+	7.06281008872611E-4	8.460749700473269
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	72982	2.545015160523705	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	51086	1.781461791818722	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	51009	1.7787766616858085	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21103	0.7359000155179598	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	13248	0.4619818701408298	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	11415	0.3980618242495148	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	10685	0.3726053957166944	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	10600	0.3696412910245166	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	9507	0.33152639186510185	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8725	0.30425662869706677	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8096	0.2823222539749516	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6452	0.22499298204624352	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	6198	0.21613553978961833	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5477	0.19099295763596957	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	5372	0.18733141654563237	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4931	0.1719529439662162	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	4872	0.16989550659164576	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4543	0.15842267784192254	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	4298	0.14987908196446909	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4207	0.14670574635284353	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	4198	0.14639189997367177	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	4146	0.14457856533845717	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	3867	0.13484932758413262	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3836	0.13376830116698546	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	3764	0.13125753013361136	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3752	0.1308390682947157	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3738	0.13035086281600408	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT	3534	0.12323701155477754	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3416	0.11912213680563667	No Hit
GAGTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA	3320	0.11577444209447126	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3263	0.11378674835971676	No Hit
GTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAA	3164	0.1103344381888274	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3105	0.108277000814257	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2991	0.10430161334474804	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAAAAAAA	2980	0.10391802332576033	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2937	0.10241853506971749	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2908	0.10140725229238626	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.487181990797327E-5	0.0
2	0.0	0.0	0.0	3.487181990797327E-5	0.0
3	0.0	0.0	0.0	3.487181990797327E-5	0.0
4	0.0	0.0	0.0	3.487181990797327E-5	0.0
5	0.0	0.0	0.0	3.487181990797327E-5	0.0
6	0.0	0.0	0.0	3.487181990797327E-5	0.0
7	0.0	0.0	0.0	3.487181990797327E-5	0.0
8	0.0	0.0	0.0	3.487181990797327E-5	0.0
9	0.0	0.0	0.0	6.974363981594653E-5	0.0
10	6.974363981594653E-5	0.0	0.0	1.7435909953986633E-4	0.0
11	6.974363981594653E-5	0.0	0.0	5.579491185275723E-4	0.0
12	6.974363981594653E-5	0.0	3.487181990797327E-5	9.764109574232515E-4	0.0
13	6.974363981594653E-5	0.0	3.487181990797327E-5	0.0010112827773312248	0.0
14	1.3948727963189307E-4	0.0	3.487181990797327E-5	0.0013251291565029842	0.0
15	1.3948727963189307E-4	0.0	3.487181990797327E-5	0.001848206455122583	0.0
16	1.3948727963189307E-4	0.0	3.487181990797327E-5	0.0028594892324538077	0.0
17	1.3948727963189307E-4	0.0	3.487181990797327E-5	0.004254362028772739	0.0
18	1.3948727963189307E-4	0.0	3.487181990797327E-5	0.004916926607024231	0.0
19	2.0923091944783962E-4	0.0	3.487181990797327E-5	0.006137440303803295	0.0
20	2.0923091944783962E-4	0.0	3.487181990797327E-5	0.010984623271011578	0.0
21	2.0923091944783962E-4	0.0	3.487181990797327E-5	0.019144629129477325	0.0
22	2.0923091944783962E-4	0.0	3.487181990797327E-5	0.03019899604030485	0.0
23	2.0923091944783962E-4	0.0	3.487181990797327E-5	0.036894385462635716	0.0
24	2.0923091944783962E-4	0.0	3.487181990797327E-5	0.04554259679981309	0.0
25	2.0923091944783962E-4	0.0	3.487181990797327E-5	0.05007593338784961	0.0
26	2.0923091944783962E-4	0.0	3.487181990797327E-5	0.05980517114217415	0.0
27	2.0923091944783962E-4	0.0	3.487181990797327E-5	0.11755290490977788	0.0
28	2.0923091944783962E-4	0.0	3.487181990797327E-5	0.2786955847045223	0.0
29	2.0923091944783962E-4	0.0	3.487181990797327E-5	0.5524742428020205	0.0
30	2.0923091944783962E-4	0.0	3.487181990797327E-5	0.9219760465469052	0.0
31	2.0923091944783962E-4	0.0	3.487181990797327E-5	1.3856317640433178	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTGG	1145	0.0	23.266375	1
ATTGGAC	1210	0.0	22.475206	3
GGTATCA	18000	0.0	21.439444	1
CCGTATA	130	3.274181E-11	19.923077	2
TTGGACC	1910	0.0	19.759163	4
TATTGGA	1365	0.0	19.652014	2
GGACCCT	1930	0.0	19.26684	6
CAATGCG	135	5.638867E-11	19.185186	19
TGGACCC	1965	0.0	18.735369	5
GTATAAC	120	5.182301E-9	18.5	1
GACCCTC	2050	0.0	17.417074	7
CGTATAC	130	1.3955287E-8	17.076923	3
ACGTTTA	90	4.4496875E-5	16.444445	26
CGTGCTA	80	3.3847717E-4	16.1875	10
TATACTG	255	0.0	15.960786	5
TCTATAC	255	0.0	15.960786	3
CGAATTA	140	3.4788172E-8	15.857142	15
ACTCTGT	1550	0.0	15.635485	37
TATACAC	1780	0.0	15.382023	37
TACGTTA	135	3.9778752E-7	15.074075	19
>>END_MODULE